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A kind of tissue culture method of semi-mangrove hibiscus hibiscus

A technology of tissue culture and mangrove plants, applied in the field of plant tissue culture, can solve the problems of being easily affected by seasons, high demand, survival rate, and discarded twigs

Active Publication Date: 2022-05-10
TAIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, hibiscus is mainly cultivated by sowing and cutting. Seeds need to be collected, dried and screened for good seeds. Cuttings usually use semi-lignified branches, and the twigs are often discarded, which is not friendly to the environment; in addition, cuttings are generally carried out from March to June, which is easily affected by seasons
Hibiscus hibiscus is used as a protective plant, which is in great demand and requires a relatively high survival rate, but so far, there are very few reports on tissue culture techniques for hibiscus hibiscus

Method used

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  • A kind of tissue culture method of semi-mangrove hibiscus hibiscus
  • A kind of tissue culture method of semi-mangrove hibiscus hibiscus
  • A kind of tissue culture method of semi-mangrove hibiscus hibiscus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The tissue culture method of embodiment 1 hibiscus hibiscus

[0033] (1) Selection and disinfection of explants: take the young and tender stem tips of the hibiscus japonica as explants, wash them with tap water, put them in 75% alcohol for 3 seconds, wash them with sterile water for 3 times, and then wash them with 0.1% HgCl 2 Soak and sterilize for 3 minutes, then rinse with sterile water 5 times.

[0034] (2) Callus formation: insert the sterilized Hibiscus japonica stem tip into the induction medium, and culture it in the dark at 23°C for 3 days to induce the generation of callus tissue, then place it in 1500Lux light culture for 12h and dark culture for 12h alternately, Co-culture for 14 days; the induction medium is 2 / 3MS medium+1.5mg / L 6-BA+0.15mg / L NAA+25g / L sucrose+4.5g / L agar, pH6.0;

[0035] (3) Cluster bud induction formation: transfer the obtained callus to the differentiation medium, at 23°C, 1500Lux light culture for 12h and dark culture for 12h alternat...

Embodiment 2

[0039] The tissue culture method of embodiment 2 hibiscus hibiscus

[0040](1) Selection and disinfection of explants: take the young and tender stem tips of the hibiscus japonica as explants, wash them with tap water, put them in 75% alcohol for 7 seconds, wash them with sterile water for 3 times, and then wash them with 0.1% HgCl 2 Soak and sterilize for 4 minutes, then rinse with sterile water 6 times.

[0041] (2) Callus formation: insert the sterilized stem tip of Hibiscus hibiscus into the induction medium, culture it in the dark at 24°C for 4 days, then place it in 2300Lux light culture for 12 hours and dark culture for 12 hours alternately, co-cultivate for 19 days, and induce Generate callus; the induction medium is 2 / 3MS medium+2.0mg / L 6-BA+0.20mg / L NAA+30g / L sucrose+6.5g / L agar, pH6.0;

[0042] (3) Cluster bud induction formation: the obtained callus is transferred to the differentiation medium, at 24°C, 2300 Lux light culture for 12h and dark culture for 12h are c...

Embodiment 3

[0046] The tissue culture method of embodiment 3 hibiscus hibiscus

[0047] (1) Selection and disinfection of explants: take the young and tender stem tips of the hibiscus japonica as explants, wash them with tap water, put them in 75% alcohol for 12 seconds, wash them with sterile water for 5 times, and then wash them with 0.1% HgCl 2 Soak and sterilize for 5 minutes, then rinse with sterile water 7 times. .

[0048] (2) Callus formation: Insert the sterilized stem tip of Hibiscus hibiscus into the induction medium, culture it in the dark at 25°C for 5 days, then place it in 3000Lux light culture for 12 hours and dark culture for 12 hours alternately, co-cultivate for 21 days, and induce Generate callus; the induction medium is 2 / 3MS medium+3.0mg / L 6-BA+0.30mg / L NAA+32g / L sucrose+8.5g / L agar, pH6.0;

[0049] (3) Cluster bud induction formation: transfer the obtained callus to the differentiation medium, at 25°C, 3000Lux light culture for 12h and dark culture for 12h alterna...

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PUM

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Abstract

The invention discloses a method for tissue culture of the semi-mangrove hibiscus hibiscus, belonging to the field of plant tissue culture. The method comprises: (1) selection and disinfection of explants: taking the tender shoot tips of Hibiscus japonica as explants, and disinfecting them; (2) Formation of callus: inserting the shoot tips of sterilized Hibiscus japonica into induced culture (3) clustered shoot induction: the callus was transferred to the differentiation medium to induce the formation of clustered shoots; (4) clustered shoot subculture: clustered shoots were induced to an average height of 1 When -2cm, transfer the single clustered buds to the subculture medium, and cultivate to an average height of 3-4cm; (5) root induction formation: the mature buds after subculture are transferred to the rooting medium, and the roots are induced Differentiate into tissue culture seedlings; (6) Seedling plant formation. The invention can rapidly obtain a large amount of hibiscus hibiscus tissue culture seedlings through tissue culture, the survival rate is higher than 98.5%, and can meet the demand for afforestation of shelterbelts in coastal areas.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture of the semi-mangrove hibiscus hibiscus. Background technique [0002] Yellow hibiscus (scientific name: Hibiscus tiliaceus Linn.) is an evergreen shrub or tree of Malvaceae and Hibiscus, distributed in tropical countries such as China, Vietnam, Cambodia, Laos, Myanmar, India, Indonesia, Malaysia and the Philippines; distributed in China Taiwan, Guangdong and Fujian provinces. It is mainly for viewing leaves and flowers. The flowering period of the yellow hibiscus is year-round, and it is the most prosperous in summer. It can be planted for street trees and coastal landscaping. Mostly grown in coastal areas, it is an excellent semi-mangrove tree species for coastal sand, moisture, and wind protection. [0003] At present, hibiscus is mainly cultivated by sowing and cutting. Sowing requires seed collection, drying, and screening of high-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G24/28A01G24/10
CPCA01H4/002A01H4/008A01G24/28A01G24/10Y02A40/22Y02P60/40
Inventor 杜照奎杨党李钧敏
Owner TAIZHOU UNIV
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