Magnetoelectric response biomimetic hydrogel and adjustable cell electrical microenvironment magnetoelectric response biomimetic hydrogel and preparation method thereof

A hydrogel and microenvironment technology, applied in the field of biomedical materials and biomedical engineering, can solve the problems of limited application, limited wide application, low biological activity, etc., to increase the interface coupling area, enhance the stress conduction efficiency, and improve the magnetic field. The effect of the coefficient of electrical effect

Active Publication Date: 2022-04-15
SICHUAN UNIV
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  • Description
  • Claims
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Problems solved by technology

However, invasive electrical stimulation often needs to face two problems: (I) the contact problem of the electrode / tissue interface; (II) exogenous electrical stimulation often causes inflammation and gliosis due to the introduction of implanted electrodes, This is detrimental to the regeneration of nerve tissue
However, the simple mixture of magnetostrictive phase and piezoelectric phase to obtain magnetoelectric materials has problems such as small coupling area, weak conjugation, and low magnetoelectric conversion efficiency.
In addition, the matrix material used has poor biocompatibility, no biological activity, and its structure and mechanical properties do not match those of nerve tissue, which limits its application in the repair of various tissue injuries
[0006] In summary, the currently reported electrical stimulation methods have problems such as poor histocompatibility, mismatched mechanical properties, and low biological activity, which limit their wide application in the field of tissue engineering and regeneration.

Method used

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  • Magnetoelectric response biomimetic hydrogel and adjustable cell electrical microenvironment magnetoelectric response biomimetic hydrogel and preparation method thereof
  • Magnetoelectric response biomimetic hydrogel and adjustable cell electrical microenvironment magnetoelectric response biomimetic hydrogel and preparation method thereof
  • Magnetoelectric response biomimetic hydrogel and adjustable cell electrical microenvironment magnetoelectric response biomimetic hydrogel and preparation method thereof

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Experimental program
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Effect test

Embodiment 1-5

[0061] The magnetoelectric response biomimetic hydrogel provided by embodiment 1-5, its specific steps are as follows:

[0062] (1) Preparation of magnetic nanoparticles

[0063] Dissolve 1.350g of ferric chloride hexahydrate, 0.4g of sodium citrate and 3.854g of ammonium acetate in 70mL of ethylene glycol, and react at 170°C for 1h under continuous stirring until the color of the reaction solution becomes uniform black. Then transfer the reaction solution to a 100mL polytetrafluoroethylene autoclave, heat the autoclave to 200°C and keep it for 12h. Washed 3 times with ethanol to obtain magnetic Fe 3 o 4 nanoparticles, then the Fe 3 o 4 Nanoparticles dispersed in 20mL ethanol to form 10mg / mL Fe 3 o 4 Suspension, set aside.

[0064] (2) Preparation of magnetoelectric nanoparticles

[0065] (21) Preparation of Fe 3 o 4 @TiO 2 nanoparticles

[0066] Fe prepared by step (1) 3 o 4 Mix 5-10mL of suspension with 85mL of ethanol and 30mL of acetonitrile, and disperse by ...

Embodiment 6-8

[0091] The specific steps of the biomimetic hydrogel with adjustable cell electrical microenvironment and magnetoelectric response provided in this embodiment are as follows:

[0092] According to steps (1)-(3) in Example 1, magnetoelectric nanoparticles and formylated hyaluronic acid were prepared.

[0093] (4) Construction of magnetoelectric composite hydrogel loaded with cells

[0094] AHA was dissolved in phosphate-buffered saline (PBS) solution at a concentration of 4% w / v to form an aldylated hyaluronic acid solution. Then, the lyophilized collagen (Col) was dissolved in 0.4M acetic acid to form a collagen solution, and the pH of the collagen solution was adjusted to 7.5 at -4°C, and the final concentration of the collagen solution was 6 mg / ml. Mix the aldylated hyaluronic acid solution with the collagen solution at a volume ratio of 1:1, and add Fe 3 o 4 @BaTiO 3 Nanoparticle formation reaction mixture, Fe 3 o 4 @BaTiO 3 The concentration of nanoparticles in the ...

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Abstract

The invention discloses a magnetoelectric response bionic hydrogel and an adjustable cell electrical microenvironment magnetoelectric response bionic hydrogel and a preparation method thereof. The magnetoelectric response bionic hydrogel passes Fe 3 o 4 @BaTiO 3 Nanoparticles were incubated with a mixed solution of aldylated hyaluronic acid solution and collagen solution; tunable cell electrical microenvironment magnetoelectric response biomimetic hydrogel through Fe 3 o 4 @BaTiO 3 Nanoparticles are incubated with a mixed solution of aldylated hyaluronic acid solution and collagen solution and cells. The hydrogel has the ability to respond to radio stimulation by an external magnetic field, meets the good biocompatibility required for tissue regeneration, can effectively overcome the problems caused by traditional electrical stimulation, realize radio stimulation, and simulate the extracellular microenvironment, Provide growth matrix for injured tissue, can achieve the purpose of promoting tissue regeneration.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials and biomedical engineering, relates to tissue engineering, solid physics and bionic bioactive material preparation methods, in particular to magnetoelectric response bionic hydrogel and adjustable Cell electrical microenvironment magnetoelectric response biomimetic hydrogel and its preparation method. Background technique [0002] Electrical stimulation, as a means of physical stimulation, has been rapidly developed in regenerative medicine and tissue engineering. In recent years, the feasibility of using electrical stimulation to promote regeneration and differentiation of injured nerve tissue has been confirmed. Appropriate electrical stimulation can promote the growth, proliferation, and differentiation of nerve cells. However, invasive electrical stimulation often needs to face two problems: (I) the contact problem of the electrode / tissue interface; (II) exogenous electrical sti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08L89/00C08L5/08C08K3/22C08K3/24C08K9/10A61L27/02A61L27/20A61L27/24A61L27/38A61L27/50A61L27/52
CPCC08J3/075A61L27/52A61L27/50A61L27/025A61L27/24A61L27/20A61L27/383A61L27/3821A61L27/3804C08J2389/00C08J2405/08C08J2305/08C08K2003/2241C08K3/24C08K9/10A61L2430/32A61L2430/02A61L2400/12C08L5/08
Inventor 范红松张雨生陈素萍吴承恒卫丹孙静
Owner SICHUAN UNIV
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