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Application of BMNP in protecting intestinal tract from ionizing radiation injury

An ionizing radiation, intestinal technology, applied in the biological field, can solve problems such as deficiency and intestinal damage, achieve good protection, protect radiation damage, and maintain intestinal homeostasis and function.

Active Publication Date: 2022-05-10
中国人民解放军火箭军特色医学中心
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above-mentioned shortcoming of the prior art, the object of the present invention is to provide the application of BMNP in protecting the intestinal tract from ionizing radiation damage, which is used to solve the intestinal injury drug caused by the lack of organisms subjected to ionizing radiation in the prior art The problem

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  • Application of BMNP in protecting intestinal tract from ionizing radiation injury
  • Application of BMNP in protecting intestinal tract from ionizing radiation injury
  • Application of BMNP in protecting intestinal tract from ionizing radiation injury

Examples

Experimental program
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Effect test

Embodiment 1

[0103] Synthesis and characterization of embodiment 1 BMNP

[0104] BMNPs are readily prepared by oxidation and self-polymerization of dopamine under alkaline conditions ( Figure 1A ). The color of the reaction solution ranges from transparent to light yellow to dark brown. DLS data show that the average hydrodynamic particle size of BMNP is 210.3nm ( Figure 1B ), the polydispersity index is 0.074. The zeta potential of BMNP is -18.9mV ( Figure 1I ), which is essential to maintain good colloidal dispersion stability. Representative SEM and TEM images such as Figure 1C and Figure 1D As shown, the prepared BMNP is spherical, with good dispersion, uniform shape, compact structure, and a diameter between 107 and 183 nm. The elemental analysis of BMNP shows that the carbon atom rate accounts for 75.43%, the oxygen atom rate accounts for 18.07%, and the silicon atom rate accounts for 6.5% ( Figure 1E ). These results indicated that the preparation of BMNPs was successf...

Embodiment 2

[0105] Example 2 BMNP can improve the survival rate of mice after TBI and reduce intestinal damage

[0106] To evaluate the radioprotective effect of BMNP on lethally irradiated mice, 5 mg / kg BMNP was administered orally 24 h and 2 h before TBI ( Figure 2A ), and the mice were monitored for up to 30 days. All the mice in the IR group died within 11 days, while 2 mice in the IR+BMNP group survived to 30 days, the survival rate was significantly increased to 20%, and the average survival time after TBI increased from 6 days (IR group) to 10.2 days (IR+BMNP group )( Figure 2B ). Similarly, we treated mice with 15Gy ABI, compared with BMNP-treated group (IR+BMNP group;) ( Figure 2F ). After irradiation, the intestinal villi may be damaged and shortened to varying degrees. To determine the protective role of BMNP against radiation injury in the mouse gut, we investigated general changes in the mouse gut. 3 days after irradiation, IR reduced the contents of the intestines, ...

Embodiment 3

[0107] Example 3 BMNP protects intestinal function after TBI

[0108] Stool shape and quantity are important indicators for judging intestinal function. We observed changes in stool morphology, quantity and weight after irradiation. The feces of the IR group changed significantly ( Figure 3A ), the amount of feces at 0, 1, 3, and 7 days ( Figure 3B ) and weight ( Figure 3C )gradually decreases. Although the amount of feces also decreased in the BMNP-administered group, there was little difference in the overall amount. Serum FITC-D has been used as an indicator of intestinal paracellular permeability. Compared with IR group ( Figure 3D ), BMNP treatment significantly reduced the uptake of FITC-D in serum. In addition, DAO in serum is generally regarded as an indirect indicator of intestinal permeability, which further confirms that BMNP can maintain normal intestinal barrier function ( Figure 3D ). Immunohistochemical analysis of mouse intestinal tight junction p...

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Abstract

The invention provides an application of BMNP in protecting intestinal tracts from ionizing radiation injury. Researches prove that the nanoparticle BMNP has a very good protection effect on radiation enteritis induced by ionizing radiation, can inhibit apoptosis and pyroptosis processes of intestinal cells in a manner of scavenging free radicals, and can protect intestinal stem cells from radiation damage, thereby maintaining intestinal homeostasis and functions.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of BMNP in protecting intestinal tract from ionizing radiation damage. Background technique [0002] When cancer patients receive radiation therapy to the abdomen, pelvis, or rectum, the bowel may be exposed to radiation damage. Its main symptoms include anorexia, vomiting, dehydration, pain, abdominal distension, diarrhea, nausea, systemic infection, and rectal bleeding. In extreme cases, septic shock and death may also occur, that is, radiation enteropathy. Hemorrhagic, pro-fibrotic pathological process. Radiation enteropathy is divided into early or delayed radiation enteropathy according to the time of onset after radiation therapy. Delayed radiation enteropathy, mainly manifested by vascular sclerosis and progressive intestinal wall fibrosis, is one of the main problems of cancer survivors; more fatal is early radiation enteropathy (intestinal disease) that occ...

Claims

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Application Information

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IPC IPC(8): A61K31/404A61P39/00A61P39/06A61P1/00A61K31/137B82Y5/00A61K31/439
CPCA61K31/404A61K31/137A61P39/00A61P39/06A61P1/00B82Y5/00A61K31/439Y02A50/30
Inventor 东肃河李峰生刘恒贾舒寒江其生于慧杰陈忠民王思念李伟彭仁军
Owner 中国人民解放军火箭军特色医学中心
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