Four engineering saccharomycetes capable of efficiently synthesizing intermediate products or end products in synthetic route of ginsenoside Ro and method for efficiently synthesizing intermediate products or end products in synthetic route of ginsenoside Ro

Abstract

The invention discloses four engineering saccharomycetes capable of efficiently synthesizing intermediate products or end products of a ginsenoside Ro synthesis route and a method, and belongs to the field of synthetic biology and metabolic engineering. The four engineering saccharomycetes are respectively an engineering saccharomycete CE-P1 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.23731 and capable of efficiently synthesizing calendula officinalis glycoside E, an engineering saccharomycete IVA-P1 with the preservation number of CGMCC No.23732 and capable of efficiently synthesizing panax japonicus saponin IVa, an engineering saccharomycete R1-P1 with the preservation number of CGMCC No.23733 and capable of efficiently synthesizing zingiberoside R1, and an engineering saccharomycete R1-P1 with the preservation number of CGMCC No.23732 and capable of efficiently synthesizing zingiberoside R1. The preservation number of the engineering saccharomycetes Ro-P1 is CGMCC (China General Microbiological Culture Collection Center) No.23734, and the engineering saccharomycetes Ro-P1 can be used for efficiently synthesizing ginsenoside Ro. The engineering saccharomycetes are stable, short in growth cycle, mild in culture condition, low in culture cost and high in target product yield, and efficient de novo synthesis of a target compound is realized.

Description

technical field [0001] The invention belongs to the field of synthetic biology and metabolic engineering, and specifically relates to 4 strains of engineering yeast capable of efficiently synthesizing intermediate products or final products of ginsenoside Ro synthesis pathways and a method thereof. Background technique [0002] Ginsenoside is a sterol compound, also known as triterpene saponin. It is mainly found in ginseng medicinal materials. Ginsenoside is regarded as the main active ingredient in ginseng, which has a variety of pharmacological and physiological activities, including: anti-tumor, exciting central nervous system, anti-fatigue, improving memory and learning ability, promoting DNA and RNA synthesis, relieving fatigue, delaying Aging, inhibition of platelet aggregation, anti-shock effect, improvement of myocardial ischemia and hypoxia, treatment and prevention of coronary heart disease, enhancement of myocardial function, protection of the body's own immune ...

AI Technical Summary

Problems solved by technology

And oleanane type ginsenosides exist less under natural conditions, such as rare ginsenoside Ro, which has a lower content (about 0.4%) in plants, so there are fewer studies, and ginsenoside Ro has anti-inflammatory, detoxification, and antithrombotic properties. , inhibition of acid system platelet aggregation, anti-hepatitis, and activation of macrophages have high research value, but its natural synthesis route is unknown (the last two steps of glycosyl modification enzymes have just been reported this year), and the glycosyl modification is complex (Two kinds, three in total) make it difficult to synthesize from scratch

No de novo synthesis of ginsenoside Ro and its precursor compounds bamboo ginseng saponin Ⅳa and ginger-like notoginsenoside R1 has yet been reported by microorganisms

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