Application of lactobacillus salivarius JYLS-372 in preparation of hangover-alleviating and liver-protecting products
A technology of JYLS-372 and Lactobacillus salivarius, which is applied in the field of probiotics, can solve the problems of cumbersome use and unclear mechanism of action, and achieve the effect of saving costs
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0039] According to the bacterial count of Lactobacillus salivarius JYLS-372 in the product is 10 billion, 15 billion, 20 billion cfu / g, Lactobacillus salivarius JYLS-372 bacterial powder and isomalto-oligosaccharide are compounded to obtain three different bacterial count specifications Anti-alcoholic and liver-protecting products.
[0040] Wherein, Lactobacillus salivarius JYLS-372 bacterium powder is made by following preparation method:
[0041] (1) Lactobacillus salivarius JYLS-372 was first activated on the MRS plate medium;
[0042] (2) Inoculate the activated Lactobacillus salivarius JYLS-372 on the MRS liquid medium at an inoculation amount of 1% (v / v) and incubate at 37°C for 24 hours to obtain the bacterial liquid;
[0043] (3) The bacteria were collected by centrifugation, washed with sterile saline and resuspended in 15wt% reconstituted skim milk to obtain a suspension;
[0044] (4) Adjust the concentration of the suspension to 1.0-2.0×10 10 cfu / mL, obtain the ...
Embodiment 2
[0046] Weigh several parts of the anti-alcoholic and liver-protecting product of Example 1 as samples, each sample weighs 1g, transfer to a preheated test tube containing 9mL simulated gastric juice, and treat at 37°C and 80r / min for 1h, 2h, 3h respectively , 4h, 3 repetitions for each treatment.
[0047]Weigh several parts of the anti-alcoholic and liver-protecting product of Example 1 as samples, each sample weighs 1g, transfer to a test tube containing 9mL simulated intestinal fluid, and treat at 37°C and 80r / min for 1h, 2h, 3h, 4h respectively, 3 replicates per treatment.
[0048] Perform gradient dilution on the treated sample solution, use the pouring method to count viable bacteria, and calculate the survival rate:
[0049] Survival rate = (number of bacteria after treatment / number of original bacteria) × 100%.
[0050] The calculation results are shown in Table 1. It can be seen that the survival rate of Lactobacillus salivarius JYLS-372 in the simulated gastrointest...
Embodiment 3
[0054] Caco-2 cells were placed in DMEM medium (purchased from Gibco) containing 10% heat-inactivated newborn calf serum and double antibodies (penicillin concentration 100 U / mL, streptomycin 1.0 μg / mL), at 37 °C, 5%CO 2 , Incubated in a carbon dioxide incubator with a relative humidity of 95%, changed the culture medium once a day, and subcultured once every 3 to 4 days. After 18 days, the cells were seeded in a 24-well culture plate at a seeding density of about 5×10 5 cfu / mL, the adhesion test was carried out after the cells grew to a monolayer.
[0055] Inoculate the activated Lactobacillus salivarius JYLS-372 in the MRS liquid medium at an inoculation amount of 1% (v / v), culture at 37°C for 12h, then centrifuge the fermentation broth at 4000g and 4°C for 10min, and collect the bacteria and washed three times with sterile PBS buffer, and resuspended the bacteria in sterile PBS buffer to obtain a concentration of 2×10 8 cfu / mL of JYLS-372 bacterial suspension.
[0056] T...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More - R&D
- Intellectual Property
- Life Sciences
- Materials
- Tech Scout
- Unparalleled Data Quality
- Higher Quality Content
- 60% Fewer Hallucinations
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com