African swine fever virus ELISA antibody detection kit

Abstract

The invention discloses an African swine fever virus ELISA antibody detection kit, and belongs to the technical field of virus detection, the kit comprises an ELISA plate coated with antigens, and the antigens are prokaryotically expressed African swine fever virus p72, p30 and p54 proteins. The ELISA plate of the kit is coated with antigens, the antigens are prokaryotically expressed African swine fever virus p72, p30 and p54 proteins, the antigens are coated with extremely low antigen content, and the kit has no cross reaction with other main swine diseases and has good specificity through a specific test; the kit is high in sensitivity, good in repeatability and long in storage life, antibodies in different periods can be effectively detected through combined use of the three kinds of protein p72, p30 and p54 of the African swine fever viruses, a basis is provided for clinical swine herd infection conditions, and the kit plays an important role in preventing and controlling African swine fever virus infection.

Description

technical field [0001] The invention belongs to the technical field of virus detection. Background technique [0002] African swine fever (ASF) is an acute, febrile and highly lethal infectious disease of pigs caused by African swine fever virus (ASFV). It is characterized by a short course of disease and a mortality rate as high as 100%. The clinical symptoms and pathological changes are similar to those of acute swine fever, with high fever, skin congestion and cyanosis, miscarriage, edema, and organ bleeding. [0003] ASF first appeared in Kenya in 1921, and became popular in Africa, Europe, South America and other countries in the 1950s and 1960s. In recent years, ASF has spread to Georgia, Armenia, Azerbaijan, Russian Federation and other neighboring countries of our country, and there is a tendency of continuous spread, which poses a serious threat to my country's pig industry. [0004] African swine fever virus is the only member of the ASF-like virus family. The di...

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Problems solved by technology

[0005] Due to the complex infection mechanism of ASFV, there is currently no effective vaccine for prevention and treatment. In the short term, early diagnosis and regionalized prevention and control management are used for prevention and control. Strict monitoring plans are adopted. Source of infection

So far, the main diagnosis of African swine fever is based on virus gene detection, through RT-PCR and partial genome sequence analysis. In the case of low virus content, it is impossible to give an accurate judgment of infection in time. In addition, due to The emergence of attenuated mutant strains and the non-continuous detoxification of individuals have brought great challenges to nucleic acid detection. Moreover, the immunogenicity of African swine fever virus is very low, and only a few proteins are immunogenic. Moreover, the immunity of different individual proteins Sensitivity is inconsistent, and detection using only one protein or two proteins is easy to cause missed detection. Therefore, it is necessary to develop fast, accurate, specific, sensitive and effective detection methods in clinical practice.