Application of ROS response type nanoparticles coated with hematopoietic growth factors in preparation of drugs for treating hematopoietic injury

A technology of hematopoietic growth factor and hematopoietic injury, applied in the field of nanomedicine and biomedicine, can solve the problems of single effect and limited effect, and achieve the effect of low cost, improved bioavailability, and good disease treatment effect

Active Publication Date: 2021-11-05
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some studies use antioxidants to scavenge ROS to improve the hematopoietic system damage after radiation, but the effect is single and the effect is limited
There is no report on the treatment of hematopoietic injury by ROS-responsive nanoparticles coated with hematopoietic growth factors

Method used

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  • Application of ROS response type nanoparticles coated with hematopoietic growth factors in preparation of drugs for treating hematopoietic injury
  • Application of ROS response type nanoparticles coated with hematopoietic growth factors in preparation of drugs for treating hematopoietic injury
  • Application of ROS response type nanoparticles coated with hematopoietic growth factors in preparation of drugs for treating hematopoietic injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Coated with nanoparticles prepared ROS responsive hematopoietic growth factor, comprising the steps of:

[0029] (1) Take 70mg hydrogen peroxide-sensitive amphiphilic biomaterial (Chinese Patent No. Example 4 Preparation of the three-arm embodiment of the present invention in 201410606622.9 hydrogen peroxide-sensitive amphiphilic biomaterial PLGA-PEG) was dissolved in 2ml dichloro methane, to obtain a solution;

[0030] (2) 30mg of ovalbumin (OVA) was dissolved in 1mL distilled water to obtain a solution II; 100μL the erythropoietin (EPO) (10000IU / mL) was added to the solution II to obtain a solution three, 4 ℃ refrigerator;

[0031] (3) The solution was placed in a ice bath, using ultrasonic cell disrupter, a shear rate of 20%, and the solution was added dropwise to a solution of a three, sonicated 5min, an emulsion is formed;

[0032] (4) take 20mL concentration of 0.5% by mass aqueous solution of emulsifier (polyvinyl alcohol aqueous solution of an average molecular we...

Embodiment 2

[0035] Coated with nanoparticles prepared ROS responsive hematopoietic growth factor, comprising the steps of:

[0036] (1) Take 10mg hydrogen peroxide-sensitive amphiphilic biomaterial (prepared in Example 4 of the present invention in Chinese Patent No. 201410606622.9) was dissolved in 2ml of dichloromethane, to give a solution;

[0037] (2) 5mg of bovine serum albumin was dissolved in 1mL distilled water to obtain a solution II; the 1μL thrombopoietin (15000U / mL) was added to the solution II to obtain a solution three, 4 ℃ refrigerator;

[0038] (3) The solution was placed in a ice bath, using ultrasonic cell disrupter, a shear rate of 10%, and the solution was added dropwise to a solution of a three, sonicated 2min, an emulsion is formed;

[0039] Aqueous emulsifier solution (average molecular weight ~ 70,000 polystyrene sulfonate) (4) taking 1mL concentration of 5%, was placed in an ice bath conditions, using ultrasonic cell disrupter, a shear rate of 10%, the emulsion was ...

Embodiment 3

[0042] Coated with nanoparticles prepared ROS responsive hematopoietic growth factor, comprising the steps of:

[0043] (1) 100mg of hydrogen peroxide-sensitive amphiphilic biomaterial (prepared in Example 4 of the present invention in Chinese Patent No. 201410606622.9) was dissolved in 2ml of dichloromethane, to give a solution;

[0044] (2) 50mg hemocyanin was dissolved in 1mL distilled water to obtain a solution II; 2000μL the granulocyte - macrophage colony stimulating factor (150μg / mL) was added to the solution II to obtain a solution three refrigerated. 4 deg.] C;

[0045] (3) The solution was placed in a ice bath, using ultrasonic cell disrupter, a shear rate of 35%, and the solution was added dropwise to a solution of a three, the ultrasonic 10min, an emulsion is formed;

[0046] (4) take 1000mL emulsifier concentration of 0.1% by mass aqueous solution (average molecular weight of ethylene oxide - propylene oxide block copolymers and 12600), was placed under ice-cooling,...

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Abstract

The invention discloses application of ROS (reactive oxygen species) response type nanoparticles coated with hematopoietic growth factors in preparation of drugs for treating hematopoietic injury. Experiments prove that the bioavailability of the ROS response type nanoparticles coated with hematopoietic growth factors is improved, and the nanoparticles can be rapidly taken by cells to play a role. The nanoparticles deliver cytokines, can remove ROS (reactive oxygen species) generated by radiation, and can continuously release the cytokines to promote hematopoiesis. The hematopoietic microenvironment is improved, and the nanoparticles and the hematopoietic factor act at the same time to treat the hematopoietic injury after radiation. Meanwhile, animal experiment level proves that the nanoparticles have better disease treatment effect compared with free drugs, and the synergistic effect of the nanoparticles is shown.

Description

Technical field [0001] The present invention is in the biomedical field of nanotechnology medicine, relates to a hematopoietic growth factor coated with ROS-responsive nanoparticles in the manufacture of a medicament treatment of hematopoietic damage. Background technique [0002] As technology economic development, nuclear technology is widely used, while increasing the risk of public exposure to ionizing radiation. After the body exposed to ionizing radiation, the organizations of the system will be damaged, the hematopoietic system is particularly sensitive to ionizing radiation. Small doses of radiation greater than 1Gy's can cause hematopoietic tissue destruction, atrophy and aplastic, clinical manifestations of whole blood cells rapidly decreased hematopoietic stem cell update occurs and aplastic, hematopoietic progenitor cells not proliferate, resulting in white blood cells, red blood cells and platelets were reduced. After exposure to radiation, usually clinically used fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K9/51A61K47/34A61K47/42A61K38/19A61P7/00A61P7/06
CPCA61K9/19A61K9/5153A61K9/5169A61K38/19A61K38/193A61K38/196A61P7/06A61P7/00
Inventor 杨菁李慧洋梁晓玉
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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