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ROS responsive nano assembly diagnosis and treatment agent as well as preparation method and application thereof

A nano-assembly, responsive technology, applied in nanotechnology, nano-optics, nanotechnology, etc., to achieve good microenvironment responsiveness, good biosafety and clinical translation prospects, and mild reaction conditions.

Active Publication Date: 2022-01-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is necessary to develop a new diagnosis and treatment platform technology for acute liver injury in order to further standardize and improve the clinical diagnosis and treatment level of liver injury diseases in my country in view of the fact that the existing treatment and diagnosis methods are difficult to fully meet the needs of clinical treatment and patients.

Method used

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  • ROS responsive nano assembly diagnosis and treatment agent as well as preparation method and application thereof
  • ROS responsive nano assembly diagnosis and treatment agent as well as preparation method and application thereof
  • ROS responsive nano assembly diagnosis and treatment agent as well as preparation method and application thereof

Examples

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Embodiment 1

[0035] (1) Synthesis of ultra-small cerium oxide nanozyme: 0.4g cerium acetate hydrate and 3.2g oleylamine were added in 15ml xylene, stirred at room temperature for 12 hours, and rose to 90 degrees Celsius at a heating rate of 2 degrees Celsius per minute; 1ml of deionized water was injected into the reaction system protected by an inert gas, aged for three hours, precipitated with anhydrous ether, and centrifuged to obtain ultra-small cerium oxide nanocrystals.

[0036] (2) Synthesis of ROS-responsive amphiphilic block polymers: the small molecule diselenodiol and 2,4-cresyl diisocyanate were dissolved in tetrahydrofuran and stirred at 50°C for 24 hours under nitrogen protection. Further, polyethylene glycol monomethyl ether with a molecular weight of 400 was dissolved in tetrahydrofuran and added dropwise to the above reaction solution to continue the reaction for 48 hours. In the above reaction, the molar ratio of small molecule diselenodiol, 2,4-diisocyanate toluene and p...

Embodiment 2

[0039] (1) Synthesis of ultra-small cerium oxide nanozyme: 0.4g cerium acetate hydrate and 3.2g oleylamine were added in 15ml xylene, stirred at room temperature for 12 hours, and rose to 90 degrees Celsius at a heating rate of 2 degrees Celsius per minute; 1ml of deionized water was injected into the inert gas protection reaction system, aged for three hours, acetone precipitated, and centrifuged to obtain ultra-small cerium oxide nanocrystals.

[0040](2) Synthesis of ROS-responsive amphiphilic block polymers: small molecule dithiol and 2,4-cresyl diisocyanate were dissolved in tetrahydrofuran and stirred at 50°C for 24 hours under nitrogen protection. Further, polyethylene glycol monomethyl ether with a molecular weight of 1000 was dissolved in tetrahydrofuran and added dropwise to the above reaction solution to continue the reaction for 48 hours. In the above reaction, the molar ratio of small molecule dithiol, 2,4-diisocyanate toluene and polyethylene glycol monomethyl et...

Embodiment 3

[0043] (1) Synthesis of ultra-small cerium oxide nanozyme: 0.4g cerium acetate hydrate and 3.2g oleylamine were added in 15ml xylene, stirred at room temperature for 12 hours, and rose to 90 degrees Celsius at a heating rate of 2 degrees Celsius per minute; 1ml of deionized water was injected into the reaction system protected by an inert gas, aged for three hours, precipitated with anhydrous ether, and centrifuged to obtain ultra-small cerium oxide nanocrystals.

[0044] (2) Synthesis of ROS-responsive amphiphilic block polymers: the small molecule diselenodiol and 2,4-cresyl diisocyanate were dissolved in tetrahydrofuran and stirred at 50°C for 24 hours under nitrogen protection. Further, polyethylene glycol monomethyl ether with a molecular weight of 2000 was dissolved in tetrahydrofuran and added dropwise to the above reaction solution to continue the reaction for 48 hours. In the above reaction, the molar ratio of small molecule diselenodiol, 2,4-diisocyanate toluene and ...

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Abstract

The invention discloses an ROS responsive nano assembly diagnosis and treatment agent. The nano assembly diagnosis and treatment agent is formed by self-assembling a photoacoustic imaging contrast agent, an ultra-small cerium oxide nano-enzyme with a liver protection effect and ROS responsive amphiphilic molecules. The invention further discloses a preparation method and application of the ROS responsive nano assembly diagnosis and treatment agent. The nano assembly diagnosis and treatment agent provided by the invention has good biocompatibility and microenvironment responsiveness, and the ROS responsive amphiphilic molecules have good responsiveness, and can release a photoacoustic contrast agent and the ultra-small cerium oxide nano-enzyme with a liver protection effect in a liver injury oxidative stress microenvironment; and the purposes of early diagnosis and timely treatment of liver injury are achieved. The nano assembly diagnosis and treatment agent provided by the invention realizes in-situ photoacoustic imaging and nano-enzyme catalysis liver protection treatment of acute liver injury, and has good application prospects and transformation potential in the field of diagnosis and treatment of acute liver injury.

Description

technical field [0001] The invention belongs to the field of integrated critical care diagnosis and treatment, and in particular relates to a ROS-responsive nano-assembly diagnosis and treatment agent and its preparation method and application. Background technique [0002] Acute liver injury, also known as acute liver failure, is a clinical syndrome with a mortality rate of about 30%. Acute liver injury is a disease of abnormal liver function caused by alcohol, drugs, surgery, and viruses. Severe or persistent liver injury can lead to liver failure. Drug-induced liver injury is relatively common in clinical practice, and has been paid more and more attention by researchers. Due to a large population base, a wide variety of clinical drugs, widespread irregular drug use, and insufficient awareness of drug safety issues, the number of patients with acute liver injury in my country is increasing year by year, which brings a heavy burden to patients and the medical system. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/244A61K9/51A61K49/22A61P1/16B82Y5/00B82Y20/00B82Y40/00
CPCA61K33/244A61K49/225A61K9/5146B82Y5/00B82Y20/00B82Y40/00A61P1/16
Inventor 李方园吴海滨凌代舜高建青
Owner ZHEJIANG UNIV