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Preparation method and application of porcine acellular dermal matrix scaffold

A technology of decellularized dermis and matrix scaffolds, applied in the field of medical wound repair, can solve the problems of extracellular matrix microenvironment that cannot simulate the growth of wound tissue, increase the burden of doctors' operations, and doubts about clinical effectiveness, and achieve a simple and efficient preparation method , Reduce immunogenic substances, strong protective effect

Inactive Publication Date: 2022-04-22
NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the use of artificial dermal scaffolds such as collagen sponges is mainly limited to serving as a dermal matrix template to guide dermal tissue regeneration. The composition is relatively single, and it cannot simulate the extracellular matrix microenvironment of wound tissue growth, and has the following disadvantages: (1) Some wounds Accompanied by hemorrhage and effusion, it often leads to the need for secondary cleaning of hemorrhage or effusion after artificial dermis surgery, which increases the operating burden of doctors; (2) For wounds with deep skin defects, artificial dermis sponge layer needs to be filled to obtain thickness and Restoration of contour; (3) When artificial dermis is used clinically, autologous skin needs to be transplanted in two stages after vascularization, and the treatment period is longer
In addition, most of these materials are expensive, technically immature, and clinical effectiveness is still in doubt
[0006] The existing acellular dermal matrix materials have the following disadvantages: the operation is more complicated, the production cycle is longer, and the efficiency is lower; it is easy to cause damage to the matrix

Method used

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  • Preparation method and application of porcine acellular dermal matrix scaffold
  • Preparation method and application of porcine acellular dermal matrix scaffold
  • Preparation method and application of porcine acellular dermal matrix scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Preparation of porcine acellular dermal matrix scaffold

[0051] In this example, the following steps were used to prepare porcine acellular dermal matrix scaffolds:

[0052] (1) Kill fresh pigs, soak them in povidone-iodine for preliminary disinfection, remove pig hairs, wash with normal saline to remove grit and impurities on the epidermis of pigskin, adjust the scale of the skin-taking drum to 1-3mm, and take the skin in reverse. Then wash repeatedly with chlorhexidine and normal saline successively, and freeze in a sealed deep-low temperature refrigerator for later use.

[0053] (2) Full-thickness skin was slowly stirred in 1mol / L sodium chloride solution containing 0.1% peracetic acid solution for 24 hours, washed repeatedly with deionized water to remove the epidermis, and then transferred to 0.5mol / L sodium hydroxide The solution was stirred slowly for 24 hours to remove all the cells to obtain a dermal matrix layer, and then the dermal matrix was ult...

Embodiment 2

[0057] Example 2: Preparation of porcine acellular dermal matrix scaffold

[0058] In this example, the following steps were used to prepare porcine acellular dermal matrix scaffolds:

[0059] (1) Obtaining porcine dermal matrix: same as in Example 1.

[0060] (2) Full-thickness skin was slowly stirred in 2mol / L sodium chloride solution containing 0.3% peracetic acid solution for 48 hours, washed repeatedly with deionized water to remove the epidermis, and then transferred to 0.5mol / L sodium hydroxide The solution was stirred slowly for 24 hours to remove all the cells to obtain a dermal matrix layer, and then the dermal matrix was ultrasonically washed repeatedly in phosphate buffer saline and deionized water solution in sequence to finally obtain a porcelain-white porcine acellular dermal matrix.

[0061] (3) Put the acellular dermal matrix into a grinder for intermittent grinding at 34000rpm / min, 30 seconds each time, cycle several times, and filter with gauze to obtain th...

Embodiment 3

[0064] Example 3: Preparation of porcine acellular dermal matrix scaffold

[0065] In this example, the following steps were used to prepare porcine acellular dermal matrix scaffolds:

[0066] (1) Obtaining porcine dermal matrix: same as in Example 1.

[0067] (2) The full-thickness skin was stirred slowly for 48 hours in a 2mol / L sodium chloride solution containing 0.5% peracetic acid solution, washed repeatedly with deionized water to remove the epidermis, and then transferred to 0.5mol / L sodium hydroxide The solution was stirred slowly for 24 hours to remove all the cells to obtain a dermal matrix layer, and then the dermal matrix was ultrasonically washed repeatedly in phosphate buffer solution and deionized water solution in sequence to finally obtain a porcelain white acellular dermal matrix.

[0068] (3) Put the acellular dermal matrix into a grinder for intermittent grinding at 34000rpm / min, 30 seconds each time, cycle several times, and filter with gauze to obtain th...

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Abstract

The invention relates to the field of medical wound material repair, in particular to a preparation method and application of a pigskin acellular dermal matrix scaffold. The method comprises the following steps: reversely taking skin from pigskin by using a skin taking drum to obtain a dermal matrix layer; respectively carrying out high salt permeation epidermal cell removal, dermal cell removal with aqueous alkali, cross-linking, homogenate grinding, acetic acid gelation, chemical cross-linking and freeze-drying shaping on the dermal matrix layer. According to the method, preparation is simple, decellularization is thorough, immunogenic substances are reduced to the maximum extent, beneficial extracellular matrix components such as collagen and elastin are mainly reserved, and the prepared composite scaffold is uniform and regular in pore, high in hydrophilicity, capable of achieving rapid vascularization and good in mechanical property, biocompatibility and tissue induction capacity. The material can be applied to repair of full-thickness defects and chronic wounds in internal surgery, and has a wide application prospect as an outer dressing and an internal implant material.

Description

technical field [0001] The invention belongs to the field of medical wound material repair, and relates to a preparation method of a matrix support, in particular to a preparation method of a porcine acellular dermal matrix support and an application of the matrix support. [0002] The method comprises: removing pig skin from a skin drum to obtain a dermal matrix layer; performing hyperosmotic salt removal of epidermal cells on the dermal matrix layer, dedermal cell removal with alkaline solution, cross-linking, homogenate grinding, and acetic acid gelation. , chemical cross-linking, freeze-drying and shaping. The method of the present invention is simple to prepare, decellularizes completely, reduces immunogenic substances to the greatest extent, mainly retains beneficial extracellular matrix components such as collagen and elastin, and the prepared composite scaffold has uniform and regular pores, strong hydrophilicity, and rapid Vascularized, with good mechanical propertie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/56A61L27/58A61L15/40A61L15/42A61L15/64
CPCA61L27/362A61L27/3687A61L27/3691A61L27/50A61L27/56A61L27/58A61L15/40A61L15/42A61L15/425A61L15/64A61L2430/40A61L2300/412
Inventor 杨磊陈良龙马军
Owner NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV