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Application of trichoderma harzianum or fermentation broth thereof in treatment and/or prevention of pitaya tree rot disease

A technology for Trichoderma harzianum and fruit tree rot, which can be used in applications, fungicides, gardening tools/equipment, etc., can solve problems such as environmental pollution, unknown, and different, and achieve good control effects.

Pending Publication Date: 2022-01-28
广东省农垦南亚热带作物科技中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, fruit farmers often use chemical agents to prevent and control the rot of dragon fruit trees, such as prochloraz, acetochlor, chlorothalonil, mancozeb, carbendazim or Bordeaux mixture, etc., but the large amount and long-term use of chemical agents , not only will cause a serious threat to human health, but also often cause environmental pollution and drug resistance of pathogenic bacteria. Therefore, it has become an urgent task in the current dragon fruit production to develop an environmentally friendly biocontrol agent to efficiently prevent and treat dragon fruit tree rot. Key Issues to be Solved
[0004] Trichoderma harzianum (Trichoderma harzianum) is one of the strains with the highest cellulase activity. As an antagonistic microorganism with abundant resources in nature, it has antagonistic effects on a variety of plant pathogenic fungi, and plays an important role in the biological control of plant pathology and in promoting agricultural production. Indispensable important status, such as CN201510299615.3 discloses the preventive effect of Trichoderma harzianum on vegetable root rot, and CN202011611848.X discloses the therapeutic effect of Trichoderma harzianum on grape downy mildew, but causes different plant rots The mechanism is different, and the strains that need to be inhibited are also different, and there is no relevant research on the effect of Trichoderma harzianum on pitaya fruit tree rot. Therefore, it is still unknown whether Trichoderma harzianum has a preventive effect on the disease

Method used

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  • Application of trichoderma harzianum or fermentation broth thereof in treatment and/or prevention of pitaya tree rot disease
  • Application of trichoderma harzianum or fermentation broth thereof in treatment and/or prevention of pitaya tree rot disease

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Embodiment 1

[0028] The application of embodiment 1 Trichoderma harzianum

[0029] 1. Preparation of culture medium

[0030] Potato medium (PDA): 200g of potatoes, 20g of sucrose, 20g of agar, 1L of distilled water, natural pH. Peel the potatoes and cut them into 1cm pieces 3 The small pieces were boiled for 30 minutes, filtered with gauze, added sucrose and agar, and made up to 1L of water, mixed evenly, dispensed, sterilized at 115°C for 20 minutes, and stored at room temperature for subsequent cultivation of Trichoderma harzianum.

[0031] Two, the preparation of Trichoderma harzianum fermented liquid

[0032] S1. Strain cultivation: Inoculate the Trichoderma harzianum strain (introduced from the Hemp Research Institute of the Chinese Academy of Agricultural Sciences) on a PDA solid medium plate, and culture it statically at 25-28° C. for 7 days in the dark;

[0033] S2. Slant culture: Use an inoculation loop to dip the mycelium of Trichoderma harzianum on the plate, inoculate it on ...

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Abstract

The invention provides application of trichoderma harzianum or fermentation broth thereof in treatment and / or prevention of pitaya tree rot disease. According to the invention, the research results show that a trichoderma harzianum fermentation broth has a very good prevention and treatment effect on pitaya tree rot disease caused by the diffusion of Scytalidium dimidiatum conidium, can be used for preparing various preparations for preventing or treating pitaya fruit tree rot diseases, and provides a novel, efficient and environment-friendly biocontrol agent source for preventing and treating pitaya tree rot disease.

Description

technical field [0001] The invention belongs to the field of antifungal technology. More specifically, it relates to the application of Trichoderma harzianum or its fermented liquid in the treatment and / or prevention of pitaya fruit tree rot. Background technique [0002] The rot of dragon fruit fruit tree (also known as white silk disease, canker) is mainly caused by the infection of dragon fruit fruit tree by Scytalidium dimidiatum The disease can infect twigs and young fruits. At the beginning, it is just a small water-soaked spot, which will gradually expand and become darker in color. Especially in high temperature and high humidity weather, the disease of twigs and young fruits Spots will increase rapidly, and new branches will generally be infected, which will further cause the rot of branches and fruits, and cause serious economic losses to fruit farmers. It has become an important disease that threatens the development of my country's dragon fruit industry. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/38A01P3/00A01G7/06A01G17/00A01G13/00
CPCA01N63/38A01G7/06A01G17/00A01G13/00
Inventor 徐杨玉禹玉华张小芳杜怡安段门俊覃金转陈士伟
Owner 广东省农垦南亚热带作物科技中心
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