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Preparation method of molecularly imprinted electrochemical sensor for identifying glycine

A technology of molecular imprinting and electrochemistry, applied in the direction of electrochemical variables of materials, nanotechnology for sensing, scientific instruments, etc., can solve problems affecting the absorption of other amino acids, breaking absorption balance, interfering with chromatographic analysis, etc., to achieve excellent electrochemistry Chemical properties, good reproducibility, and the effect of enhancing electrical signals

Pending Publication Date: 2021-10-22
ZHEJIANG SCI-TECH UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

b class="d_n">[0002] Glycine is a non-essential amino acid for the human body. If the human body consumes too much glycine, it will not only be unable to be absorbed and utilized by the human body, but will also break the body's absorption balance of amino acids and affect other amino acids Absorption, leading to nutritional imbalance and affecting health
Milk-containing beverages produced with glycine as the main raw material are likely to have adverse effects on the normal growth and development of adolescents and children
At present, formaldehyde method, Kjeldahl method and liquid chromatography are commonly used. The titration end point of formaldehyde method is difficult to grasp, and its accuracy is not good; The sample cannot contain other nitrogen compounds; the by-products formed by the derivatization of liquid chromatography may interfere with the chromatographic analysis. In addition, the derivatization agent is expensive and the operation is complicated, so it is not suitable for the analysis of an amino acid

Method used

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  • Preparation method of molecularly imprinted electrochemical sensor for identifying glycine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Step 1: Preparation of MWNTs-CS nanocomposite suspension: 10 mg of multi-walled carbon nanotube fine powder was weighed and dispersed in 10 mL of 1% acetic acid solution containing 1.0 mg chitosan. The ultrasonic treatment time was 120 min.

[0028] Step 2: Preparation of MWNTs-CS / GCE composite modified electrode: Polish the glassy carbon electrode with 20 nm alumina suspension slurry until the surface is smooth, ultrasonically rinse with absolute ethanol and deionized water for 30 s, and then wash with 0.5 M Soak in sulfuric acid solution, followed by ultrasonic cleaning with deionized water for 30 s. Cyclic voltammetry was used on the electrochemical workstation to scan from -0.4 V to 1.6 V at a scanning speed of 0.2 V / s. After 40 cycles of cyclic activation, 5 μL of MWNTs-CS nanocomposite suspension was dropped into The surface of the pretreated glassy carbon electrode was dried overnight at 37 °C to obtain a MWNTs-CS / GCE composite modified electrode.

[0029] Step...

Embodiment 2

[0032] Step 1: Preparation of MWNTs-CS nanocomposite suspension: 10 mg of multi-walled carbon nanotube fine powder was weighed and dispersed in 10 mL of 1% acetic acid solution containing 1.0 mg chitosan. The ultrasonic treatment time was 120 min.

[0033] Step 2: Preparation of MWNTs-CS / GCE composite modified electrode: Polish the glassy carbon electrode with 20 nm alumina suspension slurry until the surface is smooth, ultrasonically rinse with absolute ethanol and deionized water for 30 s, and then wash with 0.5 M Soak in sulfuric acid solution, followed by ultrasonic cleaning with deionized water for 30 s. Cyclic voltammetry was used on the electrochemical workstation to scan from -0.4 V to 1.6 V at a scanning speed of 0.2 V / s. After 40 cycles of activation, the glassy carbon electrode was thoroughly cleaned, and then 5 μL of The MWNTs-CS nanocomposite suspension was dropped onto the surface of the pretreated glassy carbon electrode and dried overnight at 37 °C to obtain t...

Embodiment 3

[0037] Step 1: Preparation of MWNTs-CS nanocomposite suspension: 10 mg of multi-walled carbon nanotube fine powder was weighed and dispersed in 10 mL of 1% acetic acid solution containing 1.0 mg chitosan. Sonication time is 120 min

[0038]Step 2: Preparation of MWNTs-CS / GCE composite modified electrode: Polish the glassy carbon electrode with 20 nm alumina suspension slurry until the surface is smooth, ultrasonically rinse with absolute ethanol and deionized water for 30 s, and then wash with 0.5 M Soak in sulfuric acid solution, followed by ultrasonic cleaning with deionized water for 30 s. Cyclic voltammetry was used on the electrochemical workstation to scan from -0.4 V to 1.6 V at a scanning speed of 0.2 V / s. After 40 cycles of cyclic activation, 5 μL of MWNTs-CS nanocomposite suspension droplets MWNTs-CS / GCE composite modified electrode was obtained by inserting it into the surface of the pretreated glassy carbon electrode and drying it overnight at 37 °C.

[0039] Ste...

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Abstract

The invention relates to the field of electrochemical sensors, and discloses a preparation method of a molecularly imprinted electrochemical sensor for identifying glycine, which comprises the following steps: preparing a uniform MWNTs-CS nano composite material, dripping the nano composite material on a glassy carbon electrode to obtain an MWNTs-CS / GCE composite modified electrode, and synthesizing an MIP molecular identification element on the surface of the MWNTs-CS / GCE by using cyclic voltammetry; finally, cleaning with a phosphate buffer solution to obtain the molecularly imprinted electrochemical sensor capable of detecting the glycine content. The MWNTs-CS / GCE composite modified electrode is used for the first time, the MWNTs-CS nanocomposite can effectively modify the working electrode, electric signals can be greatly enhanced, and a novel sensor with excellent electrochemical performance is prepared.

Description

technical field [0001] The invention relates to the field of electrochemical sensors, in particular to a preparation method of a molecularly imprinted electrochemical sensor for recognizing glycine. Background technique [0002] Glycine is a non-essential amino acid for the human body. If the human body consumes too much glycine, it will not only be unable to be absorbed and utilized by the human body, but will also break the body's absorption balance of amino acids and affect the absorption of other amino acids, resulting in nutritional imbalance and affecting health. Milk-containing beverages produced with glycine as the main raw material are likely to have adverse effects on the normal growth and development of adolescents and children. At present, formaldehyde method, Kjeldahl method and liquid chromatography are commonly used. The titration end point of formaldehyde method is difficult to grasp, and its accuracy is not good; The sample cannot contain other nitrogen c...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30B82Y15/00B82Y40/00
CPCG01N27/26G01N27/308B82Y15/00B82Y40/00
Inventor 陈栩诗王俊森陈浩东周骏懿王秉
Owner ZHEJIANG SCI-TECH UNIV
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