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Method for extracting L-arginine by using ion exchange method

A technology of ion exchange method and arginine, which is applied in the field of extracting L-arginine by ion exchange method, can solve the problems of unsuitability for large-scale industrial production, low yield of L-arginine, serious pollution, etc., and achieve Less impurities, high light transmittance, and strong selectivity

Pending Publication Date: 2022-05-06
XINJIANG FUFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production methods of L-arginine mainly include hydrolysis and fermentation. The hydrolysis mainly uses animal hair and blood meal as raw materials, but the cost is high, the yield is low, and the pollution is serious.
Therefore, microbial fermentation has become the most promising production technology for amino acid production. In terms of L-arginine production, microbial fermentation also shows potential advantages, but in the extraction process of microbial fermentation broth, L-arginine shows The yield is not high, the purity is low, and it is not suitable for large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method utilizing ion exchange method to extract L-arginine, it comprises the steps:

[0030] Step 1) Ceramic membrane filtration: Adjust the pH of the L-arginine fermentation broth to 4.0, add 2 times the volume of water, stir evenly, filter through ceramic membranes, remove bacteria, macromolecular proteins and other particulate impurities, and collect ceramic membranes Serum.

[0031] Step 2) Adsorption and elution with strongly acidic cation exchange resin: The ceramic membrane supernatant obtained in step 1) is adsorbed with a cation exchange resin, and eluted with 25% ammonia water to obtain an eluent of L-arginine.

[0032] Step 3) Concentration and deamination: Concentrate the L-arginine eluate obtained in step 2) in vacuum to a concentration of 1.05g / ml at a concentration temperature of 60°C, deamination, collect the L-arginine concentrate, and recover ammonia gas .

[0033] Step 4) Overflowing the weakly basic anion exchange resin: the concentrated L-argini...

Embodiment 2

[0037] A method utilizing ion exchange method to extract L-arginine, it comprises the steps:

[0038] Step 1) Ceramic membrane filtration: adjust the pH of the L-arginine fermentation broth to 4.0±0.5, add 3 times the volume of water, stir evenly, filter through ceramic membranes, remove bacteria, macromolecular proteins and other particulate impurities, collect Ceramic Membrane Clear.

[0039] Step 2) Adsorption and elution with strongly acidic cation exchange resin: The ceramic membrane supernatant obtained in step 1) is adsorbed with a cation exchange resin, and eluted with 25% ammonia water to obtain an eluent of L-arginine.

[0040] Step 3) Concentration and deamination: Concentrate the L-arginine eluate obtained in step 2) in vacuum to a concentration of 1.1g / ml, at a concentration temperature of 65°C, deamination, collect the L-arginine concentrate, and recover ammonia gas .

[0041] Step 4) Overflowing the weakly basic anion exchange resin: the concentrated L-arginin...

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Abstract

The invention belongs to the technical field of production and extraction of L-arginine, and discloses an extraction method of L-arginine, which mainly comprises the following steps: (1) ceramic membrane filtration; (2) adsorption and elution of strong acid cation exchange resin; (3) concentration and deamination; (4) overflowing the weakly alkaline anion exchange resin; (5) concentrating and crystallizing; and (6) carrying out centrifugal drying to obtain the finished product L-arginine. According to the present invention, with the anion-cation resin technology, the acidic and neutral amino acid impurities in the L-arginine can be effectively removed, such that the purity of the prepared L-arginine is high, the yield of the finished product L-arginine is improved, and the product quality is ensured.

Description

technical field [0001] The invention belongs to the technical field of production and extraction of L-arginine, and in particular relates to a method for extracting L-arginine by using an ion exchange method. Background technique [0002] L-Arginine is a basic amino acid containing guanidinium groups. The pure product is a white crystalline powder. It exists widely in various organisms and plays an important role in the growth, development and metabolism of humans and animals. It is a protein in the human body. The raw material synthesized with creatine is also an important intermediate metabolite of the urea cycle of the organism. It is a non-essential amino acid for healthy adults, but the synthesis in the body is slow; it is an essential amino acid for infants and young children, so it is a semi-essential amino acid. amino acid. [0003] L-arginine not only participates in the synthesis of hormones and physiologically active substances in the human body, but also partici...

Claims

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Application Information

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IPC IPC(8): C07C277/08C07C279/14
CPCC07C277/08C07C279/14
Inventor 张宗华冯世红崔小红王利君王奎智王飞
Owner XINJIANG FUFENG BIOTECH