Hyaluronic acid-astaxanthin nano-composite as well as preparation method and application thereof

A nano-composite, hyaluronic acid technology, applied in application, drug combination, animal feed, etc., can solve the problem of instability of astaxanthin, achieve good biocompatibility, improve stability, and solve the effect of easy oxidation

Pending Publication Date: 2022-03-01
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above technical problems, the present invention provides a hyaluronic acid-astaxanthin nanocomposite and its preparation

Method used

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  • Hyaluronic acid-astaxanthin nano-composite as well as preparation method and application thereof
  • Hyaluronic acid-astaxanthin nano-composite as well as preparation method and application thereof
  • Hyaluronic acid-astaxanthin nano-composite as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Add 4mmol of cross-linking agent N-hydroxysuccinimide and 8mmol of hyaluronic acid into 500mL of dimethyl sulfoxide, ultrasonically dissolve at 50°C, stir at room temperature for 10min, and then add 0.5mmol of ethylenediamine under nitrogen protection, at 50°C After reacting for 12 hours, the reaction mixture was slowly poured into 30 mL of sodium hydroxide solution, and a dialysis bag with a molecular weight cut-off of 3500 Da was used for dialysis to remove impurities. The dialysis solvent and time were successively: 0.01 M NaOH dialysis for 5 hours, acetonitrile: water (1 : 1, V / V) dialyzed for 24h, distilled water dialyzed for 72h, after the end of the dialyzed, freeze-dried to obtain ethylenediamine hyaluronic acid.

[0042] Dissolve 1mmol of ethylenediamine hyaluronic acid in 10mL of sodium acetate buffer solution with a pH value of 5, then add 1mmol of astaxanthin dissolved in 2mL of ethanol, heat and stir at 50°C for 3d under a nitrogen atmosphere, and adopt a mo...

Embodiment 2

[0047] Add 2.5mmol of cross-linking agent N-hydroxysuccinimide and 5mmol of hyaluronic acid into 500mL of dimethyl sulfoxide, ultrasonically dissolve at 50°C, add 14mmol of dichloroethane, stir at room temperature for 10min, and then add 0.5mL under nitrogen atmosphere mmol ethylenediamine, react at 80°C for 2 days, use a dialysis bag with a molecular weight cut-off of 3500Da to carry out dialysis, remove the reaction solution, the dialysis solvent and time are sequentially: 0.01M NaOH dialysis for 8 hours, acetonitrile: water (1:1, V / V) Dialysis for 20 hours, dialysis with distilled water for 55 hours, and freeze-drying to obtain ethylenediamine hyaluronic acid after dialysis.

[0048] Dissolve 5mmol ethylenediamine hyaluronic acid in 10mL sodium acetate buffer solution with a pH value of 5, then add 1mmol astaxanthin dissolved in 2mL dioxane, heat and stir at 50°C for 3d under a nitrogen atmosphere, and use interception A dialysis bag with a molecular weight of 3500 Da was ...

Embodiment 3

[0051] Add 5mmol of cross-linking agent N-hydroxysuccinimide and 10mmol of hyaluronic acid into 500mL of dimethyl sulfoxide, ultrasonically dissolve at 50°C, add 14mmol of dichloroethane, stir at room temperature for 10min, and then add 0.5mmol of Ethylenediamine was reacted at 30°C for 3 days, and the dialysis bag with a molecular weight cut-off of 3500Da was used for dialysis to remove the reaction solution. The dialysis solvent and time were as follows: 0.01M NaOH dialysis for 3 hours, acetonitrile: water (1:1, V / V ) dialysis for 24 hours, distilled water dialysis for 24 hours, and freeze-drying after dialysis to obtain ethylenediamine hyaluronic acid.

[0052] Dissolve 0.5mmol ethylenediamine hyaluronic acid in 10mL sodium acetate buffer solution with a pH value of 5, then add 1mmol astaxanthin dissolved in 2mL ethanol, heat and stir at 50°C for 3d under a nitrogen atmosphere, and adopt molecular weight cut-off of A 3500Da dialysis bag was dialyzed with deionized water for...

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Abstract

The invention relates to a hyaluronic acid-astaxanthin nano-composite as well as a preparation method and application thereof, and belongs to the technical field of marine drugs. The preparation method comprises the following steps: adding hyaluronic acid into dimethyl sulfoxide, carrying out ultrasonic stirring to dissolve EDC and NHS in a protective atmosphere, stirring at room temperature for 10 min, adding ethylenediamine, carrying out a cross-linking reaction, dialyzing and freeze-drying to obtain ethylenediamine hyaluronic acid, dissolving the ethylenediamine hyaluronic acid into a buffer solution, then adding astaxanthin, and carrying out freeze-drying to obtain the astaxanthin hydrogel. And heating and stirring under a protective atmosphere, dialyzing, and freeze-drying to obtain the hyaluronic acid-astaxanthin nano-composite. According to the method, covalent bond bonding is carried out through ethidene diamine, astaxanthin is wrapped in the nano-composite through hydrophilic hyaluronic acid, the problems that astaxanthin is prone to oxidation, poor in water solubility and unstable to heat and acid are solved, and the nano-composite is free of hemolytic toxicity in vivo, good in dispersity and stability in a physiological state and simple in preparation method.

Description

technical field [0001] The invention relates to a hyaluronic acid-astaxanthin nanocomposite, a preparation method and application thereof, and belongs to the technical field of marine medicines. Background technique [0002] Astaxanthin (AST) is a ketone carotenoid that widely exists in nature, such as most crustaceans and salmonids, leaves, flowers, fruits of plants, and feathers of flamingos. It has a strong antioxidant capacity and various physiological effects, such as anti-oxidation, anti-tumor, cancer prevention, immunity enhancement, vision improvement, etc., and it is safe and effective. However, astaxanthin is a hydrophobic substance with poor water solubility, is easily oxidized by oxygen in the environment, and is sensitive to light and heat, so it is easily destroyed in a short period of time, which affects the development and utilization of astaxanthin. [0003] In the prior art, in order to solve the above problems, astaxanthin carriers are usually prepared, s...

Claims

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Application Information

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IPC IPC(8): C08B37/08A23L33/10A23L33/125A23K50/80A23K20/163A23K20/105A61P1/06A61K31/122A61K47/61A61P1/00
CPCC08B37/0072A23L33/10A23L33/125A23K50/80A23K20/163A23K20/105A61P1/06A61K31/122A61K47/61A61P1/00A23V2002/00A23V2200/30
Inventor 李红燕解万翠车红霞董秀芳宋琳杨锡洪
Owner QINGDAO UNIV OF SCI & TECH
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