Light-controllable metal ion delivery particle as well as preparation method and application thereof

A metal ion and particle delivery technology, applied in the field of biomedical materials, can solve problems such as poor stability of cuprous ions, achieve the effects of less harsh synthesis conditions, reduced systemic toxicity, and convenient operation

Active Publication Date: 2022-01-07
SHENZHEN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a light-controllable metal ion delivery particle and its preparation method and application, aiming to solve the problem of poor stability of cuprous ions for tumor chemokinetic therapy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Light-controllable metal ion delivery particle as well as preparation method and application thereof
  • Light-controllable metal ion delivery particle as well as preparation method and application thereof
  • Light-controllable metal ion delivery particle as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Preparation of nanoparticles of different valence copper ion delivery systems

[0052] Dissolve 1mg of ligand molecules in 1mL of dichloromethane, first add 100μL of cuprous ions (20mM), mix with ultrasonic, then add different amounts of DSPE-PEG2000 (10mg), after ultrasonic for 30s, add the above liquid to 5mL of supernatant Pure water, then sonicate for 5 minutes, remove dichloromethane by rotary evaporation, pass through a 220 μm PES membrane, transfer to a 30kD ultrafiltration tube, centrifuge at 3500 rpm, 15 minutes, 4°C. The obtained LC1 was washed twice with water and stored at 4°C in the dark for future use.

[0053] Replace the cuprous ions with the same amount of copper ions, and the subsequent operation steps remain unchanged to obtain LC2, which is stored at 4°C in the dark for future use.

[0054] Figure 2a It is the transmission electron microscope image and the corresponding hydrated particle size image of the prepared nanoparticles (LC1) co...

Embodiment 2

[0057] Example 2: Photodynamic effect evaluation of different valence copper ion delivery systems

[0058] Use a 660nm laser to irradiate the nanoparticle solution after adding DPBF, and the irradiation power is 0.2W / cm 2 , and the duration of each exposure is 10 seconds.

[0059] Figure 5 It shows the DPBF absorption changes of LC1 and LC2 at the same concentration (20 μM) under the same light power. DPBF can be used to detect singlet oxygen ( 1 o 2 ), the faster the absorption value at 415nm decreases, the more singlet oxygen is produced. Figure 5 The results show that LC1 can produce a stronger photodynamic effect than LC2.

Embodiment 3

[0060] Example 3: Evaluation of chemical kinetic effects of copper ion delivery systems in different valence states

[0061] The effects of hydroxyl radicals produced by different valence state copper ion delivery systems under different concentrations and different light powers were compared respectively. The specific conditions are as follows: add 10 μL of 30% hydrogen peroxide solution to 6 mM terephthalic acid (TA) solution, then add LC1 or LC2, each 5 μM, under different light power irradiation, monitor the concentration of TA by fluorescence spectrophotometer Changes in fluorescence intensity at 315 nm. And different concentrations of LC1 at 660nm laser 0.2W / cm 2 The change of the fluorescence intensity of TA at 315nm when the power is irradiated; the change of the fluorescence intensity of TA at 315nm under the same concentration of laser irradiation with different laser powers.

[0062] Figure 6a Indicates the comparison of the efficiencies of hydroxyl radicals pro...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention discloses a light-controllable metal ion delivery particle as well as a preparation method and application thereof. The light-controllable metal ion delivery particle comprises an organic micromolecular ligand and metal ions combined on the organic micromolecular ligand through coordination, and a chemical structural formula of the organic micromolecular ligand is shown in the specification, wherein R is one of methyl, ethyl and benzyl; and the metal ions are copper ions or cuprous ions. According to the light-controllable metal ion delivery particle, the organic micromolecular ligand can keep the valence state of the metal ions stable, and the metal ions are delivered to a tumor region through a nano self-assembly technology; besides, the organic micromolecular ligand also has the function of a photosensitizer, and the purpose of carrying out photodynamic therapy firstly and then carrying out chemodynamic therapy can be realized, so that accurate light-controlled ion release at the tumor region can be realized, and the toxicity of a whole body is reduced; and the novel drug delivery mode provides a new strategy for collaborative treatment of tumor.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a photocontrollable metal ion delivery particle and a preparation method and application thereof. Background technique [0002] Malignant tumors have become an important threat to the health of the whole people, and the number of morbidity and death cases are increasing every year. However, the treatment options for malignant tumors are limited and drug resistance is prone to occur. Therefore, it is urgent to develop new treatment modalities. In recent years, chemodynamic therapy (CDT), as an emerging tumor treatment modality, has attracted extensive attention. CDT uses metal ions such as iron and copper to catalyze hydrogen peroxide in the tumor microenvironment to generate highly toxic hydroxyl radicals (·OH) to kill tumor cells. Among many metal ions, ferrous ions (Fe 2+ ) has the highest activity, but its reaction needs to reach the maximum efficiency under th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K49/00A61K9/16A61K47/24A61K49/22A61P35/00
CPCA61K41/0033A61K41/0057A61K9/1641A61K49/0021A61K49/0054A61K49/0091A61K49/225A61P35/00Y02A50/30
Inventor 林静唐祁南黄鹏贺婷
Owner SHENZHEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap