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Polypeptide with antioxidant and ACE (angiotensin converting enzyme) inhibition functions as well as preparation method and application of polypeptide

A multi-functional, anti-oxidation technology, applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., to achieve the effect of simple steps, good antioxidant activity, and high inhibitory activity

Pending Publication Date: 2022-04-22
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the proportion of dry weight, the content of protein is second only to polysaccharides. At present, the research on it is mainly focused on the biological activity of polysaccharides and mycelial proteins. There is no report on the functional polypeptides of fruiting bodies. Entity protein has broad research prospects

Method used

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  • Polypeptide with antioxidant and ACE (angiotensin converting enzyme) inhibition functions as well as preparation method and application of polypeptide
  • Polypeptide with antioxidant and ACE (angiotensin converting enzyme) inhibition functions as well as preparation method and application of polypeptide
  • Polypeptide with antioxidant and ACE (angiotensin converting enzyme) inhibition functions as well as preparation method and application of polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The extracted protein was made into a 10 mg / mL solution, and the pH value was adjusted to 9 with NaOH. Add 7% enzyme according to the mass ratio, place at 45° C., and enzymatically hydrolyze for 4 hours. Boiling bath inactivation 5min, cooling. Separation is carried out with an ultrafiltration membrane with a cut-off of 5kDa to obtain a sample below 5000Da, and then the sample is purified by liquid chromatography to obtain the functional polypeptide of Jinfu mushroom. According to high-performance liquid chromatography, its molecular weight is about 924Da, its scavenging rates for DPPH and ABTS free radicals are 35.1% and 23.1%, respectively, and its inhibition rate for ACE is 43.6%.

Embodiment 2

[0031] The extracted protein was made into a 10mg / mL solution, and the pH value was adjusted to 8.5 with NaOH. Add 5% enzyme according to the mass ratio, place at 60° C., and perform enzymatic hydrolysis for 3 hours. Boiling bath inactivation 5min, cooling. Separation is carried out with an ultrafiltration membrane with a cut-off of 5kDa to obtain a sample below 5000Da, and then the sample is purified by liquid chromatography to obtain the functional polypeptide of Jinfu mushroom. According to high-performance liquid chromatography, its molecular weight is about 1000Da, its scavenging rate for DPPH and ABTS free radicals is 19.2% and 12.1%, respectively, and its inhibition rate for ACE is 40%.

Embodiment 3

[0033] The extracted protein was made into a 10 mg / mL solution, and the pH value was adjusted to 10.5 with NaOH. Add 6% enzyme according to the mass ratio, place at 50° C., and perform enzymatic hydrolysis for 5 hours. Boiling bath inactivation 5min, cooling. Separation is carried out with an ultrafiltration membrane with a cut-off of 5kDa to obtain a sample below 5000Da, and then the sample is purified by liquid chromatography to obtain the functional polypeptide of Jinfu mushroom. According to HPLC analysis, its molecular weight is about 1200Da, and its scavenging rate for DPPH and ABTS free radicals is 26.5% and 18.4%, respectively, and its inhibition rate for ACE is 31.3%.

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Abstract

The invention discloses a polypeptide with anti-oxidation and ACE (angiotensin converting enzyme) inhibition functions as well as a preparation method and application thereof. The method comprises the following steps: grinding dried tricholoma lobayense heim sporocarp into powder by using a grinder, staying overnight, adding 80% by volume of ammonium sulfate, placing in a refrigerator at 4 DEG C for 12 hours, centrifuging in an ultra-high-speed centrifuge at 4 DEG C at a rotating speed of 15000 * g for 15 minutes to obtain a precipitate, dialyzing to remove inorganic salt ions by using a 3500 Da dialysis bag, freeze-drying to obtain a protein sample, and purifying the sample in a liquid phase to obtain the tricholoma lobayense heim protein. An in-vitro free radical scavenging experiment shows that the polypeptide has very good antioxidant activity; an ACE inhibition experiment shows that the compound has relatively high inhibition activity. At present, related reports on the activity of the tricholoma lobayense heim functional polypeptide are not found, and the tricholoma lobayense heim functional polypeptide has a very wide medical prospect.

Description

technical field [0001] The invention belongs to the technical field of intensive processing of agricultural products, and specifically relates to a polypeptide having both anti-oxidation and ACE inhibition functions, a preparation method and an application. Background technique [0002] Many human diseases are closely related to the generation of free radicals in the body. These free radicals attack the body tissues, causing oxidative damage to DNA, proteins and membrane lipids, triggering a series of chain reactions, which in turn damage the various organs of the body and cause metabolic damage. sexual injury. Therefore, it is of great significance to search for new antioxidants. [0003] Current drugs for the treatment of hypertension, such as captopril, can effectively inhibit the activity of ACE and lower blood pressure, but it has obvious side effects. Long-term use can lead to excessive blood pressure reduction, urinary system lesions, cough, taste distortion and vasc...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/30A61K38/01A61P39/06A61P9/12
CPCC12P21/06C07K1/34C07K1/303C07K1/36A61P39/06A61P9/12A61K38/00
Inventor 孟凡菊
Owner ANHUI UNIVERSITY
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