Hydrogel mask preparation method as well as product and application thereof
A hydrogel mask and composite hydrogel technology, applied in the field of cosmetics, can solve problems such as skin damage, and achieve the effects of prolonging the shelf life and promoting the growth of fibroblasts
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Embodiment 1
[0022] A hydrogel facial mask, which uses electrospinning to prepare a silk fiber film containing antibacterial nanoparticles, and then co-ferments the silk fiber and sugarcane hydrolyzate to make the bacterial fiber and silk fiber composite and cross-linked to obtain a composite hydrogel Glue mask, prepared according to the following steps:
[0023] (1) Dissolve 0.5g nano-zinc oxide ZnO NPs in a 35% silk fibroin SF solution, mix well at 60°C to obtain a concentrated spinning solution; inject the well-mixed spinning solution into a 10 mL syringe, set The electrospinning voltage is 18kv, the flow rate is 0.25 mL / h, and the electrospinning is carried out within a receiving distance of 18 cm to obtain a silk fiber membrane containing nano-zinc oxide; Autoclave for 20 minutes, dry for later use;
[0024] (2) After the bagasse is hydrolyzed and detoxified, add 1% yeast extract and 0.5% tryptone, sterilize at 121°C, 0.07~0.1MPa for 20 minutes, and use it as a culture medium; then i...
Embodiment 2
[0030] A kind of hydrogel facial mask substrate, is similar to embodiment 1 step, is prepared according to the following steps:
[0031] (1) 1g of ZnO NPs was dissolved in a 40% silk fibroin SF solution, and mixed evenly at 60°C to obtain a concentrated spinning solution; inject the mixed spinning solution into a 10 mL syringe, set the electrostatic The spinning voltage was 18 kv, the electrospinning was carried out at a flow rate of 0.25 mL / h, and within a receiving distance of 18 cm to obtain a silk fibroin fiber membrane containing nano-zinc oxide; Autoclave for 20 minutes, dry for later use;
[0032] (2) After the bagasse is hydrolyzed and detoxified, add 1% yeast extract and 0.5% tryptone, sterilize at 121°C, 0.07~0.1MPa for 20 minutes, and use it as a culture medium; then inoculate Acetobacter acetobacter with 10% inoculum In the culture medium, after mixing evenly, a fermentation broth is obtained;
[0033] (3) Pour the fermentation broth into the culture vessel equip...
Embodiment 3
[0036] A kind of hydrogel facial mask substrate, is identical with embodiment 2 steps, is prepared according to the following steps:
[0037] (1) 1g of ZnO NPs was dissolved in a 40% silk fibroin SF solution, and mixed evenly at 60°C to obtain a concentrated spinning solution; inject the mixed spinning solution into a 10 mL syringe, set the electrostatic The spinning voltage was 18 kv, the electrospinning was carried out at a flow rate of 0.25 mL / h, and within a receiving distance of 18 cm to obtain a silk fibroin fiber membrane containing nano-zinc oxide; Autoclave for 20 minutes, dry for later use;
[0038](2) After the bagasse is hydrolyzed and detoxified, add 1% yeast extract and 0.5% tryptone, sterilize at 121°C, 0.07~0.1MPa for 20 minutes, and use it as a culture medium; then inoculate Acetobacter acetobacter with 10% inoculum In the culture medium, after mixing evenly, a fermentation broth is obtained;
[0039] (3) Pour the fermentation broth into the culture vessel e...
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