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Styrene degrading bacterium and application thereof

A technology of styrene and degrading bacteria, applied in the field of bioengineering, can solve the problems of harmfulness to human health, air and water pollution, etc., and achieve the effect of improving the efficiency of biochemical treatment

Active Publication Date: 2022-03-22
CHAMBROAD CHEM IND RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the production, use, transportation and storage of styrene, a large amount of styrene will enter the atmosphere and water, causing air and water pollution, and harmful to human health

Method used

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  • Styrene degrading bacterium and application thereof
  • Styrene degrading bacterium and application thereof
  • Styrene degrading bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation of embodiment 1 styrene degrading bacterial agent

[0036] (1) Strain activation: take out the strains of Pseudomonas mutans stored at 4°C and place them at room temperature for 3 hours of activation. Since the strains are stored on nutrient agar medium on a test tube slant, there is no need to add nutrients during the activation process;

[0037] (2) Preparation of liquid seeds: In the aseptic operating table, use a pipette gun to take 10ml of sterile water and add it to the strain preservation tube, and use the pipette to repeatedly blow to prepare the bacterial suspension, and then inoculate the bacterial suspension into 200ml of liquid culture medium, shaking culture for 20 hours, the culture conditions are: temperature 35°C, shaking speed 170r / min;

[0038] (3) High-density fermentation: Pseudomonas mutans liquid seeds are inoculated in the corresponding fermentation medium with an inoculum size of 1.5% by volume to carry out liquid submerged fermen...

Embodiment 2

[0043] The tolerance test of embodiment 2 styrene degrading bacteria agent

[0044] Establish inorganic salt medium systems with styrene concentrations of 5,000, 10,000, 20,000, 30,000, 40,000, 50,000, 60,000, 70,000, 80,000, 90,000, 100,000, 110,000, 120,000, 130,000, 140,000, and 150,000 mg / L respectively, and set up without adding benzene Blank system of ethylene, inoculum amount of 1%, 30°C, 110r / min constant temperature shaking for 24h, OD after 24h 600 determination.

[0045] The result is as figure 1 As shown, with the increase of styrene concentration, OD 600 It showed a trend of increasing first and then decreasing, and the OD concentration was 100000mg / L 600 Reaching the maximum shows that the strain propagation speed is faster under this concentration, and the growth of the strain shows certain inhibition when the concentration exceeds 100000mg / L.

Embodiment 3

[0046] Embodiment 3 Styrene degradation effect test

[0047] The specific verification method is as follows: put the preserved Pseudomonas mutans into the sterile LB liquid medium in the ultra-clean workbench, 35°C, 180rpm shaking culture for 16-20h, the cultured bacterial liquid is 5% by volume The amount of inoculum was inserted into the LB liquid culture medium containing styrene 60000mg / L, 30 DEG C, 110r / min constant temperature vibration 24h, every 12h sampling detects the mass concentration of remaining styrene in the shaking flask, calculates the deformity of the present invention Degradation rate of styrene by Monas.

[0048] The results are shown in Table 1. The degradation rate of Pseudomonas mutans of the present invention to a solution containing 60000 mg / L styrene in 24 hours was 99.97%.

[0049] Table 1 Verification of bacterial strains on the degradation effect of styrene

[0050]

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Abstract

The invention belongs to the technical field of bioengineering, and provides a styrene degrading bacterium and application thereof. According to the present invention, the styrene degrading bacterial agent is identified as Pseudomonas plecoglossa through 16S rDNA identification, the bacterial strain has been preserved in the China General Microbiological Culture Collection Center (CGMCC) in June 2021, and the preservation number is CGMCC No.22757. The invention further provides a preparation method of the styrene degrading bacterial agent, under the optimal condition, the degradation rate of the microbial inoculum to styrene with the initial concentration of 60000mg / L for 24h is 99.97%, and the maximum tolerance concentration to styrene reaches 100000mg / L. The styrene degrading bacterial agent provided by the invention can reduce the impact of styrene on a biochemical treatment system, improves the treatment efficiency of styrene-containing wastewater, and has remarkable economic and environment-friendly benefits. Meanwhile, the strain can be applied to the field of treatment of styrene polluted wastewater and soil or styrene-containing organic waste gas.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and provides a styrene-degrading bacterium and an application thereof. Background technique [0002] Styrene is an important basic raw material in the petrochemical industry. It is widely used in the production of plastics, synthetic rubber, resins, and pharmaceuticals. It can also be used in pharmaceuticals, fuels, pesticides, and mineral processing. It is a valuable compound. The output of styrene series resins ranks third among the synthetic resins in the world, second only to PE and PVC. Polystyrene, a homopolymer of styrene, is one of the five general-purpose thermoplastic synthetic resins. It is widely used in the three fields of injection molding products, extrusion products and foam products. The demand has grown rapidly in recent years. ABS resin made by copolymerization of styrene, butadiene and acrylonitrile is the most used bulk thermoplastic engineering plastic, and it is the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/32
CPCC12N1/20C02F3/34C02F3/348C02F2101/32
Inventor 车树刚马娜娜杨传伦蔡颖辉孔凡衡傅英旬张萧萧潘冬梅刘结磊
Owner CHAMBROAD CHEM IND RES INST CO LTD
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