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Desulfitobacterium ferrireducens and application thereof

A technology of sodium benzoate and strains, applied in the directions of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problem of lack of strains, and achieve the effect of improving pollution and wide application prospects.

Pending Publication Date: 2022-01-11
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Benzoic acid can be degraded anaerobically by opening the ring with nitrate, sulfate, and Fe(III) as electron acceptors. Azoarcussp.CIB, Rhodopseudomonas palustris, and Magnetospira have been isolated Microorganisms such as MS-1 (Magnetospirillum magnetotacticum MS-1) and Azoarcus evansii can metabolize benzoic acid under anaerobic conditions, but currently there are no microorganisms that can oxidize benzoic acid with Fe(III) as the only electron acceptor lack of strains

Method used

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  • Desulfitobacterium ferrireducens and application thereof
  • Desulfitobacterium ferrireducens and application thereof
  • Desulfitobacterium ferrireducens and application thereof

Examples

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preparation example Construction

[0041] Preparation of ferrihydrite: take 54gFeCl 3 ·6H 2 O was dissolved in 300mL ultrapure water, placed on a magnetic stirrer and stirred until FeCl 3 ·6H 2 O is completely dissolved, and under vigorous and uniform stirring, add 10M NaOH dropwise at a rate of 3 seconds per drop until the pH is close to 2, change to 5M NaOH dropwise until the pH is close to 5, then change to 1M NaOH and add dropwise until the pH is close to 7.0, and then stir for 30 minutes Finally, check whether the pH is stable. After the pH is stable, let the suspension stand in the dark for 2-4 hours. At this time, the pH drops slightly. Use 1M NaOH to adjust the pH to 7.0; then wash the ferrihydrite suspension with ultrapure water centrifuge at 3000g for 20min at 4°C, discard the supernatant, add ultrapure water to evenly stir the resuspended iron gel, then centrifuge again and discard the supernatant, repeat the above cleaning steps 8 times, the purpose is to remove the suspension Dissolved Cl in sol...

Embodiment 1

[0052] Embodiment 1: (Desulfitobacterium ferrireducens) PLL0 isolation, screening and identification

[0053] 1. Screening of strains

[0054] Weigh 5g of oil-contaminated soil samples collected in Qingcheng County, Qingyang City, Gansu Province, China (altitude 1260m, east longitude 107.73°, north latitude 36.14°), grind them thoroughly with a mortar, add 2g of the ground soil to 50mL of fresh rich In the culture medium (that is, benzoic acid-ferrihydrite medium), cultured in a constant temperature incubator at 30°C in the dark, and monitored the change of ferrous iron. With the redox reaction, the color of ferrihydrite gradually changed from dark red to red. Deep and finally black, this process Fe(III) is reduced to Fe(II). When the Fe(II) concentration reaches about 20mM, extract the enrichment solution with 10% (v / v) inoculum and transfer it to a fresh enrichment medium; repeat the above steps four times to fully enrich the sodium benzoate oxidation coupling ferrihydrite ...

Embodiment 2

[0074] Embodiment 2: the application of sodium benzoate degrading strain degrading sodium benzoate under anaerobic conditions

[0075] Preparation of the seed solution of the sodium benzoate degrading strain PLL0: use the ferrozine method to monitor the reduction of ferric citrate, and obtain the growth curve of PLL0 in the seed medium, and the sodium benzoate degrading strain PLL0 bacterial solution in the logarithmic phase is the seed solution . The strain PLL0 was inoculated in the seed medium at an inoculation amount of 10% (v / v), and cultured at 30° C. for 5 days under anaerobic conditions in the dark to obtain a seed solution.

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Abstract

The invention discloses desulfitobacterium ferrireducens and an application thereof. The desulfitobacterium ferrireducens is named as (desulfitobacterium ferrireducens) PLL0, and is preserved in China General Microbiological Culture Collection Center (CGMCC) located in Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing on May 13, 2021, and the preservation number is CGMCC No.22520. The desulfitobacterium ferrireducens can degrade sodium benzoate by using ferric citrate as an electron acceptor in an anaerobic environment, and has a wide application prospect in soil remediation. The desulfitobacterium ferrireducens PLL0 or a microbial agent containing the desulfitobacterium ferrireducens can be well applied to soil remediation and improvement of soil pollution caused by PAHs.

Description

technical field [0001] The invention belongs to the field of microbial system classification and environmental biotechnology, relates to a sodium benzoate degrading bacterial strain and its application, in particular to a sodium benzoate degrading bacterial strain (Desulfitobacterium ferrireducens) PLL0 and its application. Background technique [0002] Polycyclic Aromatic Hydrocarbons (PAHs) is a general term for a class of persistent organic pollutants produced by the incomplete combustion of organic matter at high temperatures. Petroleum industry production, petroleum commodity transportation process, fossil fuel combustion, coal tar consumption, and incomplete combustion of organic matter in automobile exhaust will all produce PAHs. Domestic PAHs pollution is mainly a mixed pollutant produced by fossil dye combustion and petroleum processing. In an anaerobic environment, the degradation of PAHs is more difficult due to the limitation of microbial growth. Benzoic acid is...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10Y02A50/20
Inventor 庄莉林灿芬杨贵芹
Owner JINAN UNIVERSITY
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