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Bacillus siamensis YW17 strain, biocontrol inoculant and application of bacillus siamensis YW17 strain in prevention and control of soil-borne diseases and/or promotion of plant growth

A technology that promotes plant growth and biocontrol fungicides. It is applied in plant growth regulators, plant growth regulators, and pest control. It can solve drug residues, increase resistance of pathogenic bacteria, and affect soil micro-ecological balance and soil fertility. question

Pending Publication Date: 2022-03-04
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Soil-borne fungi exist widely, and the range of host plants is wide, which makes it difficult to control plant diseases caused by soil-borne pathogenic fungi
The traditional soil sterilization and the application of synthetic fungicides are likely to cause environmental pollution due to the toxicity and residue of drugs, and it is easy to lead to the continuous increase of pathogenic bacteria resistance, affecting soil micro-ecological balance and soil fertility

Method used

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  • Bacillus siamensis YW17 strain, biocontrol inoculant and application of bacillus siamensis YW17 strain in prevention and control of soil-borne diseases and/or promotion of plant growth
  • Bacillus siamensis YW17 strain, biocontrol inoculant and application of bacillus siamensis YW17 strain in prevention and control of soil-borne diseases and/or promotion of plant growth
  • Bacillus siamensis YW17 strain, biocontrol inoculant and application of bacillus siamensis YW17 strain in prevention and control of soil-borne diseases and/or promotion of plant growth

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preparation example Construction

[0042] The present invention also provides a preparation method of the above-mentioned biocontrol agent, comprising the following steps:

[0043] The above bacterial strains are inoculated in LB medium and cultured for 40-60 hours to obtain the biocontrol agent.

[0044] Unless otherwise specified, the present invention has no special requirements on the source of each component of the LB medium, and commercially available products well known to those skilled in the art can be used.

[0045] In the present invention, the culture temperature is preferably 28-37°C, more preferably 29-33°C, and more preferably 30°C; the culture time is preferably 45-50h, more preferably 48h.

[0046] The present invention also provides the application of the biocontrol agent or the biocontrol agent prepared by the above preparation method in the prevention and control of soil-borne diseases and / or the promotion of plant growth. In the present invention, the soil-borne diseases preferably include...

Embodiment 1

[0050] Isolation of Bacillus siamese YW17

[0051] The formulation of the separation medium is: tryptone 10g / L, NaCl 10g / L and yeast extract powder 5g / L; after adjusting the pH to 7.0, it is sterilized under high temperature and high pressure to obtain the separation medium.

[0052] The rhizosphere soil of Pinus sylvestris forest land (the pine forest of Qiuligou Town, Dunhua City (128°07′E, 43°27′N)) was divided by 10 -1 to 10 -6 Concentration gradient dilution, take 50 μL of different concentrations of soil dilution solution and heat it in an 80°C water bath for 30 minutes (to kill most of the non-bacillus bacteria), and then evenly spread it on the LB medium, repeat each dilution three times, and invert at 30°C After 48 hours, observe the growth of the strains on the plate, the shape and color of the colony. A single colony was picked and streaked on LB medium for further purification to obtain 44 different strains of Bacillus.

Embodiment 2

[0054] Bacillus and Fusarium oxysporum confrontation effect

[0055] Taking root rot pathogen Fusarium oxysporum (strain accession number: LC656545, https: / / www.ddbj.nig.ac.jp / index-e.html) as the target bacteria, 1×10 6 The spore suspension of number of spores / mL is coated on PDA plate, cultivates 7d at 28 ℃; Use 5mm hole puncher again to make bacterial block at the PDA plate edge of cultivating 7d, be placed in the center of a new PDA plate; Example 1 is separated into After activation of different bacillus on the LB plate, use a toothpick to pick the strains and connect them to the 4 corner points at 3cm from the center of the plate, and observe the size of the inhibition zone (for the antibacterial effect of some strains, see figure 1 ), the best antibacterial effect is recorded as YW17, and the antibacterial rate is 49.7% (see figure 2 ).

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Abstract

The invention relates to the technical field of microbial control, in particular to a bacillus siamensis YW17 strain, a biocontrol inoculant and application of the bacillus siamensis YW17 strain in soil-borne disease control and / or plant growth promotion. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) The YW17 strain provided by the invention can generate antibacterial protein, has an inhibition effect on fusarium oxysporum causing soil-borne diseases, has the bacteriostasis rate of 49.7%, and has the advantages of environmental protection, high efficiency and safety. Furthermore, the YW17 strain provided by the invention can secrete heteroauxin to promote plant growth.

Description

technical field [0001] The invention relates to the technical field of microbial control, in particular to a strain of bacillus siamese YW17, a biocontrol agent and its application in preventing and controlling soil-borne diseases and / or promoting plant growth. Background technique [0002] Ginseng is a traditional precious Chinese medicinal material in my country. With the shortage of wild resources, cultivated ginseng has become the mainstream of the market, and large-scale planting is currently the main body of ginseng raw material production. In order to solve the contradiction between supply and demand in ecological protection and medicinal material production, and to alleviate the pressure of ginseng land demand, ginseng cultivation has changed from logging ginseng to farmland ginseng. [0003] Generally, ginseng planting in farmland faces problems such as low nutrients and few beneficial microorganisms. How to rebuild the soil micro-ecosystem, promote the positive suc...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P21/00A01P3/00C12R1/07
CPCC12N1/20A01N63/22
Inventor 赵敏杨洪岩魏进彬
Owner NORTHEAST FORESTRY UNIVERSITY
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