Photoelectric immunosensor for detecting Alzheimer's disease marker as well as preparation method and application of photoelectric immunosensor

An Alzheimer's disease, immunosensor technology, applied in the field of biochemical sensing, can solve problems such as the limitation of the number of antibodies, and achieve the effects of promoting binding, improving separation efficiency, and improving production capacity

Pending Publication Date: 2022-01-28
LONGGANG DISTRICT CENT HOSPITAL OF SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For ZnO / Au substrates, the immobilization of antibodies is mainly through Au-NH 2 bond, but the amount of immobilized antibody is limited due to the low Au content

Method used

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  • Photoelectric immunosensor for detecting Alzheimer's disease marker as well as preparation method and application of photoelectric immunosensor
  • Photoelectric immunosensor for detecting Alzheimer's disease marker as well as preparation method and application of photoelectric immunosensor
  • Photoelectric immunosensor for detecting Alzheimer's disease marker as well as preparation method and application of photoelectric immunosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Preparation of ZnO@PDA / Au electrode

[0097] 1. Growth of ZnO nanorod arrays on ITO substrates

[0098] First, a clean piece of ITO with a size of 2.5 cm × 1 cm was soaked in freshly prepared 5 mM KMnO 4 Solution, soak for 30min at room temperature. After thoroughly rinsing with deionized water, place the ITO conductive side down into the 3 ) 2 (0.1M), ammonium hydroxide (3% v / v) and ethylenediamine (1% v / v) in 10 mL of the precursor solution, and reacted in a water bath at 75° C. for 3 h. After the reaction was completed, the obtained ZnO electrode was rinsed with water, and then dried under a mild nitrogen flow for further use.

[0099] 2. Coating PDA on the ZnO electrode

[0100] The synthesized ZnO electrode was immersed in 20 mL Tris-HCl solution (10 mM, pH=8.5) containing 40 mg polydopamine, left at room temperature for 4 h, and then gently rinsed several times with deionized water. The obtained ZnO@PDA electrodes were stored below 4 °C for future use.

[0...

Embodiment 2

[0104] Characterization of ZnO@PDA / Au electrodes

[0105] The morphology, structure and composition of the ZnO@PDA / Au electrode prepared in Example 1 were tested

[0106] 1. Morphological characteristics of SEM detection electrodes

[0107] figure 2 A is the SEM image of the ZnO electrode. It can be seen from the figure that a large number of ZnO nanorods are grown on the ITO glass, with a length of about 1.2nm and a diameter of less than 200nm. The one-dimensional structure and large gaps of ZnO nanorods can provide high specific surface area, which is beneficial for light harvesting and further biomaterial assembly.

[0108] figure 2 B is the SEM image of the ZnO@PDA electrode. After being coated with PDA, the surface of the modified nanorods is rougher than that of the unmodified ZnO nanorods, indicating that PDA has successfully wrapped the ZnO nanorods to form a core / shell structure. While the original structure of ZnO nanorods remains unchanged.

[0109] figure ...

Embodiment 3

[0118] Photoelectrochemical and electrochemical properties of ZnO@PDA / Au electrode

[0119] The electrochemical behavior and photocurrent response of the ZnO@PDA / Au electrode prepared in Example 2 were tested by linear sweep voltammetry (LSV), transient photocurrent method and electrochemical impedance method (EIS).

[0120] 1. The applied voltage was between -0.05V and 0.3V, and the LSV curves of ZnO, ZnO@PDA and ZnO@PDA / Au electrodes under dark and light conditions were recorded. image 3 A shows the LSV curves of ZnO electrodes (a, d), ZnO@PDA electrodes (b, e) and ZnO@PDA / Au electrodes (c, f) under simulated sunlight irradiation. It can be seen from the figure that all the prepared photoelectrodes showed negligible currents under dark conditions. The current density of the ZnO@PDA / Au electrode is slightly higher than that of the other two electrodes, indicating that modifying ZnO with PDA and Au can improve its electrochemical performance. Under light conditions, using P...

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Abstract

The invention provides a photoelectric immunosensor for detecting an Alzheimer's disease marker as well as a preparation method and application of the photoelectric immunosensor, and constructs an ultra-sensitive photoelectric immunosensor based on a zinc oxide-polydopamine-gold nano composite material, which is used for quantitative detection of the Alzheimer's disease marker. A polydopamine film not only can be used as a sensitizer for charge separation to improve the photoelectric conversion efficiency, but also can be used as a reducing agent for in-situ deposition of Au, and also can be used for binding antibodies to improve the carrier quantity of the antibodies. Au nanoparticles are loaded on a surface of the polydopamine film, so charge transfer efficiency and the photoelectrochemical activity under visible light are further improved. The disease marker is advantaged in that the zinc oxide-polydopamine-gold nano composite material obviously enhances the light current on the photoelectrode. Under optimized conditions, the photoelectric immunosensor has a good linear relationship in a range of 1 pg/mL to 100 ng/mL, and the lower limit of detection is 0.26 pg/mL. In addition, the photoelectric immunosensor also has good selectivity, stability and reproducibility.

Description

technical field [0001] The invention relates to the field of biochemical sensing, in particular to a photoelectric immunosensor for detecting Alzheimer's disease markers, a preparation method and application thereof. Background technique [0002] Alzheimer's disease (AD) is an irreversible neurodegenerative disease mainly affecting the elderly. According to previous reports, amyloid plaques formed by the deposition of amyloid polypeptide in the brain are the main pathological features of AD. The main component of amyloid plaques in the brain is β-amyloid (Aβ). Therefore, Aβ is considered to be an important biomarker for the early diagnosis of AD. Positron emission tomography, magnetic resonance imaging, and analysis of Aβ levels in cerebrospinal fluid are routine methods for clinical diagnosis of AD. However, due to technical, cost, and invasiveness, these detection methods are difficult to popularize among the general public. Therefore, it is necessary to develop low-cos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 刘一镳何广莉徐庆李米芳张灵艳
Owner LONGGANG DISTRICT CENT HOSPITAL OF SHENZHEN
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