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Bacillus subtilis for degrading vomitoxin and application thereof

A technology of Bacillus subtilis and DON, applied in the field of microorganisms, can solve the problems of loss of nutrients, low specificity, secondary pollution of feed, etc., and achieve the effect of reducing harm

Pending Publication Date: 2022-05-20
广西优比特生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The physical method is mainly to add an adsorbent to adsorb vomitoxin to achieve the effect of detoxification, but this method is not specific and is easy to absorb other nutrients, which will cause the loss of nutrients in the feed and affect the nutritional quality and palatability of the feed Chemical method is easy to leave some toxic substances, which will cause secondary pollution to the feed and seriously affect the utilization rate of the feed

Method used

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  • Bacillus subtilis for degrading vomitoxin and application thereof
  • Bacillus subtilis for degrading vomitoxin and application thereof
  • Bacillus subtilis for degrading vomitoxin and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Isolation and identification of Bacillus subtilis GXKCU1

[0037] (1) Aseptic operation Take 10 g of fermented mattress material from a dairy farm in Fujian and put it into a 250 mL Erlenmeyer flask, add 90 mL of sterile water, shake for 2 min, and make an initial suspension of 1:10.

[0038] (2) Put the initial suspension in a water bath at 80°C under normal pressure for 30 minutes, pipette 1mL of the initial suspension into a sterile test tube with a sterile pipette, add 9mL of sterile water, mix thoroughly to make a 1:10 dilution, and repeat the steps Made 10 -5 、10 -6 Diluent.

[0039] (3) Use a sterile pipette to draw 10 -5 、10 -6 0.1 mL of each dilution solution was inoculated on NA medium, spread with a sterile spreading rod, and stood at room temperature for 10 minutes to allow the inoculum to be absorbed by the agar. Invert the above plate and place it in an incubator at 35°C±1°C for 48h±2h.

[0040] according to Figure 1-3 It can be seen that the morph...

Embodiment 2

[0052] Application of Bacillus subtilis GXKCU1 in degrading vomitoxin

[0053] 1. Application of Bacillus subtilis GXKCU1 in degrading vomitoxin in different raw materials

[0054] After 40mL of soybean molasses dilution (soybean molasses: water = 1:1) and distiller's residue dilution (whole grains: water = 1:1) were autoclaved at 121°C for 30 minutes, 5% of Bacillus subtilis GXKCU1 was added. On day 0, day 5, day 10, and day 15, enzyme-linked immunosorbent assay (ELISA) was used to detect the corresponding vomitoxin content. The specific results are shown in Table 3, where Experiment 1 and Experiment 2 are parallel.

[0055] Table 3 Degradation rate of DON in soybean molasses by Bacillus subtilis GXKCU1

[0056]

[0057] From Table 3 and Figure 5 It can be seen that the Bacillus subtilis GXKCU1 of the present invention has a degradative effect on vomitoxin in soybean molasses, and the degradation rate can reach 26.82%.

[0058] 2. Application of Bacillus subtilis GXKCU...

Embodiment 3

[0078] Antagonism of Bacillus subtilis GXKCU1 against Fusarium graminearum

[0079] Test strain: Fusarium graminearum, from Guangdong Microbial Culture Collection Center.

[0080] Medium: PDA medium and optimized medium. The composition of the optimized medium is: 2% glucose, 1% peptone, 0.5% yeast powder, 1% sodium chloride, 0.2% potassium dihydrogen phosphate, 0.1% dipotassium hydrogen phosphate, and the pH is natural.

[0081] Bacillus subtilis GXKCU1 was inoculated into the optimized medium, and cultured at 35°C for 24 hours for later use.

[0082] Inoculate Fusarium graminearum into the PDA medium, and at the same time put a sterile small piece of paper in each of the four sides, select the upper and lower two sterile small pieces of paper, add 10 μL of Bacillus subtilis GXKCU1 bacterial solution dropwise, and the remaining two sterile paper pieces. Add 10 μL of sterile water dropwise to a small piece of bacterial paper, as a control group. After 4 days of constant tem...

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Abstract

The invention provides bacillus subtilis for degrading vomitoxin and application of the bacillus subtilis, and belongs to the technical field of microorganisms. The bacillus subtilis disclosed by the invention is named as bacillus subtilis GXKCU1, is preserved in the Guangdong Microbial Culture Collection Center, and has the preservation number of GDMCC (China General Microbiological Culture Collection Center) No.62040. The bacillus subtilis GXKCU1 disclosed by the invention can be fermented by taking vomitoxin as a fermentation substrate, and the vomitoxin is metabolized to synthesize high-added-value products such as theobromine, lupinine, cinnamyl alcohol and geraniol. According to the bacillus subtilis GXKCU1 disclosed by the invention, the highest degradation rate of the bacillus subtilis GXKCU1 on vomitoxin is 56.4%, and the harm of the vomitoxin can be effectively reduced.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to a bacillus subtilis for degrading vomitoxin and its application. Background technique [0002] The main component of vomitoxin is DON (deoxynivalenol, deoxynivalenol), which belongs to the trichothecene compound, mainly composed of Fusarium graminearum, Fusarium oxysporum, Fusarium moniliforme, Fusarium pseudocladoides, pink Produced by Fusarium fungi such as Fusarium spp. Because it can cause vomiting in pigs, it is also called vomitoxin. Due to its high cytotoxicity and immunosuppressive properties, DON poses a threat to human and animal health, especially has obvious effects on immune function. Depending on the dose and duration of exposure, DON can cause immunosuppression or immunostimulation. When people ingest DON-contaminated food, it will cause acute poisoning symptoms such as anorexia, vomiting, diarrhea, fever, unsteady standing, and unresponsiveness. In severe case...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/04C12P7/22C12P17/18A23L5/20A01N63/22A01P3/00C12R1/125
CPCC12N1/20C12P7/04C12P7/22C12P17/182A23L5/28A01N63/22
Inventor 蔡杏华罗莎莎易萍陈学文黄凤蝶陆书发秦瑞祥张晓露
Owner 广西优比特生物科技有限公司
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