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Preparation of carbon electrode and detection method and application of plant morin

A carbon electrode and morin technology, applied in the direction of material electrochemical variables, can solve the problems of in-situ living body detection and large plant damage, and achieve the effect of low detection limit and fast response signal

Pending Publication Date: 2022-04-12
北京市农林科学院智能装备技术研究中心
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Problems solved by technology

[0003] Conventional techniques for detecting morin are mainly ultraviolet spectrophotometry, reverse high-performance liquid chromatography, capillary electrophoresis, etc., but usually cannot achieve in-situ detection in vivo, and the damage to plants is too large, while the electrochemical method is small and convenient. Carried outdoors for real-time monitoring, the advantages of high sensitivity and strong anti-interference ability meet the detection requirements, so electrochemical technology is the most suitable

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  • Preparation of carbon electrode and detection method and application of plant morin
  • Preparation of carbon electrode and detection method and application of plant morin

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Embodiment 1

[0038] The present embodiment provides the preparation of a nickel phthalocyanine / molybdenum-tin double metal oxide-carbon electrode, such as figure 1 Shown is a schematic diagram of the preparation process of the carbon electrode in the embodiment, specifically including:

[0039] 1) MoSn(OH) 6 Preparation of nanoprecursors

[0040] Prepare the concentration of 0.2mmol / mL Co(CH 3 COO) 2 4H 2 O and 0.2 mmol / mL Na 2 SnO 3 4H 2 Aqueous solution of O. The volumes of the two solutions were 20 mL and 10 mL respectively, mixed and stirred, and 8 mL of sodium hydroxide with a concentration of 0.1 M was added dropwise to the mixed solution, and magnetically stirred at room temperature for 1 to 2 hours. Transfer the final solution to a stainless steel autoclave and place it in an oven at 150-200°C for 18-24 hours. After the autoclave is naturally cooled, the material is collected by centrifugation, washed twice with water and absolute ethanol, and then placed in a vacuum oven ...

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Abstract

The invention provides preparation of a carbon electrode, a plant morin detection method and application. The preparation of the carbon electrode comprises the following steps: 1) preparing a MoSn (OH) 6 nano precursor by taking a Co (CH3COO) 2.4 H2O aqueous solution, a Na2SnO3. 4H2O aqueous solution and NaOH as raw materials; (2) preparing a molybdenum-tin bimetallic oxide-carbon hybrid material by taking the sunflower seed husk biomass material, the MoSn (OH) 6 nano precursor and KOH as raw materials; (3) preparing a molybdenum-tin bimetallic oxide-carbon electrode by taking the molybdenum-tin bimetallic oxide-carbon hybrid material and a naphthol methanol solution as raw materials; and 4) preparing the nickel phthalocyanine / molybdenum-tin bimetallic oxide-carbon electrode by taking a CHCl3 solution of nickel phthalocyanine and the molybdenum-tin bimetallic oxide-carbon electrode as raw materials. The carbon electrode prepared by the invention can monitor morin in plants in situ in real time based on a biosensing technology, solves the problems of complex sample pretreatment and only in-vitro detection in the prior art, and utilizes a hybrid material prepared from green and environment-friendly biomass charcoal.

Description

technical field [0001] The invention relates to the technical field of living body detection, in particular to a preparation of a carbon electrode and a detection method and application of plant morin. Background technique [0002] Ozone (O 3 ) as the main component of photooxidants, causing negative effects such as inhibition of plant growth, plant dwarfing, smaller crop leaf area, premature leaf aging, and decreased photosynthetic rate, resulting in decreased crop yield and quality. Especially in industrially developed areas such as the Beijing-Tianjin-Hebei region and the Pearl River Yangtze River Delta region, the average hourly ozone concentration of more than 10% of the time is as high as 60ng·L -1 . In view of this, the study of the biological mechanism of plants under ozone stress has received widespread attention from scholars. Flavonoids are important phenolic secondary metabolites in plants. The flavonoid-morin can well scavenge superoxide anion (O 2 - ), hy...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
Inventor 秦俊青李爱学罗斌王晓冬董宏图王成侯佩臣金晓彤周亚男张晗
Owner 北京市农林科学院智能装备技术研究中心
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