Pest control method
A technology for pests and larvae, applied in the fields of pest control, botanical equipment and methods, pesticides, etc., which can solve the problems of crop quality reduction, short life cycle, and confusion of leaf anthracnose.
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Embodiment 1
[0035] The poisonous killing of embodiment 1 Spodoptera frugiperda ovum
[0036] This example provides a method for preventing and controlling the eggs of Spodoptera frugiperda, specifically using an insecticide with methyl benzoate as the active ingredient.
[0037] The test adopts the dipping method, and takes the eggs of Spodoptera frugiperda as the test object. The specific method is as follows:
[0038] Use deionized water containing 1% emulsifier (v / v) (Tween 20 and Tween 80 at a ratio of 1:1) to be configured into 5 series of MB (methyl benzoate) aqueous solutions in equal proportions, and A blank control (deionized water containing 1% emulsifier) was set. Cut the double-sided tape into 2cm lengths, stick it on one end of the glass slide, then use tweezers to peel off the paper on the tape, select green and plump egg masses of Spodoptera frugiperda within 1d age, and divide the eggs into single grains with a soft brush. Pick a single egg and stick it on the sticky t...
Embodiment 2
[0044] The poisonous killing of embodiment 2 Spodoptera frugiperda larvae
[0045] This example provides a control method for Spodoptera frugiperda larvae, specifically using an insecticide with methyl benzoate as an active ingredient.
[0046] The test adopts the feed mixing method, and takes the 3rd instar larvae of Spodoptera frugiperda as the test object, and the specific method is as follows:
[0047] Weigh 30g of artificial feed that has completely solidified (the main components are soybean powder, wheat bran powder, yeast powder, sucrose, agar, sorbic acid, vitamin C, compound vitamin solution, acetic acid, formaldehyde, etc., and the operation method refers to the artificial feed of cotton bollworm. (Liang et al., 1999)) in a ziplock bag, and then take different volumes of MB in equal proportions to prepare 6 series of MB-containing feeds. By kneading and mixing continuously, MB is evenly dispersed in the feed. Then every part of feed is divided into 3 parts and put ...
Embodiment 3
[0057] Embodiment 3 The poisoning of Spodoptera frugiperda adults
[0058] This example provides a control method for adults of Spodoptera frugiperda, specifically using an insecticide with methyl benzoate as an active ingredient.
[0059] The test adopts the food-mixing method, and the newly emerged adults of Spodoptera frugiperda are the test objects. The specific method is as follows:
[0060] Prepare 10% honey water with deionized water containing 1% emulsifier (v / v) (Tween 20 and Tween 80 at a ratio of 1:1), as a blank control, and configure 6 series in equal proportions Concentrations of MB-containing honey water (0.05%, 0.10%, 0.25%, 0.50%, 1.00% and 2.00%). Each concentration was soaked with absorbent cotton and fed to 10 newly eclovened Spodoptera frugiperda adults (no distinction between male and female) after starvation treatment for 24 hours in a 1000ml disposable lunch box, with 3 repetitions. The number of deaths at 1h, 24h, 48h and 72h after feeding was record...
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Abstract
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