Method for enhancing denitrification performance and metabolic activity of aerobic denitrifying bacteria under low-temperature condition
A technology of aerobic denitrifying bacteria and metabolic activity, applied in chemical instruments and methods, aerobic and anaerobic process treatment, water/sludge/sewage treatment, etc., can solve aerobic denitrifying bacteria denitrification and metabolic activity Inhibition, aerobic denitrification bacteria are difficult to become the dominant bacterial group, denitrification effect is not ideal, etc., to achieve the effect of improving aerobic denitrification performance, high application value and potential, and strengthening bacterial quorum sensing ability
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Embodiment 1
[0040] Put 300mL of water and 0.02mM of different electron shuttles (respectively: 1,5-AQDS, FA, AQ, LAW) into a 500mL conical flask, set up a blank control group without adding mediator, and adjust the initial pH range of the reaction system 7.0-7.2. The 5 conical flasks were sealed with a breathable sealing film, put into a high-pressure steam sterilizer for sterilization (121° C., 15 min), and cooled for later use. Pick a single colony of JQ1004 with good growth to LB medium, put it into a shaking incubator (30°C, 140r / min) for 12h, and then collect the bacteria by centrifugation. The OD600 of the cells was fixed with 0.9% (W / W) physiological saline and used as the inoculum. Take 10% (V / V) inoculum and inoculate it in the above-mentioned 5 conical flasks respectively. Continuous culture for 96h and sampling at regular intervals to measure the OD600 value and total nitrogen concentration of the bacterial liquid, and calculate the total nitrogen removal rate. Detection of ...
Embodiment 2
[0043] Pick the strain PHB18 to LB medium for activation and enrichment culture, inoculate its inoculum in a conical flask containing 0.02mM electron shuttle (respectively: 1,5-AQDS, FA, AQ, LAW), and culture continuously. Samples were taken at regular intervals for 96 hours, the OD600 value and total nitrogen concentration of the bacterial solution were measured, and the total nitrogen removal rate was calculated. Detection of bacterial biofilm formation ability (OD) at the 84th hour 570 ). The rest of the operation steps are the same as in case 1.
[0044] The effect of each mediator on the denitrification process of strain PHB18 at 2-4°C is as follows: Figure 4 As shown, the denitrification efficiency of bacteria reached the best when FA was added, and the average denitrification rate and total nitrogen removal rate were 0.25 mg / (L h) and 43.81%, and the total nitrogen content of the effluent was 29.02 mg / L. Compared with the control group, the total nitrogen content of...
Embodiment 3
[0046] Put 300mL of water and different concentrations (respectively: 0.02, 0.05, 0.10, 0.15mM) of FA into a 500mL conical flask, set up a blank control group without mediator, and adjust the initial pH range of the reaction system to 7.0-7.2. The 5 conical flasks were sealed with a breathable sealing film, put into a high-pressure steam sterilizer for sterilization (121° C., 15 min), and cooled for later use. Pick a single colony of PHB18 with good growth to LB medium, put it into a shaking incubator (30°C, 140r / min) for 12h, and then collect the cells by centrifugation. The OD600 of the cells was fixed with 0.9% (W / W) physiological saline to about 0.450 as the inoculum. Take 5% (V / V) inoculum and inoculate it in the above-mentioned 5 conical flasks respectively. Continuous culture for 72h and sampling at regular intervals to measure the OD600 value and total nitrogen concentration of the bacterial liquid, and calculate the total nitrogen removal rate. Detection of bacteria...
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