Prostate cancer cell diaphragm and preparation method thereof

Abstract

The invention relates to a prostate cancer cell diaphragm and a preparation method thereof. The prostate cancer cell diaphragm is a three-dimensional tumor diaphragm-shaped tissue formed by connecting prostate cancer cells and an extracellular matrix through a cell diaphragm technology. The prostate cancer cell diaphragm prepared by adopting the preparation method provided by the invention not only effectively retains extracellular matrix components so as to retain a cell growth microenvironment and a microstructure, but also can simulate a real prostate cancer cell growth environment; and the prostate cancer cell diaphragm can highly express prostate cancer specific protein while having operable mechanical properties, and can be used for prostate cancer animal tumor-bearing experiments and anti-prostate cancer drug screening.

Description

technical field [0001] The invention relates to the technical field of biological sciences, in particular to a prostate cancer cell membrane and a preparation method thereof. Background technique [0002] Worldwide, the incidence of prostate cancer ranks second among all malignant tumors in men. According to the data of the National Cancer Center, prostate cancer has become the tumor with the highest incidence rate in the male urinary system since 2008. In 2014, the incidence rate reached 9.8 / 100,000, ranking 6th in the incidence rate of male malignant tumors; the mortality rate It reached 4.22 / 100,000, ranking 9th among all male malignant tumors. In terms of the age of onset, the peak incidence of prostate cancer began to appear in urban areas of my country from the age of 60. Due to the extension of life expectancy, it can be predicted that the absolute incidence of prostate cancer will experience a blowout growth. The phenotypic variability of prostate cancer cells cul...

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Problems solved by technology

[0003]Using prostate cancer cell lines cultured in vitro has always been the main way to initially detect tumor genotypes, develop anti-tumor drugs, and identify prognostic markers of cancer, etc., but this There are still some limitations of this in vitro cancer research method that are difficult to overcome; tumor cells, extracellular matrix and various cytokines are the main components of the tumor cell microenvironment; usually, when the tumor cell adherent growth is close to 100%, it is necessary to use enzymes Digest to disperse to form cell suspension for research or continue passage

Under normal circumstances, adherent tumor cells grow in a single layer and only secrete a small amount of extracellular matrix, which is usually decomposed and lost during digestion, which inevitably affects the activity of cancer cells and the conduction of signaling pathways, so as to change the genes of cancer cells / Protein expression and phenotypic characteristics; ultimately resulting in differences in angiogenesis, invasion and metastasis potential between tumor cells cultured in vitro and tumors in vivo; due to these limitations, it is difficult to translate the results of tumor in vitro studies into clinical applications

[0004] However, in the prior art, in the field of prostate cancer cells, the conventional method is to use trypsin to digest the prostate cancer cell suspension, and adopt this method to obtain prostate cancer cells The process of cancer cell suspension will cause the cells to lose the secreted extracellular matrix, resulting in free single cells in the obtained prostate cancer cell suspension, which not only causes the loss of cell activity, but also cannot retain related growth factors, cell growth The microenvironment and microstructure are not conducive to the in-depth analysis and research of prostate cancer cells in the future, such as more accurate molecular genetics research of tumor cells, tumor models and drug development, etc.; in addition, cell membrane technology stimulates extracellular matrix A technology that secretes and connects a large number of cells to form a membrane-like tissue with high cell density, uniform cell distribution, and uniform texture. This technology is currently mainly used in the field of tissue engineering reconstruction and repair. Documents and patents on the preparation of prostate cancer cell membranes using cell membrane technology, and the technical means of preparing prostate cancer cell membranes using cell membrane technology is urgently needed to be developed

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