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Preparation method of light mineral oil for embryo culture

A light mineral oil and embryo culture technology, applied in the field of embryo culture, can solve the problems of uneven quality of light mineral oil used for embryo culture, no heavy metal ion extraction, complicated device, etc. The effect of high safety and high blastocyst formation rate

Pending Publication Date: 2022-02-08
江苏易核科学仪器有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] At present, there are many methods for preparing light mineral oil for embryo culture, such as standard culture medium equilibrium treatment, three-distilled water washing, water washing and centrifugation, etc. The above methods are complex and time-consuming, and do not involve the extraction of heavy metal ions , resulting in uneven quality of light mineral oil for embryo culture due to the different quality of light mineral oil itself

Method used

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  • Preparation method of light mineral oil for embryo culture
  • Preparation method of light mineral oil for embryo culture
  • Preparation method of light mineral oil for embryo culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] 1. Preparation of light mineral oil.

[0028] (1) Preparation of ethanol solution

[0029] Use a graduated cylinder to measure absolute ethanol and sterile water for injection at a volume ratio of 1:4, and mix them thoroughly in a glass reagent bottle.

[0030] (2) Preparation of sodium chloride solution

[0031] Accurately weigh 0.9g of sodium chloride and place it in a glass reagent bottle, use a graduated cylinder to measure 100ml of sterilized water for injection and pour it into the glass reagent bottle to fully dissolve.

[0032] (3) Configuration of ethylenediaminetetraacetic acid solution

[0033] Accurately weigh 0.01g of ethylenediaminetetraacetic acid and place it in a glass reagent bottle, use a measuring cylinder to measure 100ml of sterilized water for injection and pour it into the glass reagent bottle to fully dissolve.

[0034] (4) Preparation of light mineral oil for embryo culture

[0035] The specific operation process includes the following step...

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Abstract

The invention discloses a preparation method of light mineral oil for embryo culture. Light mineral oil (with density of 0.84-0.86) as a raw material, ethylenediamine tetraacetic acid as a main purifying agent and sterilized water for injection as a solvent are purified by an ethanol solution, a sodium chloride solution and an ethylenediamine tetraacetic acid solution to obtain light mineral oil for embryo culture. The preparation method is simple, convenient and rapid, and the content of heavy metal, endotoxin and nucleic acid in the mineral oil can be effectively reduced. When the light mineral oil obtained through the method is used for embryo culture, a high blastocyst forming rate can be obtained. The light mineral oil is suitable for embryo culture operation of universities, colleges, scientific research institutions and reproductive centers.

Description

technical field [0001] The invention relates to the technical field of embryo culture, in particular to a method for preparing light mineral oil for embryo culture. Background technique [0002] The light mineral oil-covered droplet culture system has been widely used in the in vitro culture of mammalian early embryos. Its main purpose is to prevent the evaporation of the culture medium and maintain the stability of the pH value, osmotic pressure and humidity of the culture medium. [0003] Light mineral oil is a product of petroleum refining and processing, and has toxicity such as heavy metal ions and peroxides. Different laboratories have used many methods to verify that light mineral oils of different brands and sources are all embryotoxic when not purified, and must be tested. It can be used for embryo culture only after purification and processing. [0004] At present, there are many methods for preparing light mineral oil for embryo culture, such as standard culture ...

Claims

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Application Information

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IPC IPC(8): C10G21/02
CPCC10G21/02
Inventor 严正杰岳挺侯道荣吴媛媛刘银燕陈雪梅孙淼
Owner 江苏易核科学仪器有限公司
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