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Rapid response type nucleus targeting nano diagnosis and treatment probe and preparation method and application thereof

A nanoprobe and cell nucleus technology, applied in the fields of nanomaterials and nanobiomedicine, can solve problems such as side effects, and achieve the effect of high repeatability, strong operability and high practical value

Active Publication Date: 2022-01-14
XIDIAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

From the perspective of biomedical engineering, it is of great significance to study the generation, division and metastasis of tumor cells at the cellular level for the precise treatment of cancer. However, traditional treatments (such as surgical resection, chemotherapy and radiotherapy) have many side effects ; Therefore, it is of great significance to develop nanoprobes with multiple functions for diagnosis and treatment of tumor cells

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  • Rapid response type nucleus targeting nano diagnosis and treatment probe and preparation method and application thereof
  • Rapid response type nucleus targeting nano diagnosis and treatment probe and preparation method and application thereof
  • Rapid response type nucleus targeting nano diagnosis and treatment probe and preparation method and application thereof

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preparation example Construction

[0032] The embodiment of the present invention also provides a method for preparing a nucleus-targeted multifunctional nanoprobe, the preparation method comprising the following steps:

[0033] Step 1, preparation of polymer micelles loaded with target marker-hypericin complex: mix the DMSO solution of the target marker-hypericin complex with the toluene solution of stearic acid and cholesterol, heat, Purify to obtain intermediate A; add the intermediate A to the Tris-HCl solution of polymer M, stir and purify at 0-40°C to obtain the polymer micelle core;

[0034] Step 2, preparation of the middle porous Au shell layer: grow a porous Au shell layer on the outer layer of the polymer micelle core, filter and purify it with an ultrafiltration membrane, and redisperse it in PBS buffer solution to obtain a polymerized porous Au shell layer. Micellar core;

[0035] Step 3, modification of DNA-apolipoprotein conjugate: Add the Tris-HCl solution of DNA to the NaCl solution to control...

Embodiment 1

[0039] Step 1, preparation of polymer micelles loaded with targeting marker-hypericin complex: mix DMSO solution (concentration of 2.5 mg / mL) of targeting marker-hypericin complex with stearic acid ( Concentration is 1.8mg / mL) and cholesterol (concentration is 5.3mg / mL) toluene solution is mixed evenly, wherein the molar ratio of hypericin and stearic acid is 1:0.02, and the molar ratio of stearic acid and cholesterol is 1 :0.8. The mixture was heated to 40°C, reacted for 2h, then cooled, and freeze-dried at -20°C to obtain intermediate A; intermediate A was added to the Tris-HCl solution of polymer M (concentration: 2.2mmol / mL), Wherein, the mass ratio of the intermediate A to the polymer M is 1:28, stirred and reacted at 4° C. for 24 h, and purified to obtain the polymer micelle core.

[0040]Step 2, preparation of the middle porous Au shell: grow a porous Au shell on the outer layer of the polymer micelle core, and control the reaction conditions so that the pore diameter ...

Embodiment 2

[0043] Step 1, preparation of polymer micelles loaded with targeting marker-hypericin complex: mix DMSO solution (concentration of 3.65 mg / mL) of targeting marker-hypericin complex with stearic acid ( The concentration is 1.98mg / mL) and the toluene solution of cholesterol (concentration is 4.3mg / mL) is mixed evenly, wherein the molar ratio of hypericin and stearic acid is 1:0.02, and the molar ratio of stearic acid and cholesterol is 1 :0.8. The mixture was heated to 40°C, reacted for 2h, then cooled, and freeze-dried at -20°C to obtain intermediate A; intermediate A was added to the Tris-HCl solution of polymer M (concentration: 2.2mmol / mL), Wherein, the mass ratio of the intermediate A to the polymer M is 1:28, stirred and reacted at 4° C. for 24 h, and purified to obtain the polymer micelle core.

[0044] Step 2, preparation of the middle porous Au shell: grow a porous Au shell on the outer layer of the polymer micelle core, and control the reaction conditions so that the ...

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Abstract

The invention discloses a rapid response type cell nucleus targeting nano diagnosis and treatment probe and a preparation method and application thereof, and the diagnosis and treatment probe is composed of the following components: a core of a polymer micelle loaded with a targeting marker-hypericin compound; a porous Au shell layer is arranged in the middle; and the outermost layer is a conjugate for modifying a DNA-apolipoprotein . The rapid response type nucleus targeting nano diagnosis and treatment probe provided by the invention has the characteristics of rapid enrichment in cells and rapid response; the probe provided by the invention has a nuclear targeting property, and an accurate killing effect on tumor cells at a subcellular level under photostimulation is realized. The probe provided by the invention also has thermal imaging, drug controlled release and photo-thermal treatment effects, has excellent biocompatibility, and can be used for biomedical research and application; the DNA modified nano particles used in the method can rapidly penetrate through cell membranes, can be enriched in cytoplasm, have the advantages of high operability, quantity production, high repeatability and the like, and have very high practical value.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials and nanobiomedicine, and in particular relates to a fast-response nucleus-targeted nanometer diagnosis and treatment probe and its preparation method and application. Background technique [0002] Nanomaterials are a class of functional materials with unique physical and chemical properties. With the continuous progress and development of science and technology, people have developed a series of nanomaterials with great application value in the fields of medicine, optoelectronics, energy, machinery, agriculture and so on. [0003] As a killer of human health, cancer has become the primary research object in the medical field. From its clinical symptoms to its root causes, people have invested a lot of research energy. From the perspective of biomedical engineering, it is of great significance to study the generation, division and metastasis of tumor cells at the cellular level for the prec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/42A61K47/26A61P35/00A61K49/00B82Y5/00B82Y40/00B82Y30/00
CPCA61K41/0057A61K41/0052A61K47/42A61K47/26A61P35/00A61K49/0021B82Y5/00B82Y40/00B82Y30/00
Inventor 宋江鲁奇李欢张嘉嘉张喆姚博马琳于跃周慧鑫
Owner XIDIAN UNIV