Composite bone cement material and preparation method thereof
A kind of bone cement, liquid phase technology, applied in prosthesis, tissue regeneration, medical science and other directions, can solve the problems of surrounding tissue necrosis, shortcomings can not be ignored, lack of biological activity, etc., to improve biological activity, good operability, biological good compatibility
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Embodiment 1
[0024] 1. Preparation method of composite bone cement material
[0025] 1) Preparation of powder phase:
[0026] Biphasic calcium phosphate, its specific preparation method is: (1) two kinds of powder ingredients (the mass ratio of hydroxyapatite in biphasic calcium phosphate is 30%, the mass ratio of β-tricalcium phosphate in biphasic calcium phosphate ratio of 70%.) and agate balls according to the ball-to-material ratio of 1:2 dry mixing, ball milling for 8 hours, and passing through a 200-mesh sieve to obtain biphasic calcium phosphate powder, wherein the two powders are hydroxyl phosphorus with a molecular weight of 500 Limestone, beta-tricalcium phosphate with a molecular weight of 310.
[0027] The polymethyl methacrylate microspheres have a molecular weight of ~110,000 and a particle size of 80um.
[0028] Human hair is washed, degreased, ground, and passed through a 200-mesh sieve to obtain human hair keratin powder.
[0029] Weigh the powder phase according to the...
Embodiment 2 to 5
[0041] The sample preparation of each embodiment is with embodiment 1 process, but concrete composition is as follows table 2:
[0042] Table 2
[0043]
[0044] The prepared bone cement samples were sterilized, weighed and put into centrifuge tubes, and culture solution was added at a ratio of 0.2g:1 ml, and soaked at 37°C for 24 hours to obtain the extract. Using the MTT method: Digest the subcultured mesenchymal stem cells MSCg with trypsin, inoculate the obtained cell suspension into a 96-well plate, and add 100 μl (5×10 3 cells / ml) of the cell suspension, and the samples of each example were inoculated into 5 wells. After incubating for 6-24 hours, let the cells adhere completely, aspirate the supernatant with a pipette gun, and then add 100ul of extraction solution. Cells were cultured for 1, 3, and 5 days in the culture medium containing the extract, and then 20ul of MTT solution was added and placed in an incubator (37°C, 5% CO 2 ) incubate for 4 hours, use a pip...
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