Preparation method of light mineral oil for embryo culture
A light mineral oil and embryo culture technology, applied in the field of embryo culture, can solve the problems of uneven quality of light mineral oil used for embryo culture, no heavy metal ion extraction, complicated device, etc. The effect of high safety and high blastocyst formation rate
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[0027] 1. Preparation of light mineral oil.
[0028] (1) Preparation of ethanol solution
[0029] Use a graduated cylinder to measure absolute ethanol and sterile water for injection at a volume ratio of 1:4, and mix them thoroughly in a glass reagent bottle.
[0030] (2) Preparation of sodium chloride solution
[0031] Accurately weigh 0.9g of sodium chloride and place it in a glass reagent bottle, use a graduated cylinder to measure 100ml of sterilized water for injection and pour it into the glass reagent bottle to fully dissolve.
[0032] (3) Configuration of ethylenediaminetetraacetic acid solution
[0033] Accurately weigh 0.01g of ethylenediaminetetraacetic acid and place it in a glass reagent bottle, use a measuring cylinder to measure 100ml of sterilized water for injection and pour it into the glass reagent bottle to fully dissolve.
[0034] (4) Preparation of light mineral oil for embryo culture
[0035] The specific operation process includes the following step...
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