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Compound microbial agent and preparation method thereof

A technology of microbial agent and microbial fermentation liquid, which is applied in the field of microorganisms, can solve the problems of unsatisfactory effect and low number of effective bacteria, and achieve the effects of enhancing plant resistance to diseases and insect pests, reducing chemical fertilizers, and reducing pesticides

Active Publication Date: 2022-01-28
MOON (GUANGZHOU) BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the different survival conditions of bacteria, the number of effective bacteria in the obtained bacterial preparation is not high, so the effect is not ideal.

Method used

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  • Compound microbial agent and preparation method thereof
  • Compound microbial agent and preparation method thereof
  • Compound microbial agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation of embodiment 1 microbial bacterial agent

[0041] (1) Take Burkholderia, Bacillus pumilus, and Bacillus subtilis, and count them after activation and fermentation. The number of effective viable bacteria in each fermentation broth is ≥ 9 billion / mL, and they are mixed according to the mass ratio of 1:1:1. Obtain microbial fermentation broth.

[0042] (2) Take 80 parts of potassium fulvic acid, 50 parts of microbial fermentation broth, 20 parts of water, and 3 parts of diammonium phosphate. After dissolving diammonium phosphate and water, put it at room temperature, add microbial fermentation broth and potassium fulvic acid Then stir well.

[0043] (3) Spray-dry the mixed liquid obtained in the second step, and divide the obtained powder to obtain the microbial agent.

Embodiment 2

[0044] Embodiment 2 Preparation of microbial inoculum

[0045] (1) Take Burkholderia and Bacillus pumilus, after activation and fermentation, count, the number of effective viable bacteria in each fermentation broth is ≥9 billion / mL, and mix them according to the mass ratio of 1:1 to prepare microbial fermentation broth.

[0046] (2) Take 80 parts of potassium fulvic acid, 50 parts of microbial fermentation broth, 20 parts of water, and 3 parts of diammonium phosphate. After dissolving diammonium phosphate and water, put it at room temperature, add microbial fermentation broth and potassium fulvic acid Then stir well.

[0047] (3) Spray-dry the mixed liquid obtained in the second step, and divide the obtained powder to obtain the microbial agent.

Embodiment 3

[0048] Embodiment 3 Preparation of microbial inoculum

[0049](1) Take Burkholderia and Bacillus pumilus, after activation and fermentation, count, the number of effective viable bacteria in each fermentation broth is ≥9 billion / mL, and mix them according to the mass ratio of 1:1 to prepare microbial fermentation broth.

[0050] (2) Take 40 parts of potassium fulvic acid, 40 parts of microbial fermentation broth, 10 parts of water, and 2 parts of diammonium phosphate, among which diammonium phosphate and water are heated and dissolved, put it at room temperature, and add microbial fermentation broth and potassium fulvic acid Then stir well.

[0051] (3) Spray-dry the mixed liquid obtained in the second step, and divide the obtained powder to obtain the microbial agent.

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Abstract

The invention provides a compound microbial agent which is characterized in that the compound microbial agent comprises at least two of Burkholderia sp.M928, Bacillus pumilus M101 and bacillus subtilis, and the Burkholderia is separated from rhizosphere soil of rubber trees in Yunnan province. The main function of the compound microbial agent is that the compound microbial agent can effectively improve the soil environment for crop growth, can be widely applied to prevention and treatment of common diseases and insect pests such as nematodes and rhizoctonia solani, improves the nutrient absorption capability of crops, enhances the disease resistance of crops, promotes growth, and improves the yield and quality.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a compound microbial bacterial agent and a preparation method thereof. Background technique [0002] The growth process of crops will be accompanied by diseases and insect pests, as well as yield and quality problems. [0003] In the 20th century, people used chemicals to solve the above problems, but gradually discovered the shortcomings of chemicals. Although they can solve the problem for a while, the resistance, pesticide residues and soil problems caused by long-term use are often difficult to reverse, and even threat to people's health. [0004] In recent years, people have turned their attention to the biological field and used natural products such as plants, animals, and bacteria to solve the above problems. source. [0005] A large number of studies and practices have shown that microbial agents can effectively improve soil, promote soil ecological restoration, and at t...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01N63/22A01P1/00A01P3/00A01P5/00A01P21/00C12R1/22C12R1/07C12R1/125
CPCC12N1/20A01N63/20A01N63/22
Inventor 曹鸿一梁锏文孙玉婷康品刘仁燕王科晶王琳
Owner MOON (GUANGZHOU) BIOTECH CO LTD
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