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Phospholipase A2 detection kit and preparation method thereof

A detection kit, phospholipase technology, applied in measurement devices, color/spectral property measurement, instruments, etc., can solve the problem of large differences in sensitivity and stability, insufficient anti-interference ability, and inability to accurately measure the health status of patients with special constitutions and other problems, to achieve the effect of improving sensitivity and stability, improving anti-interference ability, and good emulsification and stability.

Pending Publication Date: 2022-04-08
QINGDAO HIGHTOP BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the sensitivity and stability of different batches of reagents in the existing immunoturbidimetric technology products are quite different, and the anti-interference ability is insufficient, so it is impossible to accurately measure the health status of patients with special constitutions.

Method used

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  • Phospholipase A2 detection kit and preparation method thereof
  • Phospholipase A2 detection kit and preparation method thereof
  • Phospholipase A2 detection kit and preparation method thereof

Examples

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Embodiment 1

[0039] 1. The present embodiment provides a phospholipase A2 detection kit, and the preparation method of the kit comprises the following steps:

[0040] (1) Preparation of R1 reagent:

[0041] Prepare a buffer solution according to the component content of the R1 reagent, adjust the pH to 7.8-8.0 with a pH adjusting solution, then add inorganic salts, anti-interference agents, polymerization accelerators, and lauric acid in sequence and stir evenly to obtain the R1 reagent.

[0042] In the present embodiment, buffer solution is the Tris buffer solution of 50mmol / L~100mmol / L, the hydrochloric acid solution (pH adjusting solution) of 6mol / L, and inorganic salt is sodium chloride; Anti-interference agent is Tween 20 and ethylene glycol Block polyether, polymerization accelerator is PEG6000.

[0043] After the configuration is completed, the final concentrations of the components of R1 are:

[0044]

[0045] (2) Preparation of R2 reagent:

[0046] Take latex microspheres wi...

Embodiment 2

[0052] 1. The present embodiment provides a phospholipase A2 detection kit, and the preparation method of the kit comprises the following steps:

[0053] (1) Preparation of R1 reagent:

[0054] Prepare a buffer solution according to the component content of the R1 reagent, adjust the pH to 7.8-8.0 with a pH adjusting solution, then add inorganic salts, anti-interference agents, polymerization accelerators, and lauric acid in sequence and stir evenly to obtain the R1 reagent.

[0055] In the present embodiment, buffer solution is the Tris buffer solution of 50mmol / L~100mmol / L, the hydrochloric acid solution (pH adjusting solution) of 6mol / L, and inorganic salt is sodium chloride; Anti-interference agent is Tween 20 and ethylene glycol Block polyether, polymerization accelerator is PEG6000.

[0056] After the configuration is completed, the final concentrations of the components of R1 are:

[0057]

[0058] (2) Preparation of R2 reagent:

[0059] Take latex microspheres wi...

Embodiment 3

[0065] 2. The present embodiment provides a phospholipase A2 detection kit, which includes R1 reagent and R2 reagent, and its preparation method comprises the following steps:

[0066] (1) Preparation of R1 reagent:

[0067] Dissolve the buffer solution in water according to the component content of the R1 reagent, adjust the pH to 7.8-8.0 with the pH adjusting solution, then add inorganic salts, anti-interference agents, polymerization accelerators, and lauric acid in sequence and stir evenly. R1 reagent.

[0068] In the present embodiment, buffer solution is the Tris buffer solution of 50mmol / L~100mmol / L, the hydrochloric acid solution (pH adjusting solution) of 6mol / L, and inorganic salt is sodium chloride; Anti-interference agent is Tween 20 and ethylene glycol Block polyether, polymerization accelerator is PEG6000.

[0069] After the configuration is completed, the final concentrations of the components of R1 are:

[0070]

[0071] (2) Preparation of R2 reagent:

...

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Abstract

The invention provides a phospholipase A2 detection kit and a preparation method thereof. The phospholipase A2 detection kit comprises a reagent R1 and a reagent R2, the reagent R1 comprises the following components: a buffer solution, a pH adjusting solution, inorganic salt, an anti-interference agent, a polymerization accelerator and lauric acid; the reagent R2 comprises the following components: latex particles, an activation system, a buffer solution, an Lp-PLA2 monoclonal antibody, a sealing agent, a stabilizer and a preservative; wherein the anti-interference agent comprises ethylene glycol block polyether and Tween 20. The phospholipase A2 kit provided by the invention is simple to prepare, free of steps of centrifugal resuspension and the like, and low in production cost; moreover, according to the kit, the anti-interference agent and other components are improved, so that the sensitivity and the stability of reagent detection are greatly improved, and the anti-interference capability of the kit is also improved.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to a phospholipase A2 detection kit and a preparation method thereof. Background technique [0002] Lipoprotein-associated phospholipase A2 (Lp-PLA2) belongs to the phospholipase superfamily and is a serine esterase composed of 441 amino acids with a molecular weight of 45 kDa. It is also called platelet-activating factor acetyl hydrolase (PAF-AH). Human plasma Lp-PLA2 is mainly produced by inflammation-related cells (such as macrophages, foam cells, T lymphocytes and mast cells, etc.) in atheromatous plaques and liver. Lp-PLA2 in the blood circulation exists in the form of binding to lipoprotein particles, 80% of which are combined with low-density lipoprotein (LDL) through apolipoprotein B, and the rest are combined with high-density lipoprotein (HDL), lipoprotein a (Lpa ) and very low-density lipoprotein (VLDL). Lp-PLA2 bound to LDL is transported to vulnerable areas...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N33/577
Inventor 杨帆宋金玲周佳旋史文艳刘美雪顾晓云刘万建
Owner QINGDAO HIGHTOP BIOTECH
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