Preparation method of high-purity sennoside and sennoside or derivative prepared by same

Abstract

The invention discloses a preparation method of high-purity sennoside and sennoside or derivatives prepared by the preparation method. The preparation method comprises the following steps: (1) extracting medicinal materials; (2) ion exchange column chromatography: balancing an ion exchange column by using equilibrium liquid A which is 3-5 times of the column volume; loading the acid-regulated supernate obtained in the step (1) onto a chromatographic column, rebalancing by using equilibrium liquid A with the volume 3-5 times that of the column, and eluting by using eluent B with the volume 3-5 times that of the column to obtain eluent; (3) macroporous adsorption column chromatography: adjusting the pH value of the eluent obtained in the step (2) to 3.5-8.0, then loading the eluent to a macroporous adsorption chromatographic column, and eluting with an eluent E with the volume 3-5 times that of the column to obtain the eluent; and (4) carrying out low-temperature precipitation and solid-liquid separation on the eluent obtained in the step (3) to obtain an extract of the sennoside A / B compound. The sennoside is purified by using the macroporous adsorption chromatographic column and the ion exchange column chromatography, and the obtained sennoside extract is high in purity, low in cost and more environment-friendly.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a method for preparing high-purity sennoside and the prepared sennoside or derivative thereof. Background technique [0002] The main active ingredients in Folium sennae are anthraquinone derivatives. Among the anthraquinone derivatives, the dianthrone derivatives sennosides A, B, C, and D have stronger laxative effect and irritation than other anthraquinone-containing laxatives, and have little toxicity when taken orally, so they are commonly used in clinic For acute constipation. Dianthrone compounds are formed by connecting two molecules of anthrone C-C, wherein sennosides A and B, sennosides C and D are stereoisomers of each other. Sennosides have inhibitory effects on Streptococcus pneumoniae, Klebsiella pneumoniae and Staphylococcus aureus. In addition, sennosides also have insulin-sensitizing and α-glucoamylase inhibitory effects. Gu Yan et al. used macroporou...

AI Technical Summary

Problems solved by technology

Gu Yan et al. used macroporous resins to purify sennosides. The content of sennosides prepared by this method reached 50%, and the content of total sennosides reached more than 50%. Degradation, and due to the poor stability of sennoside, it is easy to decompose when exposed to heat and light, and transform into monoanthrone components. It is difficult to efficiently extract this component by conventional extraction and macroporous adsorption chromatography.

Taking the content of sennoside A as an index, Zhang Yang et al. studied the process conditions for extracting dianthrone in senna leaves, and obtained a product with a total sennoside content of 47.2%, with a yield of 2.93%. However, this method uses the organic solvent n-butyl Alcohol and ethanol, high cost and not environmentally friendly

Patent CN105254689A discloses a sennoside A.B salt compound and its preparation method and application. Senna leaves are used as raw materials to obtain a high-purity active ingredient sennoside A.B salt compound with a purity of 80% to 90%. , the same steps of this method are relatively cumbersome, and the last step of purification process uses acetone, methanol and other highly toxic organic solvents

[0003] In the prior art, senna traditional Chinese medicine is usually taken in the form of decoction or granules, and the decoction of the decoction is cumbersome, backward, and the dosage is large, and it cannot play a first aid role.

Current sennoside purification methods do not meet existing requirements

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