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Application of sulphated sea cucumber polysaccharide and weak acid degradation product thereof in promotion of lactobacillus proliferation

A technology of sulfated polysaccharides and degradation products, applied in the fields of application, polysaccharide/gum-containing food ingredients, food science, etc., can solve problems such as no effective solution, and achieve the goal of being conducive to recovery, promoting the formation of Lactobacillus biofilm, and promoting the proliferation of Lactobacillus Effect

Pending Publication Date: 2021-11-16
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no specific effective regimen for postoperative recovery from this treatment

Method used

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  • Application of sulphated sea cucumber polysaccharide and weak acid degradation product thereof in promotion of lactobacillus proliferation
  • Application of sulphated sea cucumber polysaccharide and weak acid degradation product thereof in promotion of lactobacillus proliferation
  • Application of sulphated sea cucumber polysaccharide and weak acid degradation product thereof in promotion of lactobacillus proliferation

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0044] A preparation method of sea cucumber sulfated polysaccharide, comprising the following steps:

[0045] S1. Boil the sea cucumber in water at 90-100°C until the protein is denatured, drain it, make it into blocks, freeze-dry it, soak it in acetone at 0-10°C for 12-48 hours, and dry it at room temperature to obtain a freeze-dried sample;

[0046] S2. Take the freeze-dried sample, add 0.1-0.3mol / L sodium acetate buffer solution with pH 6.0, 5-15% (w / w) papain, 2-6% (w / w) ethylenediaminetetra After mixing acetic acid and 1-5% (w / w) cysteine, shake the enzymolysis in a water bath at 50-60°C for 20-30 hours, centrifuge at 4000-8000g, 5-20min, and room temperature to obtain the supernatant;

[0047]S3. Add 5-15% (v / v) cetylpyridinium chloride solution to the supernatant, place it at room temperature for 12-48 hours, then centrifuge at 4000-8000g for 5-20min at room temperature Precipitate; dissolve the precipitate in 3mol / L NaCl-ethanol solution, then add 80-95% ethanol solut...

Embodiment 1

[0054] This example is used to prepare sea cucumber sulfated polysaccharide and its weak acid degradation products.

[0055] The specific method is as follows:

[0056] Sea cucumbers were washed, boiled, drained, cut into small pieces, and then freeze-dried to obtain samples. The freeze-dried samples were soaked in acetone at 4°C for 24h, and dried at room temperature. Taking 1.0g of freeze-dried sample as an example, add 30mL of 0.1mol / L sodium acetate buffer solution (pH6.0), 100mg of papain, 48mg of ethylenediaminetetraacetic acid and 18mg of cysteine, vortex to mix, and store at 60℃ Enzymolysis was carried out by shaking in a water bath for 24 hours, and the reaction mixture was centrifuged (6000 g, 15 min, room temperature) to obtain the supernatant. Add 1.6mL of 10% cetylpyridinium chloride solution to the supernatant, leave it at room temperature for 24h, centrifuge (8000g, 15min, room temperature) to get the precipitate. The precipitate was dissolved in 15 mL of 3 m...

Embodiment 2

[0059] This example is used to determine the composition of the weak acid degradation product of the sea cucumber sulfated polysaccharide prepared in Example 1.

[0060] The specific method is as follows:

[0061] The composition of oligosaccharides and monosaccharides in the weak acid degradation products of sea cucumber polysaccharides was detected by PMP derivatization method and high performance liquid chromatography-mass spectrometry. Take 5.0 mg of the sample, add 400 μL of ammonia water, and heat and stir 400 μL of PMP in a 70° C. water bath for 30 minutes. After centrifugal concentration to dryness, add 500 μL of methanol, and then centrifugal concentration to dryness, and repeat the operation twice. Add 1.0 mL of 1% acetic acid and 1.0 mL of chloroform, shake for 5 minutes, remove the chloroform, repeat the extraction three times, and use the water layer as the test solution. After diluting the test solution 10 times, pass through a 0.22 μm microporous membrane. Th...

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Abstract

The invention provides application of sulphated sea cucumber polysaccharide and a weak acid degradation product thereof in promotion of proliferation of lactobacillus, and is used for promoting proliferation of lactobacillus gasseri (Lactobacillus gasseri), lactobacillus johnsonii (Lactobacillus johnsonii) and lactobacillus reuteri (Lactobacillus reuteri) and forming a biological membrane.

Description

technical field [0001] The invention relates to the field of lactobacillus proliferation, more specifically, relates to the application of sea cucumber sulfate polysaccharide and its weak acid degradation product in promoting the proliferation of lactobacillus. Background technique [0002] Lactic acid bacteria have a variety of biological activities, including improving gastrointestinal function, maintaining intestinal microecological balance, improving immunity and lowering serum cholesterol. Lactic acid bacteria include Lactobacillus (Lactobacillus), Lactococcus (Lactococcus) and Streptococcus (Streptococcus), etc., wherein Lactobacillus is an important class of lactic acid bacteria. Lactobacillus accompanies the growth of human beings. The composition of Lactobacillus is single, mainly Lactobacillus gasseri (Lactobacillus gasseri) or Lactobacillus salivarius (Lactobacillus salivarius), and the Lactobacillus strains in adult feces are mainly rhamnose milk. Lactobacillus ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/125C08B37/00
CPCA23L33/125C08B37/0003A23V2002/00A23V2200/3202A23V2250/51
Inventor 宋爽刘正奇朱蓓薇艾春青温成荣林心萍邓安福
Owner DALIAN POLYTECHNIC UNIVERSITY
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