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Clenbuterol enantiomer resolution and determination method based on ultra performance convergence chromatography technology

A technique for efficient convergence chromatography and enantiomeric separation, applied in the field of high-efficiency detection of clenbuterol

Pending Publication Date: 2021-12-14
ZHEJIANG ACAD OF SCI & TECH FOR INSPECTION & QUARANTINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently, UPC 2 The application of technology to the separation and content determination of clenbuterol enantiomers is rarely reported

Method used

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  • Clenbuterol enantiomer resolution and determination method based on ultra performance convergence chromatography technology
  • Clenbuterol enantiomer resolution and determination method based on ultra performance convergence chromatography technology
  • Clenbuterol enantiomer resolution and determination method based on ultra performance convergence chromatography technology

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] 1 Experimental part

[0025] 1.1 Instruments, materials and reagents

[0026] Acquity ultra-high efficiency convergence chromatograph (U.S. Waters company, with PDA diode array detector); AE260 electronic balance (Swiss Mettler company); R215 rotary evaporator (Switzerland Buchi company); ELGA CLXXXUVM2 ultrapure water purification system (UK Elga company); MS2 vortex mixer (Shanghai Medical University Instrument Factory); N-EVAP TM 111 Nitrogen Blowing Apparatus (Tokyo Physical and Chemical Company, Japan).

[0027] Acetonitrile, methanol, formic acid, ethanol, isopropanol, n-heptane (chromatographically pure, Scharlau, Spain); ammonium acetate, ammonia water (excellent pure); water is ultrapure water; high-purity carbon dioxide (99.999%); other experiments All reagents used were of analytical grade unless otherwise specified.

[0028]Chiral separation column: Acquity Trefoil CEL2 (3.0mm×150mm, 2.5μm, filled with cellulose-tris(3-chloro-4-methylphenylcarbamate)), A...

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Abstract

The invention belongs to the technical field of analytical chemistry, and relates to an efficient detection method of clenbuterol, in particular, a clenbuterol enantiomer resolution and determination method based on an ultra performance convergence chromatography technology. According to the clenbuterol enantiomer resolution and determination method based on the ultra performance convergence chromatography technology, clenbuterol enantiomers are (+)-clenbuterol enantiomers and (-)-clenbuterol enantiomers. The analysis conditions of the method are as follows: an Acquity Trefol AMY1 chiral chromatographic column is adopted, a CO2-0. 5% (v / v) ammonium acetate methanol solution is used as a mobile phase, gradient elution is performed, the flow rate is 2.0 mL / min, the detection wavelength is 241 nm, the sample introduction volume is 10 microlitre, the column temperature is 40 DEG C, and the system back pressure is 13.8 MPa. The method has the characteristics of high analysis speed, good separation effect, low organic solvent consumption and the like, and has important significance for further researching the pharmacology of clenbuterol and developing new drugs with smaller side effects.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, in particular to a high-efficiency detection method for clenbuterol. Background technique [0002] Clenbuterol, chemical name 1-(4-amino-3,5-dichlorophenyl)-2-(tert-butyl-D9-amino)ethanol, is a selective beta 2 -Adrenergic receptor agonists, mainly used as bronchial antispasmodic drugs clinically, have good effects on bronchial asthma and chronic bronchitis with reversible airway obstruction [1] . clinically used beta 2 The adrenergic agonist drug is the racemate of clenbuterol [2] , which contains one chiral carbon atom in its molecule [3] , there is a pair of enantiomers, including (+)-clenbuterol enantiomer and (-)-clenbuterol enantiomer, two enantiomeric structural formulas such as figure 1 shown. According to literature reports, different structural enantiomers will produce different pharmacological activities [4,5] . Studies have found that the biological activities of ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 张文华徐敦明侯建波刘海山谢文黄超群裘慧严颖鹏李可郗存显
Owner ZHEJIANG ACAD OF SCI & TECH FOR INSPECTION & QUARANTINE
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