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Cytokinin oxidase activity detection kit and cytokinin oxidase activity detection method

A cell division and oxidase technology, applied in the field of cytokinin oxidase activity detection kits, can solve the problems of stagnant research progress and inability to study the production process of plant hormones in depth, and achieve wide applicability and unlimited Impurity interference, no false positive effect

Pending Publication Date: 2021-11-02
苏州梦犀生物医药科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, there is no suitable detection method in the prior art that can detect the activity of cytokinin oxidase, so researchers in the field of plant agronomy cannot further study the production process of plant hormones, and research progress has stagnated

Method used

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  • Cytokinin oxidase activity detection kit and cytokinin oxidase activity detection method
  • Cytokinin oxidase activity detection kit and cytokinin oxidase activity detection method
  • Cytokinin oxidase activity detection kit and cytokinin oxidase activity detection method

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Embodiment 1

[0030] A cytokinetin oxidase activity detection kit, including the following reagents:

[0031] Reagent 1: 20mmol / L pH 6.0 phosphate buffered solution, 4 ° C preservation;

[0032] 2: 1 mmol / L chloride solution, 4 ° C preservation;

[0033] Exconduration 3: 1 mmol / L transcorin (Tz) solution, 4 ° C preservation;

[0034] Reagent 4: Save acetic acid, 4 ° C.

[0035] A method of detection of cytokinetin oxidase activity, using the above cytokine oxidase activity detection kit, including the following steps:

[0036] (1) Preparing for test supplies: high performance liquid chromatograph, low speed centrifuge, nitrogen blower, solvent extraction unit, needle filter (organic system, 50, 0.22 μm), filter (water system and organic system 1 A, 0.45 μm), C18 column (4.6 × 250 mm), an adjustable pipette, a sample bottle (50, 2 mL), methanol (color grade, 500 mL), and ultrapure water.

[0037] (2) Preparing before testing, including the following steps:

[0038] (1) Ultra-pure water 500...

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Abstract

The invention discloses a cytokinin oxidase activity detection kit and a cytokinin oxidase activity detection method, and belongs to the technical field of kits, the kit comprises a reagent I: a 20 mmol / L phosphoric acid buffer solution with pH of 6.0; a reagent II: 1mmol / L manganese chloride solution; a reagent III: a 1 mmol / L trans-zeatin (tZ) solution; and a reagent IV: acetic acid. Based on the principle that trans-zeatin (tZ) is degraded into adenine under the action of cytokinin oxidase, the activity of cytokinin oxidase can be calculated by detecting the tZ content and utilizing the variation of the tZ content before and after the reaction. The tZ content which can be detected in the method can reach ng / mL, the detection time is short, the cytokinin oxidase activity of each part of a plant body can be detected, and the applicability is wide.

Description

Technical field [0001] The present invention belongs to the technical field of kits, and more particularly to a cytokine oxidase activity detection kit and a detection method. Background technique [0002] With the rapid development of agriculture, research on the field of agricultural disciplines also develops rapidly. In the field of plant disciplines, plant hormones have always been the focus of research. Cytokinin, CTK is one of the five major plant hormones. It is a plant hormone that directly regulates crop yield, which is a trace organic substance synthesized by plants. In the plants, the plant growth has a significant impact on plant growth, participating in the development of plants, forming, resistance, anti-counterfeit biological processes, the main role is to promote cell division and expand, promote bud differentiation and lateral bud development , Delay aging and breaking sleep. [0003] Cytokinin oxidase / dehydrogenase, ckx) is a key enzyme of synthetic cell fract...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 王黔
Owner 苏州梦犀生物医药科技有限公司
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