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Composition of lactobacillus paracasei LC-37 and application of composition in reducing blood sugar

A technology of LC-37 and Lactobacillus, applied in the field of probiotics, can solve problems such as limited, and achieve the effects of relieving diabetes symptoms, reducing insulin resistance index, and regulating blood lipids

Pending Publication Date: 2022-04-22
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] It has been reported that some probiotics have a certain relieving effect on type Ⅱ diabetes, but the research and application of probiotics, especially Lactobacillus paracasei on the secretion-stimulating effect of GLP-1 are still very limited. Therefore, the development can be more effective. It is of great significance to promote the probiotics secreted by GLP-1 and improve the efficacy of probiotics in alleviating diabetes

Method used

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  • Composition of lactobacillus paracasei LC-37 and application of composition in reducing blood sugar
  • Composition of lactobacillus paracasei LC-37 and application of composition in reducing blood sugar
  • Composition of lactobacillus paracasei LC-37 and application of composition in reducing blood sugar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Lactobacillus paracasei LC-37 promotes cell secretion of GLP-1

[0055] NCI-H716 cells in 1.5×10 6 The density of cells / well was planted in a 12-well plate coated with Matrigel, cultured for 2 days, and the endocrine differentiation experiment was carried out. The medium used for endocrine differentiation was high glucose DMEM medium containing 10% fetal bovine serum and 1% double antibody. After 2 days, the DMEM medium was supplemented with KRB buffer (128.8mmol / L NaCl, 4.8mmol / L KCl, 1.2mmol / L KH 2 PO 4 , 1.2mmol / L MgSO 4 , 2.5mmol / L CaCl 2 , 5mmol / LNaHCO 3 and 10mmol / L HEPES, pH 7.4) instead, add ①concentration of 5×10 8 CFU / mL of different lactic acid bacteria strains, ②The concentration is 5×10 10 CFU / mL of different lactic acid bacteria strains, ③ The fermentation broth of the strains sampled in the stable period (the supernatant of the culture broth), three parallels for each group, placed in 37 ° C, 5% CO 2 After culturing in the incubator for ...

Embodiment 2

[0057] The development of embodiment 2 Lactobacillus paracasei-dietary fiber composition

[0058] 1. Screen the dietary fiber compounded with Lactobacillus paracasei LC-37

[0059] Adjust the final concentration of each dietary fiber in Table 1 to be 1.5%, and the initial pH is 6.5. After sterilization, draw 1% of Lactobacillus paracasei LC-37 through activation and inoculate it in the MRS medium (the dietary fiber is the carbon source) , MRS medium (glucose is the carbon source) and carbon-free medium, cultured statically at 37°C. The absorbance of the fermentation broth was measured at 660nm at 0h, 4h, 8h, 12h and 24h of culture respectively, and the growth status of the strain was tracked and investigated. And calculate the probiotic index (PI), select 3 to 5 kinds of dietary fiber that stimulate the growth of Lactobacillus paracasei LC-37 strain best, the calculation formula of PI is as follows:

[0060] PI=[(A PP24 -A PP0 )-(A PN24 -A PN0 )] / [(A PG24 -A PG0 )-(A ...

Embodiment 3

[0069] The in vitro experiment of embodiment 3 Lactobacillus paracasei-dietary fiber composition

[0070] These two compositions of Lactobacillus paracasei LC-37+1.5% galacto-oligosaccharides and Lactobacillus paracasei LC-37+1.5% isomalto-oligosaccharides screened in Example 2 are carried out in vitro experiments, specifically as follows:

[0071] 1. Effect of promoting GLP-1 secretion

[0072] NCI-H716 cells in 1.5×10 6 The density of cells / well was planted in a 12-well plate coated with Matrigel, cultured for 2 days, and the endocrine differentiation experiment was carried out. The medium used for endocrine differentiation was high glucose DMEM medium containing 10% fetal bovine serum and 1% double antibody. After 2 days, the DMEM medium was supplemented with KRB buffer (128.8mmol / L NaCl, 4.8mmol / L KCl, 1.2mmol / L KH 2 PO 4 , 1.2mmol / L MgSO 4 , 2.5mmol / L CaCl 2 , 5mmol / LNaHCO 3 and 10mmol / L HEPES, pH 7.4), add ① Lactobacillus paracasei LC-37 with dietary fiber as the ...

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Abstract

The invention relates to the technical field of probiotics, in particular to a composition of lactobacillus paracasei LC-37 and application of the composition in reducing blood sugar. According to the invention, the lactobacillus paracasei LC-37 has new functions of promoting the secretion of the glucagon-like peptide 1, reducing blood sugar and relieving the symptoms of diabetes mellitus; the lactobacillus paracasei LC-37 is further compounded with the dietary fibers, the GLP-1 secretion promoting effect of the lactobacillus paracasei LC-37 is remarkably improved, and the composition of the lactobacillus paracasei LC-37 and the dietary fibers can remarkably reduce the blood glucose of diabetic animals, relieve oral glucose tolerance damage, reduce the insulin resistance index and regulate blood fat. The traditional Chinese medicine composition can improve intestinal mucosal barrier and pancreatic tissue damage, has a certain relieving effect on type II diabetes, and is safe, reliable and free of side effects.

Description

technical field [0001] The invention relates to the technical field of probiotics, in particular to a composition of Lactobacillus paracasei LC-37 and its application in lowering blood sugar. Background technique [0002] Diabetes mellitus (TDM) is a global chronic metabolic disease. According to the World Health Organization, the incidence of TDM in both developed and developing countries is increasing year by year. More than 90% of TDM patients belong to type 2 diabetes (T2D), which is characterized by insufficient insulin secretion and insulin resistance as well as hyperglycemia, dyslipidemia and impaired glucose tolerance. The damage of pancreatic β-cells can lead to down-regulation of insulin levels in patients with T2D, so the supplementation of extra insulin can help T2D patients to control blood sugar levels. [0003] Glucagon-like peptide 1 (GLP-1) is a hormone that is mainly secreted by intestinal L cells located in the terminal ileum and colon, and the absorption...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61P3/10A61P5/50A61P1/00A23L33/135A23C9/123A23C19/00
CPCA61K35/747A61P3/10A61P5/50A61P1/00A23L33/135A23C9/1234A23C19/00A23V2002/00A23V2400/165A23V2200/328A23V2200/32
Inventor 肖然孙二娜李树森牛天娇康小红王辰元
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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