Method for synthesizing hexanoic acid from high-yield sludge open system

A high-yield, sludge technology, applied in the field of environmental engineering, can solve the problems of low caproic acid production, such as the tolerance of Clostridium krujiri, to strengthen the advantages of caproic acid-producing bacteria, reduce substrate competition, and increase caproic acid production Effect

Pending Publication Date: 2022-04-08
JIANGSU UNIV
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Problems solved by technology

[0004] Aiming at the problem of low hexanoic acid production in the sludge open system and the tolerance of Clostridium krujiri, the present invention provides a method for synthesizing hexanoic acid in the sludge open system, which improves the environmental concentration of the clostridium krujiri tolerance and the obtained hexanoic acid product concentration

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  • Method for synthesizing hexanoic acid from high-yield sludge open system
  • Method for synthesizing hexanoic acid from high-yield sludge open system

Examples

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Effect test

Embodiment 1

[0034] Anaerobic sludge alkali heat treatment: the anaerobic sludge taken from the river bottom is diluted with water, and the total solid content after dilution is 4.5% to 5%, and the pH of the sludge is adjusted to 12 and maintained for 6 hours. After alkali treatment, adjust its pH to 7.0, heat and boil for 30 minutes, then cool to room temperature.

[0035] Wheat straw pretreatment: the wheat straw taken from the farmland is crushed with a pulverizer to a particle size of 0.5-1.0 cm.

[0036] Cultivation of Clostridium krujiri biofilm: add 20g of wheat straw to an anaerobic bottle with a volume of 1L, take 0.5L of the culture medium and add it to the anaerobic bottle. 2 Boil for 1 min under the conditions and cool to room temperature, quickly add 8 g of absolute ethanol, and quickly seal the anaerobic bottle with a butyl stopper. After sterilization, add 1.25g NaHCO 3 sterile NaHCO 3 solution and Na containing 40 mg sodium sulfide 2 S solution. After being inoculated wi...

Embodiment 2

[0040] Anaerobic sludge alkali heat treatment: the anaerobic sludge taken from the river bottom is diluted with water, and the total solid content after dilution is 4.5% to 5%, and the pH of the sludge is adjusted to 12 and maintained for 6 hours. After alkali treatment, adjust its pH to 7.0, heat and boil for 30 minutes, then cool to room temperature.

[0041] Wheat straw pretreatment: the wheat straw taken from the farmland is crushed with a pulverizer to a particle size of 0.5-1.0 cm.

[0042] Cultivation of Clostridium krujiri biofilm: add 20g of wheat straw to an anaerobic bottle with a volume of 1L, take 0.5L of the culture medium and add it to the anaerobic bottle. 2 Boil for 1 min under the conditions and cool to room temperature, quickly add 8 g of absolute ethanol, and quickly seal the anaerobic bottle with a butyl stopper. After sterilization, add 1.25g NaHCO 3 sterile NaHCO 3 solution and Na containing 40 mg sodium sulfide 2 S solution. After being inoculated ...

Embodiment 3

[0046] Anaerobic sludge alkali heat treatment: the anaerobic sludge taken from the river bottom is diluted with water, and the total solid content after dilution is 4.5% to 5%, and the pH of the sludge is adjusted to 12 and maintained for 6 hours. After alkali treatment, adjust its pH to 7.0, heat and boil for 30 minutes, then cool to room temperature.

[0047] Wheat straw pretreatment: the wheat straw taken from the farmland is crushed with a pulverizer to a particle size of 0.5-1.0 cm.

[0048] Cultivation of Clostridium krujiri biofilm: add 20g of wheat straw to an anaerobic bottle with a volume of 1L, take 0.5L of the culture medium and add it to the anaerobic bottle. 2 Boil for 1 min under the conditions and cool to room temperature, quickly add 8 g of absolute ethanol, and quickly seal the anaerobic bottle with a butyl stopper. After sterilization, sequentially add 1.25g NaHCO 3 into sterile NaHCO 3 solution and Na containing 40 mg sodium sulfide 2 S solution. After...

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Abstract

The invention provides a high-yield method for synthesizing hexanoic acid by a sludge open system, which comprises the following steps: firstly, carrying out alkali heat treatment on anaerobic sludge, and coating clostridium kluyvei on wheat straws by taking wheat straw particles as a matrix; mixing a wheat straw carrier with a clostridium kluyvei biofilm with the anaerobic sludge subjected to alkali heat treatment according to a mass ratio of 1: 25 to construct a sludge open system; the method comprises the following steps: adding a substrate containing ethanol and potassium acetate in an oxygen-free atmosphere, supplementing a nutrient solution, sealing a fermentation container, and fermenting at 35-39 DEG C to obtain caproic acid. The clostridium kluyvei biofilm is prepared by taking the wheat straw as a carrier, so that the advantages of caproic acid producing flora are enhanced, and the caproic acid yield of a sludge open system is increased. The problem that the caproic acid yield of a sludge open system is low is solved, and a method is provided for conversion of ethanol and acetic acid wastewater into caproic acid. The method has the characteristics of high efficiency, strong strain tolerance and the like, and can be popularized in high-value conversion of ethanol and acetic acid wastewater.

Description

technical field [0001] The invention belongs to the technical field of environmental engineering and relates to a method for synthesizing hexanoic acid in an open sludge system. Background technique [0002] Hexanoic acid has a high application value in industry and can be used as a precursor to synthesize a variety of chemicals. The sludge open system does not require high-temperature sterilization during caproic acid fermentation, which can save a lot of energy. During the fermentation process, multi-layer flora with different functions can be formed on the sludge particles, and the aggregation and growth of the flora can make it more resistant to harsh environments. However, there are often hundreds of species of bacterial flora with metabolic functions in the sludge open system, and methanogenic bacteria are one of the most common flora. When used for caproic acid production, the substrate is often utilized by methanogens, resulting in lower caproic acid production. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/40C12N1/20C12N11/12C02F11/18C02F11/04C12R1/145
Inventor 张存胜刘岩孙雨暄霍书豪
Owner JIANGSU UNIV
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