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Method for ratio-type electrochemical detection of nitrite ions

A nitrite ion and electrochemical technology, applied in the field of analytical chemistry, can solve the problems of strict detection environment conditions, complicated detection operation steps, expensive detection equipment, etc., and achieve low detection cost, mild conditions, and low-cost analysis Effect

Active Publication Date: 2021-11-02
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] (1) Some testing instruments and equipment are relatively expensive, and the testing operation steps are relatively complicated;
[0010] (2) The cost of some detection reagents is relatively high, and the detection environment conditions are strict

Method used

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  • Method for ratio-type electrochemical detection of nitrite ions
  • Method for ratio-type electrochemical detection of nitrite ions
  • Method for ratio-type electrochemical detection of nitrite ions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] OPD probe and NO 2 - Applied experiments to investigate the electrochemical behavior of

[0037] (1) Add 1000 μL of acetate buffer solution (0.1M) with pH=3 into a 1mL centrifuge tube, shake well, and measure the electrochemical signal by using the cyclic voltammetry (CV) of the electrochemical detection device (potential window: 0-1.0V; scan rate: 50mV / s);

[0038](2) Add 50 μL of 10 mM OPD solution to 950 μL of acetate buffer (0.1 M) at pH=3, shake well, and measure the electrochemical signal (potential window: 0-1.0V; scan rate: 50mV / s);

[0039] (3) Take 50μL of 5mM NO 2 - The solution was added to 950 μL of acetate buffer (0.1M) with pH=3, and after shaking well, the electrochemical signal was measured by cyclic voltammetry (CV) of the electrochemical detection device (potential window: 0-1.0V; scanning Rate: 50mV / s).

[0040] figure 1 Recorded for acetate buffer, acetate buffer + OPD, acetate buffer + NO 2 - The cyclic voltammogram. It can be seen from ...

Embodiment 2

[0042] NO 2 - For OPD probe +NO 2 - system impact

[0043] (1) Add 50 μL of 10 mM OPD solution and 900 μL of acetate buffer (0.1M) with pH=3 to a 1 mL centrifuge tube, and shake well;

[0044] (2) Add 50 μL of different concentrations of NO to the above mixed solution 2 - The solution was shaken evenly, and after reacting for 1 min, the electrochemical signal was measured by linear sweep voltammetry (LSV) of the electrochemical detection device (potential window: 0-1.0V; scan rate: 50mV / s).

[0045] The result is as figure 2 as shown, figure 2 with NO 2 - With the increase of the concentration, the oxidation signal of OPD decreased gradually, and the NO 2 - The oxidation signal gradually increased. This is because the diazotization reaction between nitrite ions and OPD will occur in acidic medium, resulting in a decrease in the concentration of free OPD.

Embodiment 3

[0047] Different electrode pair OPD probe+NO 2 - Ratiometric electrochemical response of the system

[0048] (1) Add 50 μL of 10 mM OPD solution and 900 μL of acetate buffer (0.1M) with pH=3 to a 1 mL centrifuge tube, and shake well;

[0049] (2) Add 50 μL of 5 mM NO to the above mixed solution 2 - The solution was shaken evenly, and after reacting for 1 min, using different electrodes, the electrochemical signal was measured by differential pulse voltammetry (DPV) of the electrochemical detection device (initial potential: 0V; final potential: 1.0V; potential increase range: 4mV; amplitude : 50mV; pulse duration: 50ms; pulse period: 0.5s), record NO 2 - and the oxidation signal value of the OPD probe.

[0050] The result is as image 3 As shown, compared with the bare carbon electrode, the electrodes modified by Au, Pd and Pt are more effective for OPD probe + NO 2 - The ratiometric electrochemical response of the system has been improved to varying degrees, because ...

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Abstract

The invention belongs to the technical field of analytical chemistry, and relates to a method for ratio-type electrochemical detection of nitrite ions, wherein the method comprises the steps: adding 50 [mu]L of a 10 mM o-phenylenediamine solution and 900 [mu]L of a 0.1 M acetate buffer solution into a 1 mL centrifuge tube, and uniformly shaking; respectively adding 50 [mu]L of NO2<-> with different concentrations, wherein the final concentrations in the system are respectively 10 [mu]M, 20 [mu]M, 50 [mu]M, 100 [mu]M, 200 [mu]M and 300 [mu]M; measuring the oxidation signal value of the mixed solution by using differential pulse voltammetry, recording the oxidation signal values of NO2<-> and OPD probes, and drawing a standard working curve by taking the concentration of NO2<-> as a horizontal coordinate and the ratio (INitrite / IOPD) of the NO2<-> and the OPD as a vertical coordinate; and determining oxidation signal values of a sample to be detected and the OPD probes in the mixed solution by using differential pulse voltammetry, and comparing the oxidation signal values with a standard working curve to obtain the concentration of NO2<->. The detection condition is mild, 10-300 [mu]M can be detected, the detection limit is as low as 4.7 [mu]M, and high-sensitivity, high-accuracy, high-selectivity and low-cost detection of nitrite ions in the environment and food is achieved.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry and relates to the detection of nitrite ions, in particular to a ratio-type electrochemical detection method for nitrite ions. Background technique [0002] Nitrite is often used as a preservative or coloring agent in food processing. However, excessive nitrite in food and drinking water will pose a huge threat to human health. It can react with amino acids to form nitrosamines with strong carcinogenic effects. In addition, long-term intake of foods containing excess nitrite will reduce the oxygen-carrying capacity of hemoglobin, leading to tissue hypoxia. In view of its hazards, the World Health Organization has stipulated that the concentration of nitrite ions in drinking water should not exceed 65 μM. Therefore, the development of reliable, convenient and low-cost nitrite detection methods is of great significance to ensure food safety and human health. [0003] At present, the d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 牛湘衡汪梦珠朱恒佳刘朋刘邦祥赵梦豪
Owner JIANGSU UNIV
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