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Phosphorus solubilizing bacterium 3-1 and application thereof in production of plant growth hormone

A technology of plant growth hormone and phosphate-solubilizing bacteria, applied in the field of phosphate-solubilizing bacteria, can solve the problems of acidification fertility, soil pollution, soil compaction, etc., and achieve the effects of reducing the use of chemical fertilizers, alleviating soil compaction, and enhancing soil fertility

Active Publication Date: 2021-11-16
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term input of phosphate fertilizers causes soil compaction, acidification and fertility decline, leading to serious water pollution and soil pollution

Method used

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  • Phosphorus solubilizing bacterium 3-1 and application thereof in production of plant growth hormone
  • Phosphorus solubilizing bacterium 3-1 and application thereof in production of plant growth hormone
  • Phosphorus solubilizing bacterium 3-1 and application thereof in production of plant growth hormone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Isolation and Screening of Phosphobacteria 3-1

[0033] Soil samples were collected from the phosphate mine in Yichang City, Hubei Province (N: 31°13’55”N, E: 111°15’36”), put them into sterile bags and brought them back to the laboratory, and stored them at 4°C. Weigh 10 g of fresh soil samples and add them to 90 mL of sterile saline, shake at 100 r / min for 15 min, and place them in an incubator at 28 °C for 24 h. After enrichment, the supernatant was diluted to 10 -3 、10 -4 、10 -5 For the three gradients, 350 μL of each dilution was applied to the inorganic phosphorus screening solid medium, with 3 parallels in each group. Place it upside down in a 28°C incubator for 4-5 days, and observe the transparent circle. Pick the colony with a transparent circle and repeatedly streak and purify it. After purification, pick a single colony and transfer it to the slant of beef extract peptone medium and store it at 4°C for later use.

[0034] Preserved by China Center for T...

Embodiment 2

[0036] Determination of the ability to produce IAA

[0037] Prepare 50mL liquid King's (King) culture based on 150mL Erlenmeyer flask, inoculate 500μL Phospholyticum 3-1 bacterial suspension resuspended with sterile water after sterilization (1×10 8 cfu / mL), three replicates were set up, and the uninoculated medium was used as the control. The culture solution was placed in a shaker at 28°C at 200r / min for 12 days. Measured by S2 colorimetric method, centrifuge the 12-day culture medium at 10000r / min at 4°C for 10min, take 1mL of supernatant and mix with 1mL of S2 colorimetric solution evenly, keep it in the dark for 30min, and measure it with a spectrophotometer The absorbance value at 530nm was used to calculate the IAA yield with the standard curve.

[0038] Preparation of IAA standard curve: Accurately weigh 2.8mg of IAA in a 100mL volumetric flask, and dilute to volume with methanol. Dilute with methanol to different concentrations, respectively 0mg / L, 4mg / L, 8mg / L, 12...

Embodiment 3

[0043] gibberellin-producing ability assay

[0044] Pick the activated Phosphobacteria 3-1 strain and place it in NB liquid medium, shake it overnight at 28°C to make a seed liquid, add 100 μL of seed liquid to the inorganic phosphorus screening liquid medium ①, and set the temperature at 28°C at 200r / Min shaking culture 5d, every 24h to take 0.5mL test solution to measure the absorbance at 412nm.

[0045] Preparation of the gibberellin standard curve: Weigh the analytically pure gibberellin and dissolve it in ethanol with a mass concentration of 70%, and prepare a gibberellin content standard solution with a concentration of 100 μg / mL, and follow the concentration gradient of 0, 10, 20, 30, 40, 50μg / mL, take 0.5mL of gibberellin of each concentration and mix with 4.5mL of concentrated sulfuric acid, set the volume to 20mL and measure its absorbance at 412nm, take the concentration of gibberellin as the abscissa, and the The absorbance value is the ordinate, and a standard c...

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Abstract

The invention relates to the technical field of agriculture and microorganisms, and discloses a phosphate solubilizing bacterium 3-1 with high salt resistance and heavy metal cadmium resistance and application thereof. The bacterial strain is Enterobacter ludwigii strain 3-1 which is preserved in the China Center for Type Culture Collection (CCTCC) of Wuhan University on June 18, 2021, and the preservation number is CCTCC M 2021734. The phosphate solubilizing bacterium 3-1 disclosed by the invention has the effects of solubilizing phosphorus, fixing nitrogen, producing plant growth hormone and promoting plant growth, and can be applied to soil with high salinity and high heavy metal concentration. When the phosphate solubilizing bacterium 3-1 is applied to soil and plant seedlings, the content of available phosphorus in the soil can be remarkably increased, the plant height, wet weight, dry weight and rhizome length of plants are greatly increased, and the phosphorus-containing compound fertilizer has important significance on agricultural production and sustainable development.

Description

technical field [0001] The invention relates to the fields of agricultural planting and microbiology, in particular to a phosphate solubilizing bacterium and its application. Background technique [0002] Phosphorus is the most important nutrient limiting plant growth after nitrogen. However, about 74% of the cultivated land in my country is deficient in phosphorus, and the available inorganic phosphorus available to plants only accounts for 2% to 3% of the total phosphorus in the soil. To ensure agricultural production, a large amount of phosphorus fertilizer is needed. However, studies have shown that the seasonal utilization rate of phosphorus fertilizers is only 10% to 20%, and the total utilization rate does not exceed 25%. At present, my country's farmland agricultural soil surplus has reached 282×10 4 t. Long-term input of phosphate fertilizers causes soil compaction, acidification and fertility decline, leading to serious water pollution and soil pollution. There...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P21/00C09K17/14C12P17/10C12P27/00C09K101/00C09K109/00C12R1/01
CPCA01N63/20C09K17/14C12P17/10C12P27/00C09K2101/00C09K2109/00
Inventor 郭金玲孙语健方成龚大春吕育财任立伟杨潇
Owner CHINA THREE GORGES UNIV
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