Composite ACE inhibitory peptide as well as preparation method and application thereof

A technology of inhibitory peptides and dosage, which is applied in the field of compound ACE inhibitory peptides and its preparation, can solve the problems of incomplete release of biologically active peptides, improvement of bioactivity of enzymatic hydrolyzate, increased cost and complexity, etc., to achieve improved antioxidant and ACE inhibitory activity, improved yield and quality, strong antioxidant and ACE inhibitory activity effects

Active Publication Date: 2022-03-11
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Chinese invention patent CN106978460 B discloses a method for preparing biologically active peptides by enzymatically hydrolyzing the by-products of the blue scorpion. It uses a single protein material (the by-products of the processing of the blue scorpion) for enzymatic hydrolysis, which greatly limits the biological activity of the enzymatic hydrolyzate. Great increase in activity
At the same time, compared with the synergistic effect of multiple enzymes, the use of a single enzyme (trypsin) may not be able to completely release the hidden biologically active peptides in the protein sequence
Furthermore, the described method for obtaining bioactive peptides from blue trevally by-products processing includes a step of preliminary purification of the enzymatic hydrolyzate after hydrolysis, which increases the cost and complexity of the method and thus Increased product cost

Method used

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  • Composite ACE inhibitory peptide as well as preparation method and application thereof
  • Composite ACE inhibitory peptide as well as preparation method and application thereof
  • Composite ACE inhibitory peptide as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0045] A method for preparing a composite ACE inhibitory peptide, comprising the steps of:

[0046] (1) Remove the head and viscera of the blue scad, rinse well, and grind it into minced meat through a meat grinder;

[0047] (2) Mix 1 part of soybean protein isolate with 8 parts of water, process it through a colloid mill, raise the temperature to 90°C and keep it warm for 1.5 hours, then cool down to 55°C, adjust the pH of the system to 8.0, and add Novozymes with 0.7% of soybean protein isolate Alcalase 2.4L alkaline protease, 0.5% papain and 0.5% trypsin were hydrolyzed at 55°C for 5 hours, then added blue scad meat mince with 50% soybean protein isolate, and then added glutamine with 0.3% soybean protein isolate After the transaminase continued to hydrolyze for 3 hours, the temperature was raised to 95°C and kept for 15 minutes to inactivate the enzyme. Then the material is warmed to 75°C, separated through a 100-mesh vibrating sieve, and the permeate is collected into a ...

Embodiment 2

[0051] A method for preparing a composite ACE inhibitory peptide, comprising the steps of:

[0052] (1) Remove the head and viscera of the blue scad, rinse well, and grind it into minced meat through a meat grinder;

[0053] (2) Mix 1 part of soybean protein isolate with 12 parts of water, process it through a colloid mill, raise the temperature to 90°C and keep it warm for 1.0 hour, then cool down to 50°C, adjust the pH of the system to 7.5, and add Novozymes with 1.0% of soybean protein isolate Alcalase 2.4L alkaline protease, 0.2% papain and 0.6% trypsin were hydrolyzed at 50°C for 6 hours, then added blue scad meat mince with 30% soybean protein isolate, and then added glutamine with 0.2% soybean protein isolate After the transaminase continued to hydrolyze for 4 hours, the temperature was raised to 90° C. for 30 minutes to inactivate the enzyme. Then the material is warmed to 80°C, separated through a 100-mesh vibrating sieve, and the permeate is collected into a three-p...

Embodiment 3

[0057] A method for preparing a composite ACE inhibitory peptide, comprising the steps of:

[0058] (1) Remove the head and viscera of the blue scad, rinse well, and grind it into minced meat through a meat grinder;

[0059] (2) Mix 1 part of soybean protein isolate with 10 parts of water, process it through a colloid mill, raise the temperature to 90°C and keep it warm for 1.2 hours, then cool down to 60°C, adjust the pH of the system to 8.5, and add 0.5% Novozymes of soybean protein isolate Alcalase 2.4L alkaline protease, 0.4% papain and 0.3% trypsin were hydrolyzed at 60°C for 5 hours, then added blue scad meat mince with 40% soybean protein isolate, and then added glutamine with 0.15% soybean protein isolate After the transaminase continued to hydrolyze for 5 hours, the temperature was raised to 90°C and kept for 30 minutes to inactivate the enzyme. Then the material is warmed to 78°C, separated through a 100-mesh vibrating sieve, and the permeate is collected into a thr...

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Abstract

The invention discloses a composite ACE inhibitory peptide and a preparation method and application thereof.The method comprises the following steps that soybean protein isolate is mixed with water, the mixture is mixed through a colloid mill, the temperature is increased to 90-95 DEG C, heat preservation is conducted for 1.0-1.5 hours, then alkaline protease, papain and trypsin are added for hydrolysis for 4-6 hours, then blue round meat paste is added, and the mixture is stirred to be uniform; adding glutamine transaminase, continuing hydrolysis for 3-5 hours, and then performing enzyme deactivation and separation to obtain a water system layer containing the composite ACE inhibitory peptide. Animal protein (blue circle) and vegetable protein (soybean protein isolate) are used as raw materials, on the premise that protease is used for preliminarily degrading soybean protein, blue circle is added for enzymolysis, glutamine transaminase is added at the same time, and when the protease degrades the soybean protein and the blue circle protein to release functional peptide and amino acid, the amino acid content of the protein is increased. Part of peptide and amino acid are recombined to form new functional peptide, so that the oxidation resistance and ACE inhibitory activity of the enzymolysis product are further improved.

Description

technical field [0001] The invention belongs to the field of bioactive peptide medicines, and in particular relates to a composite ACE (angiotensin-converting enzyme) inhibitory peptide and its preparation method and application. Background technique [0002] The specific mechanism of hypertension is still unclear, among which oxidative stress and angiotensin-converting enzyme (ACE) are the pathogenesis of hypertension that have been studied more, which also provides a new direction for its prevention and treatment of hypertension . [0003] Oxidative stress refers to the process of oxidative damage caused by the increased production of oxygen free radicals or decreased scavenging ability in body tissues or cells, leading to the accumulation of reactive oxygen species in the body or cells. Biomedical research data show that the pathogenesis of hypertension is closely related to oxidative stress. In hypertensive patients, the body's oxidative stress and antioxidant levels ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/22A23L33/18A61K38/01A61P9/12A61P39/06
CPCC12P21/06C07K1/36C07K1/34C07K1/22A23L33/18A61K38/011A61K38/012A61P9/12A61P39/06A23V2002/00A23V2250/55A23V2200/326A23V2200/30
Inventor 徐振秋苏国万赵谋明王曙光张琪
Owner SOUTH CHINA UNIV OF TECH
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