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Method for promoting P.stutzeri aerobic denitrification by adopting B. licheniformis

An aerobic denitrification and system technology, applied in chemical instruments and methods, aerobic and anaerobic process treatment, water pollutants, etc., can solve the problems of slow aerobic denitrification denitrification speed, etc., to improve total nitrogen removal Efficiency, promotion of aerobic denitrification, and improvement of membrane transport rate

Active Publication Date: 2021-11-26
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose is to solve the problem of slow aerobic denitrification denitrification
Proposed to use B.licheniformis to promote the membrane transport ability of aerobic denitrifying microorganism P.stutzeri to solve the problem

Method used

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  • Method for promoting P.stutzeri aerobic denitrification by adopting B. licheniformis
  • Method for promoting P.stutzeri aerobic denitrification by adopting B. licheniformis
  • Method for promoting P.stutzeri aerobic denitrification by adopting B. licheniformis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The denitrifying microorganisms P. stutzeri and B. licheniformis were cultured with sterilized LB under aerobic conditions in advance to OD 600 Both are 2; the construction of the aerobic denitrification system is carried out in the Erlenmeyer flask, and the medium composition of the aerobic denitrification system is: 8.11mM KNO 3 , 10.66mM NH 4 Cl, 0.41mM MgSO 4 ·7H 2 O, 17.93 mM KH 2 PO 4 , 32.76mM Na 2 HPO 4 , trace element 1mL; trace element composition (g / L): Na 2 -EDTA(7.30), FeSO 4 ·7H 2 O(2.50), MnCl 2 4H 2 O(0.02), Na 2 MoO 4 2H 2 O(0.242), CuCl 2 2H 2 O(0.135) and ZnCl2 (0.34); in a sterile Erlenmeyer flask containing 100mL of aerobic denitrification system medium, adjust the pH of the system to 7.2 with NaOH and HCl, seal it with gauze, and sterilize at 121°C for 15min; after cooling, add and filter carbon source C 6 h 12 o 6 , set the carbon-nitrogen ratio to 10;

[0026] Inoculate 1 mL of pre-cultured denitrifying microorganism P. stutze...

Embodiment 2

[0028] The denitrifying microorganisms P. stutzeri and B. licheniformis were cultured with sterilized LB under aerobic conditions in advance to OD 600 Both are 2; the construction of the aerobic denitrification system is carried out in the Erlenmeyer flask, and the medium composition of the aerobic denitrification system is: 8.11mM KNO 3 , 10.66mM NH 4 Cl, 0.41mM MgSO 4 ·7H 2 O, 17.93 mM KH 2 PO 4 , 32.76mM Na 2 HPO 4 , trace element 1mL; trace element composition (g / L): Na 2 -EDTA(7.30), FeSO 4 ·7H 2 O(2.50), MnCl 2 4H 2 O(0.02), Na 2 MoO 4 2H 2 O(0.242), CuCl 2 2H 2 O(0.135) and ZnCl 2 (0.34); in a sterile Erlenmeyer flask containing 100mL of aerobic denitrification system medium, adjust the pH of the system to 7.2 with NaOH and HCl, seal it with gauze, and sterilize at 121°C for 15min; after cooling, add and filter carbon source C 6 h 12 o 6 , set the carbon-nitrogen ratio to 5;

[0029] Inoculate 1 mL of pre-cultured denitrifying microorganism P. stutz...

Embodiment 3

[0031] The denitrifying microorganisms P. stutzeri and B. licheniformis were cultured with sterilized LB under aerobic conditions in advance to OD 600 Both are 2; the construction of the aerobic denitrification system is carried out in the Erlenmeyer flask, and the medium composition of the aerobic denitrification system is: 8.11mM KNO 3 , 10.66mM NH 4 Cl, 0.41mM MgSO 4 ·7H 2 O, 17.93 mM KH 2 PO 4 , 32.76mM Na 2 HPO 4 , trace element 1mL; trace element composition (g / L): Na 2 -EDTA(7.30), FeSO 4 ·7H 2 O(2.50), MnCl 2 4H 2 O(0.02), Na 2 MoO 4 2H 2 O(0.242), CuCl 2 2H 2 O(0.135) and ZnCl 2 (0.34); in a sterile Erlenmeyer flask containing 100mL of aerobic denitrification system medium, adjust the pH of the system to 7.2 with NaOH and HCl, seal it with gauze, and sterilize at 121°C for 15min; after cooling, add and filter carbon source C 6 h 12 o 6 , set the carbon-nitrogen ratio to 10;

[0032] Inoculate 1 mL of pre-cultured denitrifying microorganism P. stut...

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Abstract

The invention provides a method for promoting aerobic denitrification of P.stutzeri by adopting B. licheniformis. The method is realized by inoculating the B. licheniformis into a denitrification system of the aerobic denitrification microorganism P.stutzeri according to an inoculum size ratio of (1: 0.1)-(1: 5.0), an electron donor and an electron acceptor in the system are respectively glucose and nitrate, and an aerobic bottle is placed in a shaking table at 20-35 DEG C for culture. According to the method, nitrate is removed under the condition that the carbon nitrogen ratio of the culture medium is 1-15 and the dissolved oxygen is 4.0-8.0 O2 mg / L. According to the method disclosed by the invention, the membrane transport capacity of P.stutzeri is improved by utilizing the B.licheniformis to promote aerobic denitrification nitrogen removal, so that the cost is low, secondary pollution is avoided, the nitrate removal effect is obviously improved, the accumulation of nitrite and nitrous oxide is reduced, and the total nitrogen removal efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a method for promoting P. stutzeri aerobic denitrification by adopting B. licheniformis. Background technique [0002] The traditional view is that nitrification and denitrification cannot occur at the same time because of the different requirements for molecular oxygen, which makes it necessary to set up nitrification tanks and denitrification tanks separately in the wastewater denitrification process. The discovery of aerobic denitrification broke through this point of view, making simultaneous nitrification and denitrification, that is, nitrification, denitrification and COD removal possible in the same reactor. The advantage of synchronous nitrification and denitrification is that it can effectively maintain the stability of the pH value in the reactor, without the need for acid-base neutralization, reduce the external carbon source, save the volu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/30C02F3/34C02F101/16
CPCC02F3/34C02F3/305C02F3/303C02F3/301C02F2101/163Y02W10/10
Inventor 陈守文蒋萌蔡冬波李庆熙何鹏辉胡施颖吴雅婷
Owner HUBEI UNIV