Double-layer guide bone regeneration stent and preparation method thereof
A guided bone regeneration, double-layer technology, applied in bone implants, pharmaceutical formulations, medical science, etc., can solve the problem of unfavorable nutrient diffusion, cell adhesion and growth, limit cell interaction, and fail to prepare three-dimensional scaffolds Structure and other issues, to meet the requirements of mechanical strength and degradation rate, dense fiber arrangement, and control inflammation
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Embodiment 1
[0088] A double-layer guided bone regeneration scaffold, comprising a loose layer 1 and a dense layer 2, the dense layer 2 is arranged on the loose layer 1, the porosity of the loose layer 1 is 96%, and the pores of the dense layer rate of 55%, the thickness of the loose layer 1 is 0.41mm, the thickness of the dense layer 2 is 0.19mm, that is, the thickness ratio of the loose layer 1 and the dense layer 2 is 2:1, the loose layer 1 includes several first loose parts 11 and second loose parts 12 arranged alternately, the first loose parts 11 include several parallel first support units 111, and the second loose parts 12 include several parallel second support units 121, the first support unit 111 intersects with the second support unit 121 to form micropores, the first support units 111 of different first loose parts are arranged in parallel, and the second support units 121 of different second loose parts are arranged in parallel cloth, the distance between adjacent first suppo...
Embodiment 2
[0094] The morphology of the double-layer guided bone regeneration scaffold prepared by near-field direct-writing printing combined with electrospinning technology in the above-mentioned Example 1 was characterized, and the results were as follows: image 3 shown. The general photographs of the loose layer and the dense layer show that the structure of the fiber scaffold in the loose layer is regular, the fibers are interwoven at 90°, and the pores between the fibers are large and clear ( image 3 A); the fiber structure of the dense layer is dense, and the fiber arrangement direction is irregular ( image 3 B). Scanning electron microscopy results show that the structure of the loose layer is regular, the diameter of a single fiber is 10.2±0.5 μm, the fibers are stacked in a 90° interweaving manner, and the distance between the fibers is 400 μm. Through the loose layer, it can be seen that the fibers at the bottom are tightly packed. Interwoven dense layers ( Figure 4 A)....
Embodiment 3
[0096] The degradation performance of the double-layer guided bone regeneration scaffold prepared by near-field direct-writing printing combined with electrospinning technology in the above-mentioned Example 1 was characterized. The prepared fiber scaffold was cut into a square of 10mm×10mm. Before the measurement starts, each film is accurately weighed, and the mass is recorded as m 0 . Subsequently, each sample was placed in a centrifuge tube containing 5mL PBS (pH 7.4) or 5mL artificial saliva (pH 7.4), and then the centrifuge tube was incubated in a constant temperature shaker at 37°C with a shaking speed of 100rpm. When the predetermined time point is reached, the residual salt or enzyme solution on the surface of the fiber membrane is thoroughly cleaned with deionized water, then freeze-dried and weighed, and the recorded mass is m 1 . Then the degradation percentage of each sample at each time point can be calculated according to the following formula: degradation pe...
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