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Bacillus licheniformis extracellular polymeric substance organic selenium product as well as preparation method and application thereof

A technology of Bacillus licheniformis and extracellular polymers, which is applied in the field of preparation of organic trace elements, can solve the problems of limitations, limitations of production capacity and efficiency tolerance, and biological toxicity, and achieve low production costs and inhibition of intestinal pathogenicity. Effects of pathogenic bacteria and anti-oxidant intestinal pathogenic bacteria

Active Publication Date: 2022-02-08
ZHENGZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The organic selenium disclosed in the prior art, such as the Chinese invention patent CN107435030B, is synthesized by additionally adding sodium selenite in the Bacillus (licheniformis) fermentation medium, which has the following problems: (1) product production capacity and efficiency are easy Limited by bacterial growth ability and bacterial tolerance to sodium selenite; (2) the obtained organic selenium product is in liquid form, and sodium selenite residues are likely to exist in the organic selenium solution, which has biological toxicity; (3) licheniformis spores Bacillus can completely reduce 5mM sodium selenite, but higher concentrations can be prepared by fermentation, and organic selenium without sodium selenite residue is limited by technology
And in other similar methods in the prior art, after the organic selenium fermentation broth is obtained, the organic selenium dry powder needs to be processed through the complicated steps of bacterial cell cracking and organic solvent extraction.

Method used

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  • Bacillus licheniformis extracellular polymeric substance organic selenium product as well as preparation method and application thereof
  • Bacillus licheniformis extracellular polymeric substance organic selenium product as well as preparation method and application thereof
  • Bacillus licheniformis extracellular polymeric substance organic selenium product as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0053] Bacillus licheniformis SPW-1 preserved at -80°C was inoculated in LB agar solid medium for activation. Bacillus licheniformis was screened independently in the early stage and preserved in the China Center for Type Culture Collection, preservation number: CCTCC M 2020298, cultured at 37°C 16h, and then use seed culture to obtain seed liquid based on 37°C and 200rpm cultivation for 20h, the obtained seed liquid is inoculated into the fermentation medium according to the volume ratio of 4%, and cultivated at 37°C and 200rpm for 56h to obtain a fermented liquid, the obtained fermented liquid is sterilized, Alcohol precipitation, freeze-drying to obtain the extracellular polymer of Bacillus licheniformis.

[0054] Wherein, the LB agar solid medium is composed of raw materials of the following concentrations: tryptone 10g / L, yeast powder 5g / L, sodium chloride 10g / L, agar 14g / L.

[0055] Wherein, the seed medium is composed of raw materials with the following concentrations: ...

Embodiment 2

[0059] Weigh 0.1 g of the extracellular polymer of Bacillus licheniformis and dissolve it in water, stir and dissolve in a water bath at 60° C., and the concentration of the extracellular polymer of Bacillus licheniformis is 1.43 g / L. After the solution temperature returns to room temperature, add dropwise a sodium selenite aqueous solution of 50 mM and a 200 mM ascorbic acid aqueous solution (the molar ratio of sodium selenite to ascorbic acid is 1:4), and when the mixed solution becomes blood red, stop Sodium selenite and ascorbic acid were added dropwise, and the reaction was continued for 2h. After the reaction, centrifuge to obtain the supernatant, mix the supernatant with absolute ethanol at a volume ratio of 1:3, let it stand at 4°C for 12 hours, centrifuge to obtain a precipitate, wash with absolute ethanol for 2 to 3 times, and freeze-dry to obtain Bacillus licheniformis extracellular polymer organic selenium, wherein the content of selenium is 96.83mg / g.

Embodiment 3

[0061] Weigh 0.1 g of the extracellular polymer of Bacillus licheniformis and dissolve it in water, stir and dissolve in a water bath at 60° C., and the concentration of the extracellular polymer of Bacillus licheniformis is 1.43 g / L. After the solution temperature returns to room temperature, add dropwise a sodium selenite aqueous solution of 50 mM and a 200 mM ascorbic acid aqueous solution (the molar ratio of sodium selenite to ascorbic acid is 1:4), and when the mixed solution becomes blood red, stop Sodium selenite and ascorbic acid were added dropwise, and the reaction was continued for 2h. After the reaction, centrifuge to obtain the supernatant, mix the supernatant with absolute ethanol at a volume ratio of 1:3, let it stand at 4°C for 12 hours, centrifuge to obtain a precipitate, wash with absolute ethanol for 2 to 3 times, and freeze-dry to obtain Bacillus licheniformis extracellular polymer organic selenium, wherein the content of selenium is 103.39mg / g.

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Abstract

The invention discloses a bacillus licheniformis extracellular polymeric substance organic selenium product as well as a preparation method and application thereof. The bacillus licheniformis extracellular polymeric substance organic selenium product is prepared from a bacillus licheniformis extracellular polymeric substance and sodium selenite. In the bacillus licheniformis extracellular polymeric substance organic selenium product, the content of organic selenium is 20-150mg / g. The invention provides the bacillus licheniformis extracellular polymeric substance organic selenium which is simple in production process, mild in condition, safe and non-toxic, is in a nanosphere shape, not only can safely supplement the selenium content of animal bodies, but also has the functions of resisting oxidation and inhibiting enteropathogenic bacteria.

Description

technical field [0001] The invention relates to the technical field of preparation of organic trace elements, in particular to a Bacillus licheniformis extracellular polymer organic selenium product and its preparation method and application. Background technique [0002] As an essential trace element in organisms, selenium has important physiological significance in terms of anti-oxidation and improving immunity. However, animals cannot synthesize selenium themselves and can only achieve the purpose of supplementing selenium through external intake. Although animals can ingest a small amount of selenate, selenite and other organic selenium compounds through plants, it is far from meeting their growth and development needs. Insufficient selenium intake by animals can interfere with normal reproductive processes, and severe selenium deficiency can lead to muscular dystrophy. Therefore, adding the trace element selenium in the feed has important practical significance for the...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P9/00A23K20/20C12R1/10
CPCC12N1/20C12P9/00A23K20/30
Inventor 庄伟王志李彦卿应汉杰陈震于志丹王石磊蔡亚凡饶远陈勇刘金乐
Owner ZHENGZHOU UNIV
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