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Bacillus clausii strain as well as screening method and application thereof

A technology of Bacillus clausii and Bacillus, applied in the field of microorganisms, can solve the problems of limited ability to secrete siderophore, need to improve the growth-promoting effect, and limit the growth-promoting ability, achieve good antibacterial effect, and the preparation method is simple and strong The effect of competition

Active Publication Date: 2021-11-09
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacillus clausii is a Gram-positive bacterium that widely exists in nature. The bacterium has the functions of biological control, biodegradation and plant growth promotion in the agricultural field. The growth-promoting mechanism of bio-control bacteria can be expressed by secreting Siderophores, nitrogen fixation, phosphorus solubilization, and indole acetic acid production can improve the utilization of nutrients by plants and enhance plant resistance to diseases, but their ability to secrete siderophores is limited, which limits their ability to promote growth
[0004]In recent years, the dosage forms of bio-control bacteria at home and abroad are mainly applied in the form of suspension concentrates and granules, which are not conducive to absorption, and the growth-promoting effect needs to be improved

Method used

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  • Bacillus clausii strain as well as screening method and application thereof
  • Bacillus clausii strain as well as screening method and application thereof
  • Bacillus clausii strain as well as screening method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0040] This example is the screening of Bacillus clausii Bcjcm062. 152 soil samples were collected from Heilongjiang Province, Jilin Province, Inner Mongolia Autonomous Region, Yunnan Province, Ningxia Hui Autonomous Region, Guizhou Province, and Jiangsu Province, and the two-line confrontation method was used to conduct a confrontation test with Fusarium verticillioide. A kind of Bacillus clausii was screened out, and its antibacterial distance to the target bacteria can reach 12mm.

[0041] The screening steps are as follows:

[0042] Step 1, the soil sample for testing is made into 10 -2 -10 -6 The soil suspension was multiplied by five gradients, spread evenly on the beef extract peptone medium (NA) plate by the plate coating method until it was completely absorbed, set three repetitions, and incubated at a constant temperature of 28°C for 48h.

[0043] Step 2: Pick single colonies with different colors and shapes, streak and purify them on NA medium for 3 times, and cu...

Embodiment 2

[0050] The present embodiment is the strain identification example of Bacillus clausii Bcjcm062:

[0051] 1. Strain activation: Streak the Bcjcm062 screened in Example 1 onto a PDA plate and grow in a constant temperature incubator at 28° C. for 1 day.

[0052] 2. Preparation of Bacillus clausii fermentation broth: pick a single colony of the strain and put it in a 5mL / 50mL LB liquid centrifuge tube, culture it in a shaker at 28°C and 180r / min for 18h, then inoculate it into 60mL / 250mLBcjcm062 with 7% inoculation amount for fermentation Culture medium in a triangular flask (fermentation medium: maltose 2v%, peptone 1.5v%, calcium chloride 1v%, the rest is distilled water, pH=5.5) at 31°C, 180r / min shaker for 48h, after 0.22 Filter with a μL membrane filter and store in a refrigerator at 4°C for later use.

[0053] The following tests and characterizations were performed on the screened Bacillus clausii Bcjcm062:

[0054] 1. The identification of Bacillus clausii Bcjcm062 has...

Embodiment 3

[0085] This embodiment is the preparation method of Bacillus clausii seed coating agent.

[0086] The bacillus clausii fermentation filtrate that embodiment 2 obtains and corn seed are fully mixed by mass ratio 1:50, add film-forming agent (film-forming agent and corn seed mass ratio are 1:100, and its active ingredient is resin and wax emulsion), to obtain Bcjcm062 seed coating agent.

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Abstract

The invention discloses a bacillus clausii strain as well as a screening method and application thereof. The bacillus clausii strain is obtained by performing a confrontation test on a two-line confrontation method and corn root rot fungi (fusarium verticillium). Compared with currently known bacillus clausii, the strain disclosed by the invention can generate two siderophores, namely a trihydroxy hydroxamic acid type siderophore and a catechol type siderophore, and can promote plant growth. The strain has a relatively good antibacterial effect on main pathogenic bacteria of corn diseases and has a broad-spectrum prevention and treatment effect.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a specific bacterial strain of Bacillus clausii and a screening method and application thereof. Background technique [0002] Corn is an important food crop in my country, and it is also an important source of feed and industrial raw materials. Its planting area and output rank first among food crops in my country. With the increasing demand for corn, it is necessary to ensure the production and quality of corn. Significant significance. However, the problem of soil compaction caused by long-term single planting of corn and frequent use of chemical fertilizers and chemicals has become more and more serious, resulting in more and more serious diseases such as corn root rot, sheath rot, and ear rot. Among them, root rot is the most common in my country. There are different degrees of occurrence in the main corn producing areas of China. The incidence rate is about...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12Q1/18A01C1/06A01C1/08A01N63/22A01P21/00A01P3/00C12R1/07
CPCC12N1/20C12N1/04C12Q1/18A01C1/06A01C1/08A01N63/22
Inventor 薛春生李玉蕾吴跃星肖淑芹崔金月
Owner SHENYANG AGRI UNIV
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