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Application of Rgs12 as gamma-ray radiation biomarker

A gamma-ray, multi-purpose technology, applied in the application field of gamma-ray radiation biomarkers, can solve the problems of inapplicable rapid dose estimation, etc., and achieve the effect of large detection dose range, high sensitivity and short detection time

Active Publication Date: 2022-02-11
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently common radiation biological dosimeters include chromosomal aberration (“gold standard”), micronucleus analysis, comet electrophoresis, and absolute counting of lymphocytes, etc., but these methods have limitations in accurately assessing individual exposure dose and radiation response It is only applicable to the dose estimation of a small number of casualties in accidents, and not applicable to the rapid dose estimation of large-scale nuclear accident emergency medical rescue sites

Method used

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  • Application of Rgs12 as gamma-ray radiation biomarker
  • Application of Rgs12 as gamma-ray radiation biomarker
  • Application of Rgs12 as gamma-ray radiation biomarker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1. Discovery of Rgs12 Expression for Radiation Estimation

[0048]BALB / c male mice were purchased from Sibeifu (Beijing) Biotechnology Co., Ltd., grown to 8 weeks old, and given different doses (1, 2, 4 and 6Gy) of whole-body cobalt 60-γ-ray irradiation, after irradiation After 4 hours, the peripheral blood of the mice was collected, the white blood cells were separated with red blood cell lysate, and the total RNA of the samples was extracted. The RNA quality of the 5 groups of samples was tested and sequenced using an Illumina sequencer. Use DESeq software to standardize the number of counts of each sample gene, calculate the multiple of difference, and use NB (negative binomial distribution test method) to test the significance of the difference in the number of reads, and finally according to the multiple of difference and the results of the significant difference test. Screen for differential protein-coding genes. Comparing the expression changes of mRNA ...

Embodiment 2

[0056] Example 2. Verification of Rgs12 expression in human lymphoblastoid cells (AHH1)

[0057] 1. Cell culture:

[0058] AHH1 cells (Shanghai Binsui Biotechnology Co., Ltd. (C0309)) are immortalized normal human lymphoblastoid cells cultured in RPMI 1640 medium containing 10% fetal bovine serum at 37°C and 5% CO 2 Subculture in an incubator.

[0059] 2. Irradiation with cobalt 60-γ rays:

[0060] Subcultured AHH1 cells were irradiated with cobalt 60-γ rays.

[0061] Wherein, the irradiation condition of cobalt-60γ-ray irradiation is room temperature, the irradiation dose is 1Gy and 2Gy, 4Gy and 6Gy, the irradiation distance is 4 meters, and the dose rate is 66.66cGy / min. The cells of the control group (non-irradiated group) (marked as 0Gy) were harvested, and the cells 4 and 12 hours after irradiation (marked as 4h and 12h) were successively harvested.

[0062] 3. Extraction of RNA from AHH1 cells:

[0063] Cellular RNA was extracted by the Trizol method, and the specif...

Embodiment 3

[0111] Example 3. Verification of Rgs12 expression in human isolated peripheral blood

[0112] 1. Human peripheral blood collection and culture:

[0113] 6 normal young volunteers (all signed the informed consent before the experiment), aged 20-30 years, with no recent history of acute disease infection, no history of chronic diseases such as hypertension and diabetes, no history of smoking, no history of exposure to chemical poisons, For those who are not engaged in radiation-related work, 2 tubes with a total of 20ml of peripheral blood are drawn into anticoagulant tubes at a time.

[0114] 2. Cobalt 60-γ-ray irradiation:

[0115] Peripheral blood was irradiated with different doses of cobalt-60-γ rays, and the irradiated peripheral blood was transferred to a 25cm culture bottle containing an equal volume of serum medium to continue culturing. 2 Continue to grow in the incubator until harvest.

[0116] Wherein, the irradiation condition of cobalt-60-γ-ray irradiation is r...

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Abstract

The invention relates to an application of Rgs12 as a gamma-ray radiation marker, and further relates to an application of a reagent for detecting the expression quantity or relative expression quantity of Rgs12 in preparation of a product for detecting or assisting in detecting whether a subject is subjected to gamma-ray radiation or not. According to the invention, whether the to-be-detected subject suffers from gamma ray radiation can be detected, accident rescue and wounded personnel treatment can be carried out in time, and the application has important significance on gamma ray radiation treatment.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of Rgs12 as a γ-ray radiation biomarker. Background technique [0002] After nuclear radiation accidents and nuclear terrorist attacks, it is very important to conduct rapid radiation dose assessment, classified diagnosis and corresponding treatment for the wounded. Currently common radiation biological dosimeters include chromosomal aberration (“gold standard”), micronucleus analysis, comet electrophoresis, and absolute counting of lymphocytes, etc., but these methods have limitations in accurately assessing individual exposure dose and radiation response It is only applicable to the dose estimation for the treatment of a small number of accidental casualties, and not suitable for the rapid dose estimation of the emergency medical rescue scene of a large-scale nuclear accident. [0003] Therefore, there is an urgent need for a simple, fast, accurate, eas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6851
CPCC12Q1/6883C12Q1/6851C12Q2600/158C12Q2600/166C12Q2531/113C12Q2545/101C12Q2563/107Y02A50/30
Inventor 关华高山山周平坤赵红玲宋曼黎雨尘文珂
Owner ACADEMY OF MILITARY MEDICAL SCI
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