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Antibacterial film and preparation method thereof

A film and lysozyme technology, applied in the field of antibacterial film and its preparation, achieves the effects of high surface area, short activity maintenance time, and reduced production cost

Pending Publication Date: 2022-04-15
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the above-mentioned shortcoming of the prior art, the object of the present invention is to provide a kind of antibacterial film and preparation method thereof, to solve the safety problem and ecological problem existing in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036] An antibacterial film disclosed by the invention, its components include: reduced graphene oxide, lysozyme and chitosan; antibacterial agent refers to the process of inhibiting the growth and reproduction of microorganisms such as bacteria and fungi or killing them within a certain period of time Microbes, a class of substances with antibacterial and bactericidal properties, can prevent objects from being damaged by the outside world, and are the key to preparing antibacterial films. Antibacterial agents applied to antibacterial films can be roughly divided into inorganic antibacterial agents, organic antibacterial agents, natural antibacterial agents, polymer antibacterial agents and photocatalytic inorganic antibacterial agents. Lysozyme, also known as N-acetyl murein hydrolase, is a glycoside hydrolase that can specifically hydrolyze bacterial cell wall peptidoglycan. It exists in biological secretions such as egg white and saliva, and can catalyze bacterial cell wall...

Embodiment 1

[0043] Example 1: food packaging

[0044] Step 1: Weigh 2 mg of reduced graphene oxide, namely RGO, and ultrasonically disperse it in 10 ml of deionized water for 30 min to obtain a RGO dispersion solution with a concentration of 0.2 mg / ml.

[0045] Step 2: Weigh 20 mg of egg white lysozyme and dissolve it completely in 20 ml of phosphate buffer solution with a pH of 5 to obtain a lysozyme solution with a concentration of 1 mg / ml.

[0046] Step 3: Add lysozyme solutions of different concentrations obtained in step 2 to the RGO dispersion solution described in step 1, stir magnetically in an ice-water bath at 4°C for 3 hours, centrifuge at 15000r / min for 10min, collect the precipitate, and use pH=6.2 , washed three times with a 0.2 mol / L phosphate buffer solution, and the product RGO-Ly was vacuum freeze-dried and stored at 4°C.

[0047] Step 4: At 25±2° C., weigh 1.3 g of chitosan with deacetylation ≥90% and dissolve it completely with 100 ml of 2% acetic acid solution, and m...

Embodiment 2

[0051] Example 2: Agricultural plastic film

[0052] Step 1: Weigh 2 mg of reduced graphene oxide, namely RGO, and ultrasonically disperse it in 10 ml of deionized water for 30 min to obtain a RGO dispersion solution with a concentration of 0.2 mg / ml.

[0053] Step 2: Weigh 15 mg of egg white lysozyme and dissolve it completely in 20 ml of phosphate buffer solution with a pH of 5 to obtain a lysozyme solution with a concentration of 0.75 mg / ml.

[0054] Step 3: Add lysozyme solutions of different concentrations obtained in step 2 to the RGO dispersion solution described in step 1, stir magnetically in an ice-water bath at 4°C for 3 hours, centrifuge at 15000r / min for 10min, collect the precipitate, and use pH=6.2 , washed three times with a 0.2 mol / L phosphate buffer solution, and the product RGO-Ly was vacuum freeze-dried and stored at 4°C.

[0055] Step 4: At 25±2° C., weigh 2.3 g of chitosan with deacetylation ≥90% and dissolve it completely with 100 ml of 2% acetic acid s...

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Abstract

The invention discloses a preparation method for preparing an antibacterial film by immobilizing lysozyme through chitosan / reduced graphene oxide, and belongs to the technical field of biological antibacterial film preparation. According to the method, lysozyme is immobilized through a reduced graphene oxide carrier, a reduced graphene oxide-lysozyme composite antibacterial agent is prepared, chitosan is used as a main film forming material, and the chitosan / reduced graphene oxide-lysozyme composite antibacterial agent is prepared. The chitosan / reduced graphene oxide immobilized lysozyme biodegradable antibacterial film is prepared from chitosan / reduced graphene oxide as a raw material, glycerol as a plasticizer and sodium citrate as a cross-linking agent by adopting a film casting method. Wherein the three main raw materials of the reduced graphene oxide, the lysozyme and the chitosan have excellent antibacterial activity and biodegradability, and the reduced graphene oxide is simultaneously used as a reinforcing agent, so that the mechanical property of the antibacterial film is improved, and the service life of the antibacterial film is prolonged.

Description

technical field [0001] The invention belongs to the technical field of biological antibacterial film preparation, and in particular relates to an antibacterial film and a preparation method thereof. Background technique [0002] Infectious diseases caused by pathogenic microorganisms have always been one of the major threats to human health. 2 Antibacterial products can effectively block bacterial cross-infection between people, people and objects, and objects and objects. Antibacterial film material is a kind of film material with both antibacterial and bactericidal properties. It is widely used in food packaging, storage bags, agricultural mulching film, textile antibacterial and other fields, which greatly reduces food pollution, excessive harmful bacteria in crops, and skin damage. The probability of disease onset. [0003] At present, antibacterial films mainly face two problems: one is safety issues, the antibacterial agents in antibacterial films will cause potentia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J5/18C08L5/08C08L89/00C08K3/04
CPCC08J5/18C08J2305/08C08J2489/00C08K3/042
Inventor 李慧庞姗姗田佳瑶王云云薛志飞龚国利
Owner SHAANXI UNIV OF SCI & TECH
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