A kind of rapid detection method of cyanide content in nuts

A detection method and cyanide technology, which are applied in material analysis, measuring device, and preparation of test samples by observing the influence of chemical indicators, can solve the problems of high cost, difficult batch operation, and complicated processing, and achieve The effect of low equipment requirements, improved analysis efficiency, and simple operation

Active Publication Date: 2022-08-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Existing methods for detecting cyanide in nuts are complex, costly and difficult to operate in batches

Method used

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  • A kind of rapid detection method of cyanide content in nuts
  • A kind of rapid detection method of cyanide content in nuts
  • A kind of rapid detection method of cyanide content in nuts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 utilizes QuEChERS pretreatment technology to process nuts

[0046] After grinding and crushing the nut sample, pass it through a 425 μm standard sieve. Accurately weigh 5 g of nut powder into a 50 mL plastic centrifuge tube, add 10 mL of water, vortex and mix, soak for 30 min, add 15 mL of acetonitrile, sonicate for 15 min, freeze at -20°C for 8 min, and put in a ceramic homogeneous, add 6.0g MgSO 4 , 1.5g NaOAc, immediately shake and vortex for 1min, then centrifuge at 5000r / min for 5min, pipette (8mL) the supernatant into the liquid containing 1200mg MgSO 4 , 400mg C 18 and 400mg GCB in a 15mL plastic centrifuge tube, vortex for 1min, centrifuge at 5000r / min for 5min, pipette 1mL of supernatant into a 5mL EP tube, blow nitrogen to near dryness, and add 5mL of 2g / L NaOH for colorimetric spectroscopic analysis.

Embodiment 2

[0047] Embodiment 2 utilizes QuEChERS pretreatment technology to process nuts

[0048] The nut samples were ground and crushed and passed through a 425 μm standard sieve. Accurately weigh 2 g of nut powder into a 50 mL plastic centrifuge tube, add 4 mL of water, vortex and mix, soak for 15 min, add 6 mL of acetonitrile, sonicate for 8 min, freeze at -20°C for 8 min, and put in a ceramic homogeneous, add 2.4g MgSO 4 , 0.6g NaOAc, immediately shake and vortex for 1min, then centrifuge at 5000r / min for 5min, pipette (2mL) the supernatant to the solution containing 300mg MgSO 4 , 100mg C 18 and 100mg GCB in a 15mL plastic centrifuge tube, vortex for 1min, centrifuge at 5000r / min for 5min, pipette 1mL of supernatant into a 5mL EP tube, blow with nitrogen to near dryness, and add 5mL of 2g / L NaOH for colorimetric spectroscopic analysis.

Embodiment 3

[0049] The detection method of cyanide content in the fruit of embodiment 3

[0050] (1) Solution preparation: use the standard material GBW(E)080115 for the analysis of cyanide in water to prepare a standard intermediate solution of cyanide ions (1μg / mL); sodium hydroxide solution (2g / L, 20g / L); phenolphthalein solution (10g / L) L); acetic acid solution (1+24): mix acetic acid and water at a volume ratio of 1:24; chloramine T solution (10 g / L): weigh 1 g of chloramine T, dissolve in water, and dilute to 100 mL, Prepare for immediate use; Phosphate buffer (0.5M, pH=7); Isonicotinic acid-barbituric acid reagent: weigh 2.5 g isonicotinic acid (C 5 H 5 O 2 N) and 1.25g barbituric acid (C 4 H 4 N 2 O 3 ), add it to 100mL of sodium hydroxide (20g / L) solution at 60℃~70℃, stir until dissolved, add water to 100mL after cooling, and store in 4℃ refrigerator.

[0051] (2) Pretreatment of raw material QuEChERS: Nut samples were ground and pulverized and passed through a 425 μm stan...

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Abstract

The invention discloses a rapid detection method for cyanide content in nuts, and belongs to the technical field of food analysis and detection. The invention adopts the optimization of the formula of the buffer salt system and the extractant and the adsorbent for the pretreatment of QuEChERS, combined with the detection by the spectrophotometry, reacts with the chloramine T, CN ‑ It is converted into cyanogen chloride, and then reacts with isonicotinic acid-barbituric acid to generate a violet-blue dye. The absorbance is measured by a spectrophotometer, and the cyanide content in the sample is quantified by combining with the standard curve method. The detection method established by the invention can realize the rapid batch pretreatment of samples with low sample volume, has low requirements on instruments and equipment, can effectively improve the detection efficiency, and has the advantages of rapidity, economy and wide applicability.

Description

technical field [0001] The invention relates to a rapid detection method for cyanide content in nuts, and belongs to the technical field of food analysis and detection. Background technique [0002] Cyanide is a common class of highly toxic compounds. Among all cyanides, hydrogen cyanide is the most toxic and can enter the human body through oral administration, inhalation, injection, and absorption through skin and mucous membranes. EN - It can inhibit the activity of cytochrome C oxidase, block the respiratory chain and cause tissue hypoxia, which in turn produces a large amount of acidic substances such as lactic acid, which eventually leads to metabolic acidosis and causes a series of nervous system diseases in the human body. [0003] The current commercial value of nuts in the food, cosmetic and pharmaceutical fields is increasing. However, cyanogenic glycosides, which can be hydrolyzed to produce highly toxic substances in nuts, are an important hidden danger to the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N21/78G01N1/28
CPCG01N21/31G01N21/78G01N1/286G01N2001/2866
Inventor 陈双徐岩朱晓春
Owner JIANGNAN UNIV
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