Application of trichoderma harzianum or fermentation broth thereof in treatment and/or prevention of pitaya tree rot disease
A technology for Trichoderma harzianum and fruit tree rot, which can be used in applications, fungicides, gardening tools/equipment, etc., can solve problems such as environmental pollution, unknown, and different, and achieve good control effects.
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Embodiment 1
[0028] The application of embodiment 1 Trichoderma harzianum
[0029] 1. Preparation of culture medium
[0030] Potato medium (PDA): 200g of potatoes, 20g of sucrose, 20g of agar, 1L of distilled water, natural pH. Peel the potatoes and cut them into 1cm pieces 3 The small pieces were boiled for 30 minutes, filtered with gauze, added sucrose and agar, and made up to 1L of water, mixed evenly, dispensed, sterilized at 115°C for 20 minutes, and stored at room temperature for subsequent cultivation of Trichoderma harzianum.
[0031] Two, the preparation of Trichoderma harzianum fermented liquid
[0032] S1. Strain cultivation: Inoculate the Trichoderma harzianum strain (introduced from the Hemp Research Institute of the Chinese Academy of Agricultural Sciences) on a PDA solid medium plate, and culture it statically at 25-28° C. for 7 days in the dark;
[0033] S2. Slant culture: Use an inoculation loop to dip the mycelium of Trichoderma harzianum on the plate, inoculate it on ...
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