Method for enzymatic synthesis of fumaric acid trehalose ester in non-aqueous phase medium

Abstract

The invention provides a method for enzymatic synthesis of fumaric acid trehalose ester in a non-aqueous phase medium, and relates to the field of biochemical engineering. The method for enzymatic synthesis of the trehalose fumarate in the non-aqueous phase medium comprises acetone, trehalose and fumaric acid are added into the acetone, and the trehalose fumarate is catalytically synthesized by using Novozym 435 lipase, and the production process conditions are as follows: (1) the addition amount of the trehalose is 1-25 mmol / L, preferably 10 mmol / L, and (2) the addition amount of the catalyst novozym 435 lipase is 0-50 g / L, preferably 37 g / L. The method for enzymatic synthesis of the trehalose fumarate in the non-aqueous phase medium realizes enzymatic catalytic production of the trehalose fumarate for the first time, and compared with a chemical synthesis method of a structural analogue trehalose methyl fumarate, the method adopts enzymatic one-step synthesis, adopts acetone which can be used in food production as a solvent, and is single in product; the steps of protection and deprotection of a large number of groups are omitted, the steps are simple, and the method is more environmentally friendly.

Description

technical field [0001] The invention relates to the field of biochemical technology, in particular to a method for enzymatically synthesizing trehalose fumarate in a non-aqueous medium. Background technique [0002] Trehalose, known as the sugar of life, is a naturally occurring non-reducing disaccharide. It can stabilize the structure of cell membranes and proteins under harsh environmental conditions such as high temperature, high cold, high osmotic pressure, and dehydration, and effectively protect cell membranes and proteins. Molecules are invariant and inactivated, thereby maintaining the vital processes and biological characteristics of living organisms. In my country, trehalose has been approved as a common food and listed as a medical excipient. Trehalose fatty acid ester is a kind of edible surfactant with special function formed by the condensation of trehalose and fatty acid. It is widely used in food industry, washing industry, Cosmetic industry and biomedical ...

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Problems solved by technology

[0004] Invention patent ZL201010222645.1, the name of the invention: a method for enzymatically synthesizing trehalose unsaturated fatty acid diesters in an organic phase, discloses the use of lipase NOVO435 in acetone to catalyze the synthesis of trehalose oleic acid diester and linoleic acid diester ester, arachidonic acid diester and erucic acid diester, and determined the optimal process conditions, but did not involve other long-chain fatty acids other than the above-mentioned fatty acids, as well as short-chain carboxylic acids, inorganic acids, dibasic acids, etc., did not provide and trehalose fumarate, no related experiments

So far, there have been no reports on the enzymatic research and production of trehalose fumarate

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