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Fungus strain for degrading polyurethane in soil and application thereof

A polyurethane and polyurethane film technology, applied in the field of microorganisms, can solve problems such as environmental pollution, and achieve the effects of solving environmental pollution problems, high feasibility and high yield

Active Publication Date: 2022-04-29
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can effectively solve the environmental pollution problem caused by the conventional treatment method of polymer material waste

Method used

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  • Fungus strain for degrading polyurethane in soil and application thereof
  • Fungus strain for degrading polyurethane in soil and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. the screening method of fungal bacterial strain of the present invention:

[0047] The fungal strains of the present invention are screened by the dilution coating plate method. Take 1 g of soil sample and shake it in 9 mL of sterile water for 1 hour, then centrifuge at 2000 rpm for 10 minutes, and serially dilute the supernatant under aseptic conditions, that is, draw 10 -1 Put 1mL of the fungal solution into a centrifuge tube filled with 9mL sterile water, shake well to obtain a concentration of 10 -2 fungal solution, in the same way, diluted 4 times successively, marked as 10 -1 、10 -2 、10 -3 、10 -4 , and finally use a pipette to draw 20 μL of the dilutions of each concentration, place them in a petri dish containing potato dextrose agar medium (PDA), smear evenly on the surface of the petri dish with a sterile glass rod, and invert the petri dish in the dark at 28°C. Cultivate in the incubator for 3-5 days, transfer the cultured fungal colony to a new PDA cu...

Embodiment 2

[0057] A method for degrading polyurethane in soil. Follow these steps:

[0058] (1) Sampling

[0059] Soil samples contaminated with explosive substances were collected in central China. Soil collection Use a sterilized auger to take the soil at a depth of 5 cm from the surface, and collect 5 samples at each test point. The collected soil samples were put into sterilized bags, mixed thoroughly, transported to the laboratory, and stored in a 4°C refrigerator.

[0060] (2) Fungal isolation

[0061] Take 1 g of soil sample and shake it in 9 mL of sterile water for 1 hour, then centrifuge at 2000 rpm for 10 minutes, and serially dilute the supernatant under aseptic conditions, that is, draw 10 -1 Put 1mL of the fungal solution into a centrifuge tube filled with 9mL sterile water, shake well to obtain a concentration of 10 -2 fungal solution, in the same way, diluted 4 times successively, marked as 10 -1 、10 -2 、10 -3 、10 -4 , and finally use a pipette to draw 20 μL of ea...

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Abstract

The invention discloses a fungus strain for degrading polyurethane in soil and application of the fungus strain, and belongs to the technical field of microorganisms. According to the method disclosed by the invention, the strain separated and purified from the soil polluted by explosive substances is collected, culture conditions are optimized, and the soil fungi capable of degrading polyurethane are screened out and can be applied to microbial degradation of waste polyurethane materials. The method disclosed by the invention is simple in process, visual in effect and reliable in result, and can also provide reference for degrading other high polymer materials. The method can be applied to microbial degradation of waste polyurethane materials.

Description

technical field [0001] The invention belongs to the technical field of microorganisms. Parts relate to a fungus for degrading polyurethane in soil and its application. Background technique [0002] Polyurethane is a synthetic polymer produced by the reaction of polyols and polyisocyanates. Routine disposal of waste polyurethane will cause environmental pollution. Most fungi in nature achieve degradation functions by decomposing lignin and indigestible soil organic matter or consuming monosaccharides, and fungi have a higher level of biodegradation enzyme activity, and various enzymes play an important role in the biodegradation of polymers. In particular, extracellular enzymes degrade polyurethane substrates by cleaving ester bonds, actively participating in the biodegradation of polymers. Therefore, people are eager to isolate the fungi with the ability to degrade polyurethane directly from the soil, so as to solve the environmental pollution problem caused by the disposa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02B09C1/10C12R1/77
CPCC12N1/14C12N1/02B09C1/105Y02W30/62
Inventor 桂恒任广聪吴世曦葛志强张天福许建初
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI