Preparation method and application of histidine scorpion toxin polypeptide component

A technology for toxin polypeptide and scorpion scorpion, which is applied in the field of preparation of polypeptide components of scorpion scorpion toxin, and can solve the problem that the research on the pharmacodynamic material basis of the polypeptide and its mechanism of action is still blank and the like.

Pending Publication Date: 2022-02-11
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Mesobuthus eupeus, as a unique species in Xinjiang and even Central Asia in my country, is rich in resources and has outstanding efficacy, but the material basis of its polypeptide pharmacodynamics and its mechanism of action are still blank, which makes the toxin of Mesobuthus eupeus a traditional Chinese medicine. It is a hot spot in the study of effective substances, and it is also a breakthrough point in the development of new peptide drugs in ethnic medicine and folk medicine.

Method used

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  • Preparation method and application of histidine scorpion toxin polypeptide component
  • Preparation method and application of histidine scorpion toxin polypeptide component
  • Preparation method and application of histidine scorpion toxin polypeptide component

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] a. Stimulate the scorpion's tail segment with a high-frequency weak current stimulation method, use a small massager to collect poison, adjust the frequency of the continuous induction electric stimulation to 128 Hz, and the voltage is 4 volts, clamp one of the scorpion's tentacles with an electrode, and then use A metal clip is clamped at the 5th segment of the scorpion's back abdomen, and the other electrode is constantly in contact with the metal clip, and the venom will be discharged. If there is no response, a few drops of normal saline are dripped on the part where the electrode contacts the scorpion body to make the scorpion venom sac Glandular muscles contract to promote detoxification and obtain crude venom;

[0034] b. Wash the crude venom obtained in step a with distilled water, and then put it into a centrifuge tube containing 50 mL of ice water to collect the crude venom discharged from the tail thorn;

[0035] c. Freeze-drying the crude venom extracted in ...

Embodiment 2

[0043] a. Stimulate the scorpion's tail segment with a high-frequency weak current stimulation method, use a small massager to collect poison, adjust the frequency of the continuous induction electric stimulation to 128 Hz, and the voltage is 5 volts, clamp one of the scorpion's tentacles with an electrode, and then use A metal clip is clamped at the 5th segment of the scorpion's back abdomen, and the other electrode is constantly in contact with the metal clip, and the venom will be discharged. If there is no response, a few drops of normal saline are dripped on the part where the electrode contacts the scorpion body to make the scorpion venom sac Glandular muscles contract to promote detoxification and obtain crude venom;

[0044] b. Wash the crude venom obtained in step a with distilled water, and then put it into a centrifuge tube containing 50 mL of ice water to collect the crude venom discharged from the tail thorn;

[0045] c. Freeze-drying the crude venom extracted in ...

Embodiment 3

[0053] a. Stimulate the scorpion's tail segment with a high-frequency weak current stimulation method, use a small massager to collect poison, adjust the frequency of the continuous induction electric stimulation to 128 Hz, and the voltage is 6 volts, clamp one of the scorpion's tentacles with an electrode, and then use A metal clip is clamped at the 5th segment of the scorpion's back abdomen, and the other electrode is constantly in contact with the metal clip, and the venom will be discharged. If there is no response, a few drops of normal saline are dripped on the part where the electrode contacts the scorpion body to make the scorpion venom sac Glandular muscles contract to promote detoxification and obtain crude venom;

[0054] b. Wash the crude venom obtained in step a with distilled water, and then put it into a centrifuge tube containing 50 mL of ice water to collect the crude venom discharged from the tail thorn;

[0055] c. Freeze-dry the crude venom extracted in ste...

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Abstract

The invention discloses a preparation method and the application of a hirsute scorpion toxin anti-leucoderma polypeptide component. The method is characterized in that hirsute scorpion toxin is taken as a research object, crude toxin is extracted by an electrical stimulation method, and the hirsute scorpion toxin polypeptide component is prepared by adopting a freeze dryer, gel chromatography, ion exchange chromatography and dialysis separation. Tests show that the separation and preparation method is more scientific, the scorpion toxin polypeptide component with higher purity is obtained, the production process is simpler and more reasonable, the product quality is further guaranteed, a qualified raw material medicine is provided for further research of the scorpion toxin polypeptide. A powerful scientific basis is provided for development of medicines for treating leucoderma and sustainable utilization of scorpion venom resources.

Description

technical field [0001] The invention relates to the technical field of scorpion toxin bioactive polypeptides, in particular to a preparation method and application of a scorpion toxin polypeptide component. Background technique [0002] Vitiligo (vitiligo) is a depigmentation autoimmune skin disease of unknown etiology. It is mainly characterized by the progressive destruction and loss of functional melanocytes in the epidermis and causes white spots. The treatment cycle is long and there is no specific treatment. The incidence rate in the world is about 0.5%-2%. The main point of treatment is to regenerate the melanin in the skin lesion, so as to restore the normal skin color. At present, the pathogenesis of vitiligo has not been fully elucidated, and its occurrence is closely related to the loss of functional melanocytes. The main pathophysiological reasons include genetic susceptibility, oxidative stress-mediated melanocyte damage, melanocyte autoimmune destruction, symp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/14C07K1/16C07K1/18C07K1/34C07K1/36A61K38/17A61P17/00
CPCC07K14/43522A61P17/00A61K38/00
Inventor 阿布力米提·伊力艾合米丁·外力杨子阿吉艾克拜尔·艾萨高彦华
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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