Colorimetric detection method for antibiotics based on regulation and control of nano-enzyme catalytic activity by nucleic acid aptamer

Abstract

The invention discloses a colorimetric detection method for antibiotics based on regulation and control of nano-enzyme catalytic activity by nucleic acid aptamers, and the method comprises the following steps: preparing BNQDs / CeO2 nano-enzyme, functionalizing the BNQDs / CeO2 nano-enzyme by using the nucleic acid aptamers, respectively adding the functionalized BNQDs / CeO2 nano-enzyme into a plurality of sodium acetate-acetic acid buffer solution samples containing antibiotics with different concentrations, incubating, and detecting the antibiotic activity by using the nucleic acid aptamers. Adding H2O2 and a chromogenic substrate 3, 3 ', 5, 5'-tetramethyl benzidine to react, recording the absorbance value A of each sample at the wavelength of 652nm after the reaction to obtain a detection linear relation between the antibiotic concentration and the absorbance value A, and obtaining the antibiotic concentration in the to-be-detected sample according to the detection linear relation and the absorbance value of the to-be-detected sample containing the antibiotic. The colorimetric detection method has the advantages of simplicity and rapidness in operation, low cost, high sensitivity, strong specificity, strong anti-interference capability, wide detection range, low detection limit and the like.

Description

technical field [0001] The invention belongs to the technical field of biosensing and relates to a colorimetric detection method for antibiotics, in particular to a colorimetric detection method for antibiotics based on nucleic acid aptamer-regulated nanozyme catalytic activity. Background technique [0002] Antibiotics are a class of drugs with antibacterial activity, and the increase of antibiotic resistance due to abuse has attracted people's attention. Kanamycin (KAN), as a broad-spectrum antibiotic, is widely used in animal husbandry and aquaculture because of its low cost and strong antibacterial properties, but it has toxicity and serious side effects to humans. The current methods for determining KAN include: high performance liquid chromatography, liquid chromatography-mass spectrometry, or enzyme-linked immunoassay, etc. These methods have problems such as complex equipment, cumbersome operations, high detection costs, and long detection cycles. Therefore, there i...

AI Technical Summary

Problems solved by technology

However, it is still a challenge to obtain nanozymes with high catalytic activity and high substrate selectivity, which greatly limits their wide application.

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